Induced Biosynthesis of Uricase in Yeast

Roush, Allan H.; Domnas, A.

doi:10.1126/science.124.3212.125

Cited by 30 publications

(7 citation statements)

References 7 publications

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“…The normal intestinal flora include A. aerogenes and Bacillus species. Hence, these results, along with those of other studies with Clostridium (2, 8) and yeast (9,10), support the hypothesis that uric acid, if administered orally to humans, is decomposed by intestinal bacteria (6,11). However, the importance of aerobic decomposition of uric acid in the intestinal tract by these organisms has not yet been ascertained.…”

Section: Discussionsupporting

confidence: 86%

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Degradation of Uric Acid by Certain Aerobic Bacteria

Rouf

Lomprey

1968

J Bacteriol

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We have isolated and identified nine cultures of aerobic bacteria capable of growing on an elective medium containing uric acid as the only source of carbon, nitrogen, and energy. Four of these cultures were identified as Aerobacter aerogenes, two as Klebsiella pneumoniae, and the remainder as Serratia kiliensis, Pseudomonas aeruginosa, and Bacillus species. Another culture identified as P. fluorescens required both glucose and uric acid for growth. When 23 laboratory stock cultures were inoculated into the uric acid medium, A. aerogenes, B. subtilis, Mycobacterium phlei, P. aeruginosa, and S. marcescens were able to grow. These five cultures also grew when the uric acid was replaced with adenine, guanine, hypoxanthine, xanthine, or allantoin, but growth was poor. In all of these media, including the uric acid medium, addition of glucose along with the nitrogenous compounds yielded good growth. Induction experiments demonstrated that the ability of A. aerogenes, K. pneumoniae, P. aeruginosa, P. fluorescens, S. kiliensis, S. marcescens, B. subtilis, and Bacillus sp. to degrade uric acid is an induced property. Of these organisms, only Bacillus sp. accumulated a small amount of intracellular uric acid.

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Section: Discussionsupporting

confidence: 86%

“…In yeast, uptake of uric acid from the surrounding medium, and subsequent increase in uricase activity in cellular extracts and breakdown of uric acid have been noted (9,10).…”

mentioning

confidence: 99%

Degradation of Uric Acid by Certain Aerobic Bacteria

Rouf

Lomprey

1968

J Bacteriol

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“…Addition of uric acid to the growth medium enhanced enzyme formation fourto fivefold. A similar effect of uric acid was reported in bacteria (4, 9), molds (5,16), and a yeast (15). On the other hand, Itaya et al (6) demonstrated that uricase production increased when resting cells of C. utilis were incubated in an induction medium consisting of glucose, Na2HPO4, KCl, MgSO4, and uric acid.…”

Section: Resultssupporting

confidence: 66%

Production of uricase by Candida tropicalis using n-alkane as a substrate

Tanaka¹,

Yamamura²,

Kawamoto³

et al. 1977

Appl Environ Microbiol

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Production of uricase (urate oxidase, EC 1.7.3.3) by n-alkane-utilizing Candida tropicalis pK233 was studied. Although the yeast showed very low enzyme productivity under growing conditions on glucose or an n-alkane mixture (C,O to C13) (<2 U/g of dry cells), enzyme formation was enhanced markedly in an induction medium consisting of potassium phosphate buffer, MgSO4, uric acid, and an n-alkane mixture (47 U/g of dry cells) or glucose (21 U/g of dry cells). Of the carbon sources tested, the n-alkane mixture was the most suitable for enzyme production. Appropriate aeration also stimulated uricase formation. In addition to uric acid, xanthine, guanine, adenine, and hypoxanthine were also effective for inducing uricase. Under optimum conditions, the maximum yield of the enzyme was 91 U/g of dry cells. Uricase thus induced was localized in the microbodies of the yeast.

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“…Like S. cerevisiae , transport of glucose into K. lactis has been shown to be by facilitated diffusion 319, 320: the gene HGT1 19 encodes a constitutive high‐affinity carrier41 with K m = 1 m M 396 and RAG1 20 encodes a low‐affinity glucose carrier which is induced by glucose127, K m = 20–50 m M 396. Anja Diezemann and Eckhard Boles speculate that Hgt1p may be proton‐coupled, as is the fructose carrier Frt1p, which they described [95, p. 287].…”

Section: Transport Into Various Yeast Speciesmentioning

confidence: 99%

A history of research on yeasts 13. Active transport and the uptake of various metabolites¹

Barnett

2008

Yeast

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Induced Biosynthesis of Uricase in Yeast

Cited by 30 publications

References 7 publications

Degradation of Uric Acid by Certain Aerobic Bacteria

Degradation of Uric Acid by Certain Aerobic Bacteria

Production of uricase by Candida tropicalis using n-alkane as a substrate

A history of research on yeasts 13. Active transport and the uptake of various metabolites¹

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Induced Biosynthesis of Uricase in Yeast

Cited by 30 publications

References 7 publications

Degradation of Uric Acid by Certain Aerobic Bacteria

Degradation of Uric Acid by Certain Aerobic Bacteria

Production of uricase by Candida tropicalis using n-alkane as a substrate

A history of research on yeasts 13. Active transport and the uptake of various metabolites1

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A history of research on yeasts 13. Active transport and the uptake of various metabolites¹