Abstract:Indole 3-acetic acid (IAA) is the principal hormone which regulates various developmental and physiological processes in plants. IAA production is considered as a key trait for supporting plant growth. Hence, in this study, production of indole-3-acetic acid (IAA) by a basidiomycetous red yeast Rhodosporidium paludigenum DMKU-RP301 (AB920314) was investigated and improved by the optimization of the culture medium and culture conditions using one factor at a time (OFAT) and response surface methodology (RSM). T… Show more
“…Some concerns exist that this concentration does not reflect all actual concentrations found in the wild or those produced by microbes. Many studies have shown that the amount of IAA that many bacteria and fungi can secrete was similar to or even more than 500 µM which is the highest dose we used here (Mohite, 2013;Limtong et al, 2014;Nutaratat et al, 2015;Nutaratat, Monprasit & Srisuk, 2017). According to our previous study, some fungi (e.g., Aureobasidium pullulans) can even can secrete more than 200 µg/mL IAA (∼1,000 µM) (Fu et al, 2016).…”
Green microalgae of the genus Desmodesmus are characterized by a high degree of phenotypic plasticity (i.e. colony morphology), allowing them to be truly cosmopolitan and withstand environmental fluctuations. This flexibility enables Desmodesmus to produce a phenotype–environment match across a range of environments broader compared to algae with more fixed phenotypes. Indoles and their derivatives are a well-known crucial class of heterocyclic compounds and are widespread in different species of plants, animals, and microorganisms. Indole-3-acetic acid (IAA) is the most common, naturally occurring plant hormone of the auxin class. IAA may behave as a signaling molecule in microorganisms, and the physiological cues of IAA may also trigger phenotypic plasticity responses in Desmodesmus. In this study, we demonstrated that the changes in colonial morphs (cells per coenobium) of five species of the green alga Desmodesmus were specific to IAA but not to the chemically more stable synthetic auxins, naphthalene-1-acetic acid and 2,4-dichlorophenoxyacetic acid. Moreover, inhibitors of auxin biosynthesis and polar auxin transport inhibited cell division. Notably, different algal species (even different intraspecific strains) exhibited phenotypic plasticity different to that correlated to IAA. Thus, the plasticity involving individual-level heterogeneity in morphological characteristics may be crucial for microalgae to adapt to changing or novel conditions, and IAA treatment potentially increases the tolerance of Desmodesmus algae to several stress conditions. In summary, our results provide circumstantial evidence for the hypothesized role of IAA as a diffusible signal in the communication between the microalga and microorganisms. This information is crucial for elucidation of the role of plant hormones in plankton ecology.
“…Some concerns exist that this concentration does not reflect all actual concentrations found in the wild or those produced by microbes. Many studies have shown that the amount of IAA that many bacteria and fungi can secrete was similar to or even more than 500 µM which is the highest dose we used here (Mohite, 2013;Limtong et al, 2014;Nutaratat et al, 2015;Nutaratat, Monprasit & Srisuk, 2017). According to our previous study, some fungi (e.g., Aureobasidium pullulans) can even can secrete more than 200 µg/mL IAA (∼1,000 µM) (Fu et al, 2016).…”
Green microalgae of the genus Desmodesmus are characterized by a high degree of phenotypic plasticity (i.e. colony morphology), allowing them to be truly cosmopolitan and withstand environmental fluctuations. This flexibility enables Desmodesmus to produce a phenotype–environment match across a range of environments broader compared to algae with more fixed phenotypes. Indoles and their derivatives are a well-known crucial class of heterocyclic compounds and are widespread in different species of plants, animals, and microorganisms. Indole-3-acetic acid (IAA) is the most common, naturally occurring plant hormone of the auxin class. IAA may behave as a signaling molecule in microorganisms, and the physiological cues of IAA may also trigger phenotypic plasticity responses in Desmodesmus. In this study, we demonstrated that the changes in colonial morphs (cells per coenobium) of five species of the green alga Desmodesmus were specific to IAA but not to the chemically more stable synthetic auxins, naphthalene-1-acetic acid and 2,4-dichlorophenoxyacetic acid. Moreover, inhibitors of auxin biosynthesis and polar auxin transport inhibited cell division. Notably, different algal species (even different intraspecific strains) exhibited phenotypic plasticity different to that correlated to IAA. Thus, the plasticity involving individual-level heterogeneity in morphological characteristics may be crucial for microalgae to adapt to changing or novel conditions, and IAA treatment potentially increases the tolerance of Desmodesmus algae to several stress conditions. In summary, our results provide circumstantial evidence for the hypothesized role of IAA as a diffusible signal in the communication between the microalga and microorganisms. This information is crucial for elucidation of the role of plant hormones in plankton ecology.
