Indoles and Related Ehrlich Reactors

Jepson, J.B.

doi:10.1016/b978-0-433-30503-3.50016-x

Cited by 14 publications

(15 citation statements)

References 80 publications

(6 reference statements)

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“…AHT was extracted as described by McCorkindale et al (1983), and assayed using the acid-diazotized sulphanilic acid test for 6-hydroxyindoles (Jepson, 1960;Jepson et al, 1962). A 0.1 ml volume of a 5 % (w/v) aqueous solution of NaNO, was added to 0.1 g sulphanilic acid dissolved in 1 ml concentrated HCl, and left for 5 min on ice.…”

Section: Methodsmentioning

confidence: 99%

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The genetics of conidiophore pigmentation in Aspergillus nidulans.

Clutterbuck

1990

Journal of General Microbiology

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The grey-brown pigmentation of Aspergillus nidulans conidiophores depends on the functions of two 'ivory' loci. iuoB codes for a developmental specific phenol oxidase, and mutants accumulate its substrate N-acetyl-6-hydroxytryptophan. ivoA mutants are unable to make this substrate. ygA mutants are also poorly pigmented, and extracts require copper salts to activate both the phenol oxidase and conidial laccase. ivoA and ittoB mutants partially suppress the spore colour phenotype of ygA mutants. Comparisons of morphology, phenol oxidase and substrate accumulation in morphological mutants at the brfA locus suggest that the brfA protein regulates iuoA, ivoB and morphogenetic loci independently. The medA locus, which also affects morphology and pigmentation, may code for a modifier of brlA function. abaA mutants which are blocked at a later stage of development than brfA or medA mutants have low phenol oxidase levels, implying that by this stage of development the activity of the ivoB locus is declining.

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Section: Methodsmentioning

confidence: 99%

“…AHT and other indoles were detected by paper chromatography of methanolic extracts of dried mycelium (Jepson, 1960), using butanol/ acetic acid/water (60 : 15 : 25 by vol.) solvent and stained with Ehrlich's reagent or diazotized sulphanilic acid.…”

Section: Methodsmentioning

confidence: 99%

The genetics of conidiophore pigmentation in Aspergillus nidulans.

Clutterbuck

1990

Journal of General Microbiology

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“…The inhibitors and accelerators lociated by bioassay proceduires did niot give color reactions with the FEhrlich rcagenit and were thtus considered to be non-indolic. Tests wi'th xantthyd'rol, diazotized sul fanilic acid, and other location relagents (9,16) Alkaline Ether Fraction. The solubi.lity fraction selecited for chromatogra'phi'c separation con'tained all of the major and minor alka.loids and phenolic (4) showed the deveilopment of paltches o'f necrotic cells in roots of corn.…”

Section: Resultsmentioning

confidence: 99%

“…In some experiments the acid aqueous layer was broulghit to pH 7.5 to 8.0 with sollicl sodium bicarbonate and again extracted w,ilth ether. The A modified Ehrlich ireageniit (9) was the primary color test used for locating iindaoles in all of this work.…”

Section: Methodsmentioning

confidence: 99%

Growth Substances From Veratrum tenuipetalum

Olney

1968

Plant Physiol.

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A bstract. Young leaves and buds of Veratrum tenuipetalum yielded non-indolic growth accelerators and inhibitors in the acidic ether fraction. The titer of accelerators decreased while the inhibitors increased as leaves matured. This was also true when comparing extracts of immature and fruiting inflorescences. Indole 3-acetic acid was at no time detected in leaf, bud, and inflorescence extracts but indole 3-acetyl-aspartic acid was readily recovered from all of these.The alkaline ether fraction of leaf base meristems, buds, roots, and rhizomes was rich in crystalline and amorphous alkaloids and phenolic acids. Reducing the quantity of major substances through crystallization, precipitation, and filtration permitted thin film chromatographic separation of the minor alkaloids and phenolic acids in the presence of the major ones. The unchromatographed mixture, and also certain of the purified major and minor alkaloids studied, strongly inhibited growth in germinating oat and winter rye seeds. In addition, profound changes in morphology and cytology of the seminal roots resulted. DNA disappeared partially to completely from affected tissue in 2 day germinated seeds. I No. 3 ch 1968Western Veratrutin species have a reputationi for toxicity to cattile and sheep (1). \VThen I observed in the Colorado Rockies massive monospecific stands of Veratrutm and learned that these had develloped over the pa,sgt 25 years through ecological invasiveness this suiggested tha't substanices capable of influencing germination, morphogenesis, andcl growvth might be isolatedl from this gentus. At the periphery of these stands species of Delphinimn and Aconitunm and even smal,l subalpine fir or Englemann spruice trees ha(l a sp ndily anid pale look. Cou,ld th,is be expla;ined oni the basis of Veratruwlt root-secreted products or snibstanices liberated to the soil by frost kill of the aboove gro-und parts? I determined to investigate all parts of the plant.

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“…This was done as described by Jepson (1960) and Jepson et al (1962). One ml of sulphanilic acid reagent (0-9 g sulphanilic acid in 9 ml concentrated hydrochloric acid, diluted 1/10 with water) was added to 0.1 ml 5% aqueous sodium nitrite, and left on ice for 5 min.…”

Section: Metal Analysismentioning

confidence: 99%

N-Acetyl-6-hydroxytryptophan oxidase, a developmentally controlled phenol oxidase from Aspergillus nidulans

Birse

Clutterbuck

1990

Journal of General Microbiology

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We have purified a specific phenol oxidase which is produced during conidiophore development in the fungus AspergiZZus niduZms. Two active forms (A and B) have molecular masses of 50 and 48 kDa respectively; they have identical N-termini (24 residues). We have analysed the metal ion content of the B form; it is unusual in consisting of one zinc and two copper atoms per molecule. A temperature-sensitive mutant (ivoB192) produces a thermolabile enzyme, implying that ivoB is the structural locus. The natural substrate of the enzyme is N-acetyl-6-hydroxytryptophan, but it can be assayed colorimetrically or polarographically using hydroquinone monomethyl ether (HME) as substrate. It will also oxidize p-cresol, but not tyrosine, 3,4-dihydroxyphenylalanine or o-methoxyphenol. Colour development with HME substrate is strongly enhanced by high ammonium ion concentrations. Activity against HME is inhibited by 2,3-dihydroxynaphthalene, phenylhydrazine, diethyl dithiocarbamate and &hydroxyquinolene.

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Indoles and Related Ehrlich Reactors

Cited by 14 publications

References 80 publications

The genetics of conidiophore pigmentation in Aspergillus nidulans.

The genetics of conidiophore pigmentation in Aspergillus nidulans.

Growth Substances From Veratrum tenuipetalum

N-Acetyl-6-hydroxytryptophan oxidase, a developmentally controlled phenol oxidase from Aspergillus nidulans

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