Inducible expression of chimpanzee prion protein (PrP) in murine PrP knock-out cells

Heinig, Lars; Mueller, Daniel A.; Ramljak, Sanja; Holznagel, Edgar; Stuke, Andreas W.

doi:10.1016/j.pep.2009.09.015

Cited by 2 publications

(2 citation statements)

References 53 publications

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“…PrP C is known to occur as three glycosylated variants having a molecular mass range of 27–35 kDa (15). Using the PrP antibody, however, western blot analysis yielded a single molecular mass band of approximately 28 kDa.…”

Section: Resultsmentioning

confidence: 99%

Expression of prion gene and presence of prion protein during development of mouse molar tooth germ

Khan

Press

Sehic

et al. 2010

European J Oral Sciences

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In order to gain insight into possible cellular functions of the prion protein (PrP) during normal development, the expression of Prnp (encoding the PrP) and the distribution of the PrP were studied in murine tooth germs. Expression of Prnp in the mouse first molar tooth germ was highly dynamic, increasing several-fold during the secretory phase of odontogenesis, exhibiting a time-course of expression similar to that of genes coding for other extracellular proteins [e.g. enamel matrix proteins (Amelx, Ambn, Enam), Aplp1, Clstn1, and Clu]. Western blot analysis suggested that the amounts of PrP and amyloid beta (A4) precursor-like protein 1 (APLP1) in the tooth germ followed time-courses similar to those of the corresponding mRNAs. Immunohistochemical studies of the distribution of PrP in murine molar and incisor tooth germs at embryonic day (E)18.5 suggested that this protein was located in the cervical loop, outer enamel epithelium, pre-ameloblasts, and dental papilla. Different degrees of immunolabelling of pre-ameloblasts on the mesial and distal aspects of a lower molar cusp may be related to different enamel configurations on the two aspects. It is concluded that the dynamic patterns of expression of Prnp, and of distribution of PrP, suggest that PrP may have functions during secretory odontogenesis, perhaps in relation to amelogenesis.

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Section: Resultsmentioning

confidence: 99%

Expression of prion gene and presence of prion protein during development of mouse molar tooth germ

Khan

Press

Sehic

et al. 2010

European J Oral Sciences

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“…al . [ 17 ] recently have shown that PrP res could be detected in the PrP 0/0 Chimp1 cell clone after incubation with BSE infected cow brain homogenate, this cell line has not been established as a persistently infected cell line against natural BSE infected bovine materials. Various cell lines have been developed which are susceptible to infection mainly with murine-adapted or ovine scrapie-infected materials and also recently two cell lines have been produced allowing infection with chronic wasting disease (CWD)[ 10 , 18 – 21 ].…”

Section: Discussionmentioning

confidence: 99%

Generation of a Persistently Infected MDBK Cell Line with Natural Bovine Spongiform Encephalopathy (BSE)

et al. 2015

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Bovine spongiform encephalopathy (BSE) is a zoonotic transmissible spongiform encephalopathy (TSE) thought to be caused by the same prion strain as variant Creutzfeldt-Jakob disease (vCJD). Unlike scrapie and chronic wasting disease there is no cell culture model allowing the replication of proteinase K resistant BSE (PrPBSE) and the further in vitro study of this disease. We have generated a cell line based on the Madin-Darby Bovine Kidney (MDBK) cell line over-expressing the bovine prion protein. After exposure to naturally BSE-infected bovine brain homogenate this cell line has shown to replicate and accumulate PrPBSE and maintain infection up to passage 83 after initial challenge. Collectively, we demonstrate, for the first time, that the BSE agent can infect cell lines over-expressing the bovine prion protein similar to other prion diseases. These BSE infected cells will provide a useful tool to facilitate the study of potential therapeutic agents and the diagnosis of BSE.

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Inducible expression of chimpanzee prion protein (PrP) in murine PrP knock-out cells

Cited by 2 publications

References 53 publications

Expression of prion gene and presence of prion protein during development of mouse molar tooth germ

Expression of prion gene and presence of prion protein during development of mouse molar tooth germ

Generation of a Persistently Infected MDBK Cell Line with Natural Bovine Spongiform Encephalopathy (BSE)

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