Indole-3-Acetic Acid, Polyamines, and Phenols in Hardwood Cuttings of Recalcitrant-to-Root Wild Grapes Native to East Asia:<i>Vitis davidii</i>and<i>Vitis kiusiana</i>

Shiozaki, Shuji; Makibuchi, Masahiro; Ogata, Takanari

doi:10.1155/2013/819531

Cited by 10 publications

(7 citation statements)

References 32 publications

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“…However, adventitious roots are di cult to be induced in many grape species or varieties with important breeding utilization, such as V. davidii, V. quinquangularis (syn. V. kiusiana), and V. amurensis which are wild grapes native to East Asia (Shiozaki et al 2013). The V. rotundifolia (Muscadine) (Castro et al 1994), V. champinii (Saleh 2019), V. aestivalis (Keeley et al 2004), V. berlandieri (Kracke et al 1981;Smart et al 2003), and some cultivars of V. vinifera are also known to be di cult to root from their woody cuttings.…”

Section: Introductionmentioning

confidence: 99%

A simple, rapid, and quantifiable system for studying adventitious root formation in grapevine

et al. 2022

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Woody cutting is customarily utilized as material in research of grape adventitious root formation (ARF).However, phenotypic heterogeneity caused by the complex background in uenced its use for molecular mechanism research of ARF of grape. The present study tested various types of explants from grape tissue culture plantlets and found the whole leaf: blade with petiole (LP) was the simplest unit that can easily form adventitious root (AR). LP explants which can be easily obtained, directly generate ARs via de novo organogenesis from the base of the petiole. The plantlet age, node position, blade size, the health condition of leaves, and light intensity have been demonstrated to affect the homogeneity of ARF phenotype in LP. By controlling these parameters, selected LPs cultured on medium with 6 g•L -1 agar and 10 g•L -1 sucrose under dark conditions started rooting at 6-7 day after culture (DAC) and reached 100% rooting rate within 13-14 DAC. Using this system, the core role of auxin on ARF was veri ed by exogenous application of indole butyric acid (IBA) and N-1-naphthylphthalamic acid (NPA). Strikingly, we found light promoted ARF in the absence of sucrose, but inhibited ARF in the presence of sucrose (10 g•L -1 ), while a low concentration of 0.1 mg•L -1 NPA partially relieved the inhibition. Finally, this study con rmed exogenous plant growth regulators (PGRs), including 6-benzyl aminopurine (6-BA), gibberellic acid 3 (GA 3 ), and 2,4-Epibrassinolide (EBR) inhibited ARF signi cantly. This simple, rapid, quanti able ARF research system provides a new approach to study the factors in uencing the formation and development of grape adventitious roots and establishes a framework for investigating the mechanism of grape adventitious root induction and initiation.

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Section: Introductionmentioning

confidence: 99%

A simple, rapid, and quantifiable system for studying adventitious root formation in grapevine

et al. 2022

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“…[ 26 ] or Vitis vinifera [ 27 ], we found no correlation between callus size and AR number, suggesting that these two processes (callus growth and establishment of AR primordia) could be regulated independently in Prunus rootstocks. Indeed, studies conducted on grape rootstocks also confirmed the independent regulation of callus and AR formation in this species [ 28 ]. The rooting of hardwood cuttings from Garnem and GF 677 differed mainly in the timing of AR formation, resulting in a significantly higher number of ARs in Garnem.…”

Section: Discussionmentioning

confidence: 79%

Effects of Auxin (Indole-3-butyric Acid) on Adventitious Root Formation in Peach-Based Prunus Rootstocks

Justamante

Mhimdi

Molina-Pérez

et al. 2022

Plants

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Several Prunus species are among the most important cultivated stone fruits in the Mediterranean region, and there is an urgent need to obtain rootstocks with specific adaptations to challenging environmental conditions. The development of adventitious roots (ARs) is an evolutionary mechanism of high relevance for stress tolerance, which has led to the development of environmentally resilient plants. As a first step towards understanding the genetic determinants involved in AR formation in Prunus sp., we evaluated the rooting of hardwood cuttings from five Prunus rootstocks (Adafuel, Adarcias, Cadaman, Garnem, and GF 677) grown in hydroponics. We found that auxin-induced callus and rooting responses were strongly genotype-dependent. To investigate the molecular mechanisms involved in these differential responses, we performed a time-series study of AR formation in two rootstocks with contrasting rooting performance, Garnem and GF 677, by culturing in vitro microcuttings with and without auxin treatment (0.9 mg/L of indole-3-butyric acid [IBA]). Despite showing a similar histological structure, Garnem and GF677 rootstocks displayed dynamic changes in endogenous hormone homeostasis involving metabolites such as indole-3-acetic acid (IAA) conjugated to aspartic acid (IAA-Asp), and these changes could explain the differences observed during rooting.

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“…On the other hand, Somkuwar et al (2011) reported that high levels of phenols in grape rootstock cuttings at planting may be correlated with the formation of root primordia and stimulate the elongation of the roots. With respect to the phenols type, some phenolic compounds had a positive effect such as phloroglucinol and chlorogenic acid on rooting (Wilson and Van Staden, 1990, Wang 1991and Shiozaki et al, 2013. Also, gallocatechin found in ‗Campbell Early' cuttings which may be prevent IAA oxidation.…”

Section: Discussionmentioning

confidence: 99%

Effect of Pre-Grafting Incubation and Grafted Cuttings Positionon Grape Grafting Success

2016

NIDOC-ASRT

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HIS EXPREMENTS was carried out during two successive ……..seasons 2015 and 2016 at the nursery of pomology department, Faculty of Agriculture, Cairo university, Egypt. The study was aimed to investigate the effect of two fators namely i.e, pre-grafting incubation and grafting materials position during callusing on grafting success of flame seedless grapes onto Salt Creek rootstock. Callusing was carried out in three positions i.e, horizontal, upright and upright down either with or without pre-grafting incubation. The results showed that either pre-grafting incubation or callusing at uprightposition increased significantly callus percent and degree, sucker number, rooting rate, bud break percent during callusing period and grafting success percent. The interaction, betweentreatments revealed thatcallusing at uprightpositionaccompanied with pregrafting incubation gave the best results in this respect. It can be concluded that callusing at uprightposition either with or without pregrafting incubation increased grafting success significantly compared to callusing at horizontal or upright down position.Chemically, higher grafting success is accompanied with lower phenols, reducing sugars and total indoles. It may be concluded that there is no antagonistic effect between root and callusformation in grafted cuttings. Also, upright position for incubation the grafted cuttings are better than pregrafted incubation in increasing grape grafted cuttings.

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Indole-3-Acetic Acid, Polyamines, and Phenols in Hardwood Cuttings of Recalcitrant-to-Root Wild Grapes Native to East Asia:Vitis davidiiandVitis kiusiana

Cited by 10 publications

References 32 publications

A simple, rapid, and quantifiable system for studying adventitious root formation in grapevine

A simple, rapid, and quantifiable system for studying adventitious root formation in grapevine

Effects of Auxin (Indole-3-butyric Acid) on Adventitious Root Formation in Peach-Based Prunus Rootstocks

Effect of Pre-Grafting Incubation and Grafted Cuttings Positionon Grape Grafting Success

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