Induced Pluripotent Stem Cell-Derived Corneal Cells: Current Status and Application

Mahmood, Nasif; Suh, Taylor C.; Ali, Kiran M.; Sefat, Eelya; Jahan, Ummay Mowshome; Huang, Yihan; Gilger, Brian C.; Gluck, Jessica M.

doi:10.1007/s12015-022-10435-8

Cited by 11 publications

(5 citation statements)

References 121 publications

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“…Perinatal stem cells, including amniotic fluid stem cells, exhibit multipotent differentiation capabilities. Additionally, umbilical cord blood stem cells are employed in hematopoietic stem cell transplantation, while cord tissue stem cells (mesenchymal stem cells), dental pulp stem cells (multipotent stem cells), and ADSCs are also notable sources [11][12][13][14][15][16][17][18][19]. Several concerns have been raised regarding the procurement of human embryonic stem cells.…”

Section: Introductionmentioning

confidence: 99%

Adipose-Derived Stem Cells: Angiogenetic Potential and Utility in Tissue Engineering

Biniazan,

Stoian,

Haykal

2024

IJMS

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Adipose tissue (AT) is a large and important energy storage organ as well as an endocrine organ with a critical role in many processes. Additionally, AT is an enormous and easily accessible source of multipotent cell types used in our day for all types of tissue regeneration. The ability of adipose-derived stem cells (ADSCs) to differentiate into other types of cells, such as endothelial cells (ECs), vascular smooth muscle cells, or cardiomyocytes, is used in tissue engineering in order to promote/stimulate the process of angiogenesis. Being a key for future successful clinical applications, functional vascular networks in engineered tissue are targeted by numerous in vivo and ex vivo studies. The article reviews the angiogenic potential of ADSCs and explores their capacity in the field of tissue engineering (TE).

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Section: Introductionmentioning

confidence: 99%

Adipose-Derived Stem Cells: Angiogenetic Potential and Utility in Tissue Engineering

Biniazan,

Stoian,

Haykal

2024

IJMS

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“…Guiding human pluripotent stem cells (hPSCs) toward specific cell types such as LSCs possesses significant promise for treating patients needing LSC transplants. Major advantages of hPSCs as the LSC source include abundance and donor-independent “off-the-shelf” nature, and, to this day, multiple CE/LSC differentiation methods for hPSCs have been introduced ( Mahmood et al., 2022 ). Aiming to facilitate clinical translation, protocols have shifted toward simplified feeder- and serum-free versions such as our own ( Hongisto et al., 2017 ; Mikhailova et al., 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Deciphering the heterogeneity of differentiating hPSC-derived corneal limbal stem cells through single-cell RNA sequencing

Vattulainen,

Smits,

Arts

et al. 2024

Stem Cell Reports

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“…Corneal limbal stem cell (LSC)-driven repair mechanisms and homeostatic renewal of corneal epithelium (CE) are essential processes in the maintenance of human ocular surface health and clarity (Deng et al, 2019). Guiding human pluripotent stem cells (hPSCs) toward specific cell types such as LSCs possesses significant promise in translational applications and could be used for ocular surface regeneration for patients suffering from bilateral limbal stem cell deficiency (LSCD) (Ghareeb et al, 2020; Mahmood et al, 2022). Major advantages of hPSCs as the LSC source include abundance and donor-independent “off-the-shelf” nature, and to this day, multiple CE/LSC differentiation methods for hPSCs have been introduced (Mahmood et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

“…Guiding human pluripotent stem cells (hPSCs) toward specific cell types such as LSCs possesses significant promise in translational applications and could be used for ocular surface regeneration for patients suffering from bilateral limbal stem cell deficiency (LSCD) (Ghareeb et al, 2020; Mahmood et al, 2022). Major advantages of hPSCs as the LSC source include abundance and donor-independent “off-the-shelf” nature, and to this day, multiple CE/LSC differentiation methods for hPSCs have been introduced (Mahmood et al, 2022). In an attempt to facilitate their clinical translation, some protocols have progressed from traditional reliance on conditioned media, feeder cells and/or serum (e.g.…”

Section: Introductionmentioning

confidence: 99%

Deciphering the heterogeneity of differentiating hPSC-derived corneal limbal stem cells through single-cell RNA-sequencing

Vattulainen,

Smits,

Lima Cunha

et al. 2023

Preprint

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SummaryA comprehensive understanding of the human pluripotent stem cell (hPSC) differentiation process stands as a prerequisite for the development hPSC-based therapeutics. In this study, single-cell RNA-sequencing (scRNA-seq) was performed to decipher the heterogeneity during differentiation of three hPSC lines towards corneal limbal stem cells (LSCs). The scRNA-seq data revealed nine clusters encompassing the entire differentiation process, among which five followed the anticipated differentiation path of LSCs. The remaining four clusters were previously undescribed cell states that were annotated as either mesodermal-like or undifferentiated subpopulations, and their prevalence was hPSC line-dependent. Distinct cluster-specific marker genes identified in this study were confirmed by immunofluorescence analysis and employed to purify hPSC-derived LSCs, which effectively minimized the variation in the line-dependent differentiation efficiency. In summary, scRNA-seq offered molecular insights into the heterogeneity of hPSC-LSC differentiation, allowing a data-driven strategy for consistent and robust generation of LSCs, essential for future advancement toward clinical translation.HighlightshPSCs to LSCs spans epithelial, mesodermal, and undifferentiated cell states.scRNA-seq reveals the cell line-dependent differentiation heterogeneity.ITGA6 and AREG can be used to select pure LSC-like subpopulation.

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Induced Pluripotent Stem Cell-Derived Corneal Cells: Current Status and Application

Cited by 11 publications

References 121 publications

Adipose-Derived Stem Cells: Angiogenetic Potential and Utility in Tissue Engineering

Adipose-Derived Stem Cells: Angiogenetic Potential and Utility in Tissue Engineering

Deciphering the heterogeneity of differentiating hPSC-derived corneal limbal stem cells through single-cell RNA sequencing

Deciphering the heterogeneity of differentiating hPSC-derived corneal limbal stem cells through single-cell RNA-sequencing

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