Induced alteration of rat erythrocyte membrane with effect of pyrethroid based compounds

Liu, Hui; Hussain, Shaik Althaf; Ali, Daoud; Alomar, Suliman Yousef; Shaik, Umeshareen; Alghamdi, Hamzah A.; Maddu, Narendra

doi:10.1016/j.sjbs.2020.08.011

Cited by 8 publications

(9 citation statements)

References 28 publications

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“…A significant increase (about 3.5 folds) in the content of GSH in rats erythrocytes after CYP treatment showed pro-oxidant conditions in the RBCs, which may be an initial adaptive response to increased oxidative stress in CYP-treated erythrocytes (Kale et al, 1999). Our results in the same way with Liu et al (2020) who found that treating rats with pyrethroid insecticides tetramethrin and prallethrin induced elevation of GSH in erythrocytes.…”

Section: Discussionsupporting

confidence: 88%

In Vitro Effect of Cypermethrin on the Perturbations of Rat Erythrocytes: The Ameliorative Role of Carvone

Hamed

Mosallam

Abdel-Razik

2022

Alexandria Science Exchange Journal

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Erythrocytes are a suitable model to study the membrane oxidative injury resulted by different xenobiotic. An attempt was made to study the antioxidant property of carvone (CAR), a monoterpene presents in herbs to mitigate toxicity of cypermethrin (CYP), a type II pyrethroid, in male rat erythrocytes in vitro. Erythrocytes were prepared and exposed to CYP (25-800 μM) alone and together with CAR (40 and 320 μM). Results showed superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities were significantly decreaseed. Lipid peroxidation (LPO) level, reduced glutathione (GSH) content, both of glutathione stransfarase (GST), and glutathione reductase (GR) activities were significantly increased in comparison of control value. Scanning electron microscope (SEM) supported the toxic effect of CYP which presented various degrees of alteration in shape and ruptured membranes. Pre-treatment of erythrocytes with CAR induced alleviation in all the tested parameters. It can be recommended that CYP induced oxidative stress in vitro in rat erythrocytes and supplementation with CAR ameliorated these effects.

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Section: Discussionsupporting

confidence: 88%

In Vitro Effect of Cypermethrin on the Perturbations of Rat Erythrocytes: The Ameliorative Role of Carvone

Hamed

Mosallam

Abdel-Razik

2022

Alexandria Science Exchange Journal

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“…[36] Alterations in plasma profile and lipid homeostasis were evident in rats exposed to tetramethrin and prallethrin via the nasal route. [37] Retention of native and oxidized LDL in the aorta, altered levels of cholesterol and HDL due to permethrin or bifenthrin induced toxicity in rats, [38,39] disturbances in the plasma biomarkers (inflammatory cytokines and that of liver function) by deltamethrin, [40] increased levels of plasma cholesterol and triglycerides in rats exposed to cypermethrin [41] and DNA damage in the peripheral lymphocytes of farmers who sprayed pesticides (deltamethrin) [42] are some recent reports related to the effects of pyrethroids on the hematological parameters. In this study, we observed disturbances in the levels of serum biomarkers that define liver function and lipid reported in apparently healthy subjects in Japan.…”

Section: Discussionmentioning

confidence: 99%

Effect of a mixture of pyrethroids at doses similar to human exposure through food in the Indian context

Ravula

Yenugu

2022

J Biochem & Molecular Tox

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Residual amounts of pyrethroids were detected in rice and vegetables of the Indian market. Thus, consumers are exposed to a mixture of pyrethroids on a daily basis through food. Though a large number of studies reported the toxic effects of pyrethroids, there are no reports that used doses equivalent to human consumption. In this study, male Wistar rats were exposed daily to a mixture of pyrethroids for 1-15 months which is equivalent to the amount present in rice and vegetables consumed by an average Indian each day. The oxidant-antioxidant status (lipid peroxidation, nitric oxide; activities of catalase, glutathione peroxidase, glutathione S transferase, and superoxide dismutase) and anatomical changes in the general organs (liver, lung, and kidney) and male reproductive tract tissues (caput, cauda, testis, and prostate) were evaluated. Further, liver and kidney function tests, lipid profile, and complete blood picture were analyzed.Increased oxidative stress, perturbations in the antioxidant enzyme activities, and damage to the anatomical architecture were observed. Disturbances in the liver function and lipid profile were significant. Results of our study demonstrate that exposure to a mixture of pyrethroids at a dose that is equivalent to human consumption can cause systemic and reproductive toxicity, which may ultimately result in the development of lifestyle diseases. This first line of evidence will fuel further studies to determine the impact of food-based pyrethroid exposure on the long-term health of humans and to envisage policies to reduce pesticide content in food products.