“…4, a high level of IAA (490 mg/L) was obtained when mannitol was added as the carbon source. Many researchers reported that carbon sources addition in culture medium increased IAA synthesis (Datta and Basu 2000; Nutaratat et al 2015). Fig.…”
Indole acetic acid (IAA) is a plant growth-promoting hormone used in agriculture; therefore, its continuous production is of paramount importance. IAA-producing eight bacteria were isolated from the rhizosphere of Verbascum vulcanicum. Among them, Arthrobacter agilis A17 gave maximum IAA production (75 mg/L) and this strain was used to immobilization studies. The A. agilis A17 cells were immobilized in calcium alginate for the production of IAA. Optimization of process parameters for IAA production was carried out to enhance IAA production using immobilized cells. The maximal production of IAA was 520 mg/L under the following optimal conditions: 1% mannitol, 30 °C, pH 8.0, and 24 h incubation. It was determined that the immobilized cells could be reused (13 times) for the production of IAA.
“…In recent years, yeasts isolated from various natural habitats have been found to havepotential as effective producers of various biotechnological products worldwide. Various wild yeast species have been reported to have a vital role in modern industrial biotechnology, for example, for the production of citric acid [51], single cell proteins [52,53], ethanol [54,55], microbial oils [56][57][58], indole-3-acetic acid (IAA) [59], itaconic acid [60], glycolipid biosurfactants [49], arabitol [61], xylitol [62], erythritol and mannitol [63]. In this study, we isolated 217 yeast strains of 10 species in the Ascomycota and 35 species in the Basidiomycota.…”
Section: Discussionmentioning
confidence: 99%
“…Papiliotrema flavescens has been reported to be able to produce αgalactosidase [65]. The strains of R. paludigena and H. sinensis also showed the ability to produce large qualities of indole-3-acetic acid (IAA) [59]. D. churashimaensis and M. antarcticus, which previously belonged to the genus Pseudozyma, have been applied for itaconic acid production [60].…”
The ecology and diversity of phylloplane yeasts is less well understood in tropical regions than in temperate ones. Therefore, we investigated the yeast diversity associated with the phylloplane of corn, an economically important crop in Thailand, by a culture-dependent method. Thirty-six leaf samples were collected and 217 yeast strains were isolated by plating leaf-washings. The strains were grouped by PCR-fingerprinting and representative strains were identified by analysis of the D1/D2 domain of the large subunit rRNA gene. In total, 212 strains were identified within 10 species in the Ascomycota and 32 species in the Basidiomycota. Five strains represented potential new species in the Basidiomycota, one strain was recently described as Papiliotrema plantarum, and four strains belonged to the genera Vishniacozyma and Rhodotorula. A higher number of strains in the Basidiomycota (81.6%) was obtained. Hannaella sinensis was the species with the highest occurrence. Principal coordinates analysis ordinations of yeast communities revealed that there were no differences in the similarity of the sampling sites. The estimation of the expected species richness showed that the observed species richness was lower than expected. This work indicated that a majority of yeast associated with the phylloplane of corn plant belongs to the phylum Basidiomycota.
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