Induced polyploidy and broad variation in phytochemical traits and altered gene expression in Salvia multicaulis

Tavan, Mansoureh; Azizi, Ali; Sarikhani, Hassan; Mirjalili, Mohammad Hossein; Rigano, Maria Manuela

doi:10.1016/j.scienta.2021.110592

Cited by 14 publications

(8 citation statements)

References 83 publications

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“…It was estimated that about 95% of ferns, 15% of gymnosperms, and 70% of angiosperms have experienced chromosomes doubling in their evolutionary history ( Masterson, 1994 ; Wendel, 2000 ). Polyploidization has been demonstrated to introduce profound phenotypic alterations including morphological ( Ramsey and Schemske, 2002 ; Ye et al, 2010 ; Catalano et al, 2021 ), physiological ( Balal et al, 2017 ; Mattingly and Hovick, 2021 ), phytochemical ( Pradhan et al, 2018 ; Zheng et al, 2021 ; Tavan et al, 2022 ), and molecular ( Adams and Wendel, 2005 ; Chen and Ni, 2006 ; Yan et al, 2019 ; Zhang et al, 2022 ) characteristics. In recent years, polyploidization has become a powerful breeding strategy to enable the development of new and improved germplasm, even cultivars.…”

Section: Introductionmentioning

confidence: 99%

“…Due to the absence of adequate breeding method to introduce desirable variations, it has been a long time that new garlic varieties are selected only from existing living collections through natural or induced mutations ( Shemesh-Mayer and Kamenetsky-Goldstein, 2021 ). Increasing ploidy by artificial polyploidy induction (APPI) is an efficient way to create superior plants to sterile plants such as garlic by improving the morphology, disease resistance, adaptability to environmental stress, and yield or quality ( Balal et al, 2017 ; Zhou et al, 2020 ; Kim et al, 2021 ; Tavan et al, 2022 ). In spite of few reports concerning APPI for garlic germplasm innovation ( Novák, 1983 ; Cheng et al, 2012 ), they mainly focused on induction protocols with single antimitotic chemical and basic ploidy determination instead of performance evaluation, especially physiological and phytochemical characteristics.…”

Section: Introductionmentioning

confidence: 99%

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In vitro Induction and Phenotypic Variations of Autotetraploid Garlic (Allium sativum L.) With Dwarfism

et al. 2022

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Garlic (Allium sativum L.) is a compelling horticultural crop with high culinary and therapeutic values. Commercial garlic varieties are male-sterile and propagated asexually from individual cloves or bulbils. Consequently, its main breeding strategy has been confined to the time-consuming and inefficient selection approach from the existing germplasm. Polyploidy, meanwhile, plays a prominent role in conferring plants various changes in morphological, physiological, and ecological properties. Artificial polyploidy induction has gained pivotal attention to generate new genotype for further crop improvement as a mutational breeding method. In our study, efficient and reliable in vitro induction protocols of autotetraploid garlic were established by applying different antimitotic agents based on high-frequency direct shoot organogenesis initiated from inflorescence explant. The explants were cultured on solid medium containing various concentrations of colchicine or oryzalin for different duration days. Afterward, the ploidy levels of regenerated plantlets with stable and distinguished characters were confirmed by flow cytometry and chromosome counting. The colchicine concentration at 0.2% (w/v) combined with culture duration for 20 days was most efficient (the autotetraploid induction rate was 21.8%) compared to the induction rate of 4.3% using oryzalin at 60 μmol L–1 for 20 days. No polymorphic bands were detected by simple sequence repeat analysis between tetraploid and diploid plantlets. The tetraploids exhibited a stable and remarkable dwarfness effect rarely reported in artificial polyploidization among wide range of phenotypic variations. There are both morphological and cytological changes including extremely reduced plant height, thickening and broadening of leaves, disappearance of pseudostem, density reduction, and augmented width of stomatal. Furthermore, the level of phytohormones, including, indole propionic acid, gibberellin, brassinolide, zeatin, dihydrozeatin, and methyl jasmonate, was significantly lower in tetraploids than those in diploid controls, except indole acetic acid and abscisic acid, which could partly explain the dwarfness in hormonal regulation aspect. Moreover, as the typical secondary metabolites of garlic, organosulfur compounds including allicin, diallyl disulfide, and diallyl trisulfide accumulated a higher content significantly in tetraploids. The obtained dwarf genotype of autotetraploid garlic could bring new perspectives for the artificial polyploids breeding and be implemented as a new germplasm to facilitate investigation into whole-genome doubling consequences.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In vitro Induction and Phenotypic Variations of Autotetraploid Garlic (Allium sativum L.) With Dwarfism

