Expression of prion gene and presence of prion protein during development of mouse molar tooth germ

Khan, Qalb-E-Saleem; Press, Charles McL.; Sehic, Amer; Landin, Maria A.; Risnes, Steinar; Osmundsen, Harald

doi:10.1111/j.1600-0722.2010.00783.x

Cited by 6 publications

(8 citation statements)

References 44 publications

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“…The in situ results for Amelx , Amb n, and Enam mRNA showed similarities with immunohistochemical localization of Serpinb5, Clusterin, and Prnp (French et al, 1993; Davaadorj et al, 2010; Khan et al, 2010). Expression of enamel proteins in bone cells has previously been reported (Spahr et al, 2006; Tamburstuen et al, 2010; Tamburstuen et al, 2011) and is supported by the presented in situ data.…”

Section: Discussionsupporting

confidence: 52%

Gene Expression Profiling during Murine Tooth Development

Landin¹,

Shabestari²,

Babaie³

et al. 2012

Front. Gene.

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The aim of this study was to describe the expression of genes, including ameloblastin (Ambn), amelogenin X chromosome (Amelx), and enamelin (Enam) during early (pre-secretory) tooth development. The expression of these genes has predominantly been studied at post-secretory stages. Deoxyoligonucleotide microarrays were used to study gene expression during development of the murine first molar tooth germ at 24 h intervals, starting at the 11th embryonic day (E11.5), and up to the 7th day after birth (P7). The profile search function of Spotfire software was used to select genes with similar expression profile as the enamel genes (Ambn, Amelx, and Enam). Microarray results where validated using real-time reverse transcription-polymerase chain reaction (real-time RT-PCR), and translated proteins identified by Western-blotting. In situ localization of the Ambn, Amelx, and Enam mRNAs were monitored from E12.5 to E17.5 using deoxyoligonucleotide probes. Bioinformatics analysis was used to associate biological functions with differentially expressed (DE; p ≤ 0.05) genes. Microarray results showed a total of 4362 genes including Ambn, Amelx, and Enam to be significant DE throughout the time-course. The expression of the three enamel genes was low at pre-natal stages (E11.5–P0) increasing after birth (P1–P7). Profile search lead to isolation of 87 genes with significantly similar expression to the three enamel proteins. These mRNAs were expressed in dental epithelium and epithelium derived cells. Although expression of Ambn, Amelx, and Enam were lower during early tooth development compared to secretory stages enamel proteins were detectable by Western-blotting. Bioinformatic analysis associated the 87 genes with multiple biological functions. Around 35 genes were associated with 15 transcription factors.

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Section: Discussionsupporting

confidence: 52%

Gene Expression Profiling during Murine Tooth Development

Landin¹,

Shabestari²,

Babaie³

et al. 2012

Front. Gene.

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“…Our observations reported in this study are consistent with the previous findings that PrP C is localized in human and mouse odontoblasts (Manson et al, 1992, Schneider et al, 2007, as well as secretory ameloblasts in the developing tooth organ (Khan et al, 2010). However, our immunolocalization analysis showed PrP C highly localized in the apical border as well as lateral cell membranes, particularly in secretory odontoblasts and ameloblasts.…”

Section: Discussionsupporting

confidence: 81%

Multiple effects of the cellular prion protein on tooth development

Zhang

Kim²,

Opsahl-Vital³

et al. 2011

Int. J. Dev. Biol.

