1988
DOI: 10.1093/nar/16.3.953
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Inducible DNA-protein interactions of the murine kappa immunoglobulin enhancer in intact cells: comparison within vitrointeractions

Abstract: The large intron of the kappa immunoglobulin gene contains a cis-acting enhancer element, which is important in the tissue-specific expression of the gene. We have confirmed the binding activity of a sequence-specific factor present in lymphoid extracts derived from cell lines expressing, or induced to express, the kappa gene. We have extended these studies to show the binding activity is present in normal activated splenic B cells as well as lambda producing cells, and have demonstrated by DNAse footprint ana… Show more

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Cited by 17 publications
(14 citation statements)
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“…A footprint was also observed over an A/T-rich sequence situated between the E* and EO sites which does not contain defined factor binding sites. As previously reported, we could not detect protein interaction with the E1 and E2 boxes using this technique (32).…”
Section: The Mouse and Rabbit Enhancers Do Not Interact With The Samesupporting
confidence: 48%
“…A footprint was also observed over an A/T-rich sequence situated between the E* and EO sites which does not contain defined factor binding sites. As previously reported, we could not detect protein interaction with the E1 and E2 boxes using this technique (32).…”
Section: The Mouse and Rabbit Enhancers Do Not Interact With The Samesupporting
confidence: 48%
“…Additional proteins which bind within this region of the kappa enhancer have been observed (12,23,24). Currie and Roeder have identified a nuclear factor, NF-KE*, which was found to be more abundant in HeLa cells than in B cells (12).…”
Section: Discussionmentioning
confidence: 99%
“…Protein concentrations were determined as described by Lowry (44). Nuclear binding factors were analyzed by their altered mobility on acrylamide gels as previously described (34). Double stranded oligonucleotides used included a 34 bp sequence containing the NF-xB binding site (34, sense strand: 5'-TCTCAACAGAGGGGACTTTCCGAGAGCCATCTGG-3') and a 27 bp duplex containing the octamer sequence motif in the context of the 70Z/3 kappa promoter sequence (25, sense strand: 5'-GTATCTTGC-GATTTGCATATTACATTT-3').…”
Section: Northern Blot Analysismentioning
confidence: 99%
“…Within the n enhancer, one sequence motif which appears essential for enhancer function is identical to the 11 bp SV-40 core enhancer sequence, and a specific factor whose binding correlates with K expression has been identified and designated NF-nB (31)(32)(33). This factor has been demonstrated in mature B cell extracts and shown to be inducible by LPS in the pre-B cell line, 70Z/3 as well as normal B lymphocytes (34).…”
mentioning
confidence: 99%