et al. 2022

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“…As a result, while the diploid species contained 14 distinct essential oils, the tetraploid developed with eight additional compounds (Estaji et al, 2017). An increase in aromatic compounds was also reported in S. muticaulis (Tavan et al, 2022) and other polyploid-induced plants (Dhawan and Lavania, 1996). Therefore, the quantity and diversity of essential oils may be increased by inducing polyploidy in S. coccinea.…”

Section: Resultsmentioning

confidence: 75%

Improved Floral Characteristics of Salvia coccinea through Intraspecific Hybridization

Maynard

Ruter

2022

horts

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Salvia is the largest genus in the Lamiaceae with more than 1000 species. The species S. coccinea used in this study has naturalized in the southeastern United States and is an important plant for pollinators. This project aimed to improve phenotypic characteristics of S. coccinea for use in the landscape by selecting for increased petal size and unique petal color. Two elite accessions were selected for hybridization using the pedigree method. One selection displayed compact habit with bicolored coral and white flowers, while the other was slightly larger with solid red flowers. Selections were made based on improved flower color and larger petal size. The breeding program achieved a 25% increase in petal width and a more vivid petal color for the coral bicolored selections. Additionally, a 60% increase in petal width was achieved for red flowers. These novel selections are attractive plants for the landscape, displaying improved ornamental value and supporting local pollinator populations.

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“…This represents the consistency of the chemical composition of the same species [ 32 ]. This is because the chemical composition of different species is regulated by genes [ 33 ]. However, the levels of these chemicals can vary depending on factors such as soil, sunlight, and moisture.…”

Section: Resultsmentioning

confidence: 99%

Analysis of Electrochemically Active Substances in Malvaceae Leaves via Electroanalytical Sensing Technology for Species Identification

Wang

et al. 2023

Micromachines

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Electrochemical analysis has become a new method for plant analysis in recent years. It can not only collect signals of electrochemically active substances in plant tissues, but can also be used to identify plant species. At the same time, the signals of electrochemically active substances in plant tissues can also be used to investigate plant phylogeny. In this work, we collected electrochemical finger patterns in Malvaceae leaves based on the established methodological strategy. After the second derivative treatment, the collected electrochemical fingerprints can show more obvious differences. Three different recognition models were used to attempt electrochemical fingerprinting. The results show that linear support vector classification can be used to identify species with high accuracy by combining the electrochemical fingerprint signals collected in the phosphoric acid buffer solution and acetic acid buffer solution. In addition, the fingerprint information collected by the electrochemical sensor is further used for phylogenetic investigation. The 18 species were divided into three clusters. Species of the same genus have been clustered together. Dendrogram obtained by electrochemical fingerprinting was used to compare previously reported results deduced from morphological and complete chloroplast genomes.

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Induced polyploidy and broad variation in phytochemical traits and altered gene expression in Salvia multicaulis

Cited by 14 publications

References 83 publications

In vitro Induction and Phenotypic Variations of Autotetraploid Garlic (Allium sativum L.) With Dwarfism

In vitro Induction and Phenotypic Variations of Autotetraploid Garlic (Allium sativum L.) With Dwarfism

Improved Floral Characteristics of Salvia coccinea through Intraspecific Hybridization

Analysis of Electrochemically Active Substances in Malvaceae Leaves via Electroanalytical Sensing Technology for Species Identification

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