Production of uricase by Candida tropicalis using n-alkane as a substrate

Abstract

Production of uricase (urate oxidase, EC 1.7.3.3) by n-alkane-utilizing Candida tropicalis pK233 was studied. Although the yeast showed very low enzyme productivity under growing conditions on glucose or an n-alkane mixture (C,O to C13) (<2 U/g of dry cells), enzyme formation was enhanced markedly in an induction medium consisting of potassium phosphate buffer, MgSO4, uric acid, and an n-alkane mixture (47 U/g of dry cells) or glucose (21 U/g of dry cells). Of the carbon sources tested, the n-alkane mixture was the most suitable for enzyme production. Appropriate aeration also stimulated uricase formation. In addition to uric acid, xanthine, guanine, adenine, and hypoxanthine were also effective for inducing uricase. Under optimum conditions, the maximum yield of the enzyme was 91 U/g of dry cells. Uricase thus induced was localized in the microbodies of the yeast.

Uric acid in plants and microorganisms: Biological applications and genetics - A review

“…Most vertebrates possess uricase, except humans and higher apes, which became not functional by point mutation during evolution resulting in the formation of a redundant protein [25] . Uricase is localized inside microorganisms, especially Bacillus pasteurii [26] , Proteus mirabilis [27] , and Escherichia coli [28] , while other microorganisms could produce them extracellularly by changing certain components of the culture media as in Streptomyces albosriseolus [29] , Microbacterium [30] , Bacillus thermocatenulatus [31] , Candida tropicalis [32] , and Pseudomonas aeruginosa [33] .…”

Medium optimization for extracellular urate oxidase production by a newly isolated Aspergillus Niger

“…These days many fungal sources have been used for uricase production such as Candida tropicalis [11], Aspergillus flavus [36], and Candida utilis [37]. In this study, 40 soil samples were collected and microorganisms with uricase production ability were isolated for studying optimized conditions for increasing uricase production.…”

“…Microorganisms such as bacteria and fungi normally have uricase enzymes [7], so they are used for uricase production. Some of the microorganisms used to produce uricase are Streptomyces exfoliatus [9], Pseudomonas aeruginosa [7], Bacillus subtilis [10], Candida tropicalis [11], and Aspergillus flavus [8]. Wheat, chickpea, and broad bean are plant sources, which were studied for uricase production and isolation [5].…”

Production and characterization of uricase from Streptomyces exfoliatus UR10 isolated from farm wastes

“…A thermostable uricase was obtained from Microbacterium (7) and Bacillus thermocatenulatus (8). Tanaka et al (9) demonstrated that in addition to uric acid, xanthine, guanine, adenine, and hypoxanthine were also effective for inducing uricase in Candida tropicalis. Abdel-Fattah et al (10) found that glucose, the medium used, pH, CuSO 4 , and FeSO 4 all had a highly significant effect on the uricase activity produced by Pseudomonas aeruginosa, based on statistical experimental designs.…”