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The role of the prion protein (PrP) in transmissible spongiform encephalopathies has been the focus of intense investigation. However, less is known about the physiological function of normal cellular PrP (PrP C ). In adult human teeth, PrP C has been identified in odontoblasts, cementoblasts and epithelial remnants of Malassez. In this study, we have localized PrP C in developing human and mouse teeth, and investigated the function of PrP using a PrP-knockout (Prnp 0/0 ) mouse model. PrP C was detected in developing human and mouse ameloblasts and odontoblasts. In vitro, undifferentiated dental mesenchymal cells from embryonic day 18 (E18) Prnp 0/0 mouse molars proliferated much more rapidly compared to age-matched, wild-type (wt) mouse molar dental mesenchymal cells. Histochemistry and immunohistochemical analyses showed a subtle but measurable phenotype, with the absence of PrP resulting in earlier initiation of both dentin and enamel formation. Consistent with this finding, laser microdissected odontoblasts from newborn Prnp 0/0 mouse incisors had a reduced proliferation rate, as measured by the expression of proliferating cell nuclear antigen (PCNA), and increased type 1 collagen mRNA expression. Dentin microhardness of the fully erupted molars was reduced and incisal enamel mineralization was delayed in Prnp 0/0 compared to age-matched wt mouse teeth. Taken together, these results suggest that PrP C affects multiple processes involved in tooth formation, through regulating the differentiation of ameloblasts and odontoblasts.

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“…Using microarray expression data from molar tooth germs from mice treated with anti‐miR‐214, a profile search using the results for Clu and Tgfb1 as search criteria led to the isolation of a cluster of 35 genes, which also included Ambn , Amelx , and Enam . Eight of the genes [ Ambn ; Amelx ; Clu ; Enam ; laminin, beta 3 ( Lamb3 ); papilin, proteoglycan‐like sulfated glycoprotein ( Papln ); Prnp ; and solute carrier family 25 (mitochondrial carrier, glutamate), member 22 ( Slc25a22 )] had also been observed in a previous study .…”

Section: Resultsmentioning

confidence: 81%

“…In a previous study, expression of the prion protein ( Prnp ) gene in murine molar tooth germs was shown to increase at postnatal developmental stages. The time‐course of expression of Prnp was similar to that of genes encoding enamel proteins [ameloblastin ( Ambn ), amelogenin X chromosome ( Amelx ), and enamelin ( Enam )] and also to that of clusterin ( Clu ) . In contrast, genes associated with regulation of organ growth [e.g.…”

mentioning

confidence: 92%

Expression of Clu and Tgfb1 during murine tooth development: effects of in‐vivo transfection with anti‐miR‐214

Khan

Sehic

Risnes

et al. 2013

European J Oral Sciences

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Expression of clusterin (Clu) in the murine first molar tooth germ was markedly increased at postnatal developmental stages. The time-course of expression of this gene paralleled those of other genes encoding proteins involved during the secretory phase of odontogenesis, as described previously. Immunohistochemical studies of clusterin in murine molar tooth germs suggested this protein to be located in outer enamel epithelium, regressing enamel organ, secretory ameloblasts, and the dental epithelium connecting the tooth to the oral epithelium at an early eruptive stage. Immunolabelling of transforming growth factor beta-1 (TGF-β1) revealed it to be located close to clusterin. The levels of expression of Clu and Tgfb1 were markedly decreased following in-vivo transfection with anti-miR-214. In contrast, the expression of several genes associated with regulation of growth and development were increased by this treatment. We suggest that clusterin has functions during secretory odontogenesis and the early eruptive phase. Bioinformatic analysis after treatment with anti-miR-214 suggested that, whilst cellular activities associated with tooth mineralization and eruption were inhibited, activities associated with an alternative developmental activity (i.e. biosynthesis of contractile proteins) appeared to be stimulated. These changes probably occur through regulation mediated by a common cluster of transcription factors and support suggestions that microRNAs (miRNAs) are highly significant as regulators of differentiation during odontogenesis.

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Expression of prion gene and presence of prion protein during development of mouse molar tooth germ

Cited by 6 publications

References 44 publications

Gene Expression Profiling during Murine Tooth Development

Gene Expression Profiling during Murine Tooth Development

Multiple effects of the cellular prion protein on tooth development

Expression of Clu and Tgfb1 during murine tooth development: effects of in‐vivo transfection with anti‐miR‐214

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