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“…The present study identified a novel bacterial isolate G. cholesterolivorans A16 with strong tetramethrin degradation activity. Strain A16 utilized tetramethrin as the sole car-bon source for its growth over a wide range of temperatures (20-40 • C) and pH values (5)(6)(7)(8)(9), indicating that the strain has excellent applicability in different environments. It is noteworthy that strain A16 tolerated and degraded tetramethrin up to a concentration of 800 mg•L −1 , thus giving it an exceptional ability to colonize ecological niches where pesticide concentrations are high.…”

Section: Discussionmentioning

confidence: 99%

“…Strain A16 was capable of rapidly degrading tetramethrin without a lag phase over a wide range of temperatures (20-40 • C) and pH values (5)(6)(7)(8)(9). This is an important feature of bioremediation by organisms in a variable environment [41].…”

Section: Optimization Of Tetramethrin Degradation Conditionsmentioning

confidence: 99%

“…Pyrethroids are highly hydrophobic and are quickly adsorbed into sediments when they enter water [8]. Studies have shown that pesticides of pyrethroids can induce changes in the erythrocyte membrane in rats, resulting in a significant increase in erythrocyte membrane phospholipids and a decrease in cholesterol levels [9]. At the same time, deltamethrin significantly inhibited the hatching rate of carp at a concentration of 50 µg•L −1 , with a hatching rate of only 9% [10].…”

Section: Introductionmentioning

confidence: 99%

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Novel Mechanism and Kinetics of Tetramethrin Degradation Using an Indigenous Gordonia cholesterolivorans A16

Guo

Huang

Pang

et al. 2021

IJMS

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Tetramethrin is a pyrethroid insecticide that is commonly used worldwide. The toxicity of this insecticide into the living system is an important concern. In this study, a novel tetramethrin-degrading bacterial strain named A16 was isolated from the activated sludge and identified as Gordonia cholesterolivorans. Strain A16 exhibited superior tetramethrin degradation activity, and utilized tetramethrin as the sole carbon source for growth in a mineral salt medium (MSM). High-performance liquid chromatography (HPLC) analysis revealed that the A16 strain was able to completely degrade 25 mg·L−1 of tetramethrin after 9 days of incubation. Strain A16 effectively degraded tetramethrin at temperature 20–40 °C, pH 5–9, and initial tetramethrin 25–800 mg·L−1. The maximum specific degradation rate (qmax), half-saturation constant (Ks), and inhibition constant (Ki) were determined to be 0.4561 day−1, 7.3 mg·L−1, and 75.2 mg·L−1, respectively. The Box–Behnken design was used to optimize degradation conditions, and maximum degradation was observed at pH 8.5 and a temperature of 38 °C. Five intermediate metabolites were identified after analyzing the degradation products through gas chromatography–mass spectrometry (GC-MS), which suggested that tetramethrin could be degraded first by cleavage of its carboxylester bond, followed by degradation of the five-carbon ring and its subsequent metabolism. This is the first report of a metabolic pathway of tetramethrin in a microorganism. Furthermore, bioaugmentation of tetramethrin-contaminated soils (50 mg·kg−1) with strain A16 (1.0 × 107 cells g−1 of soil) significantly accelerated the degradation rate of tetramethrin, and 74.1% and 82.9% of tetramethrin was removed from sterile and non-sterile soils within 11 days, respectively. The strain A16 was also capable of efficiently degrading a broad spectrum of synthetic pyrethroids including D-cyphenothrin, chlorempenthrin, prallethrin, and allethrin, with a degradation efficiency of 68.3%, 60.7%, 91.6%, and 94.7%, respectively, after being cultured under the same conditions for 11 days. The results of the present study confirmed the bioremediation potential of strain A16 from a contaminated environment.

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Induced alteration of rat erythrocyte membrane with effect of pyrethroid based compounds

Cited by 8 publications

References 28 publications

In Vitro Effect of Cypermethrin on the Perturbations of Rat Erythrocytes: The Ameliorative Role of Carvone

In Vitro Effect of Cypermethrin on the Perturbations of Rat Erythrocytes: The Ameliorative Role of Carvone

Effect of a mixture of pyrethroids at doses similar to human exposure through food in the Indian context

Novel Mechanism and Kinetics of Tetramethrin Degradation Using an Indigenous Gordonia cholesterolivorans A16

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