Indocyanine-green fluorescence guided anatomical segmentectomy for HCC with portal thrombosis: the counter-fluorescence technique

Pansa, Andrea; Torzilli, Guido; Procopio, Fabio; Fabbro, Daniele Del

doi:10.1007/s13304-019-00695-4

Cited by 8 publications

(2 citation statements)

References 10 publications

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“…When combined with preoperative 3D reconstruction and IOUS, it can be solid real-time navigation tool to make a huge difference in accuracy of liver resections. However, best time point and dose of ICG administration for tumor identification and liver segmentation remains unclear due to various patient backgrounds and imaging settings in the previous studies 56–101 . Thus, its administration strongly needs to be tailored case by case in daily practice.…”

Section: Discussionmentioning

confidence: 99%

Indocyanine Green Fluorescence Navigation in Liver Surgery

et al. 2022

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Background:Indocyanine green (ICG) fluorescence has proven to be a high potential navigation tool during liver surgery; however, its optimal usage is still far from being standardized.Methods:A systematic review was conducted on MEDLINE/PubMed for English articles that contained the information of dose and timing of ICG administration until February 2021. Successful rates of tumor detection and liver segmentation, as well as tumor/patient background and imaging settings were also reviewed. The quality assessment of the articles was performed in accordance with the Scottish Intercollegiate Guidelines Network (SIGN).Results:Out of initial 311 articles, a total of 72 manuscripts were obtained. The quality assessment of the included studies revealed usually low; only 9 articles got qualified as high quality. Forty articles (55%) focused on open resections, whereas 32 articles (45%) on laparoscopic and robotic liver resections. Thirty-four articles (47%) described tumor detection ability, and 25 articles (35%) did liver segmentation ability, and the others (18%) did both abilities. Negative staining was reported (42%) more than positive staining (32%). For tumor detection, majority used the dose of 0.5 mg/kg within 14 days before the operation day, and an additional administration (0.02–0.5 mg/kg) in case of longer preoperative interval. Tumor detection rate was reported to be 87.4% (range, 43%–100%) with false positive rate reported to be 10.5% (range, 0%–31.3%). For negative staining method, the majority used 2.5 mg/body, ranging from 0.025 to 25 mg/body. For positive staining method, the majority used 0.25 mg/body, ranging from 0.025 to 12.5 mg/body. Successful segmentation rate was 88.0% (range, 53%–100%).Conclusion:The time point and dose of ICG administration strongly needs to be tailored case by case in daily practice, due to various tumor/patient backgrounds and imaging settings.

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Section: Discussionmentioning

confidence: 99%

Indocyanine Green Fluorescence Navigation in Liver Surgery

et al. 2022

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“…As a relatively nontoxic protein-bound compound, ICG is taken up by some cells [ 25 , 26 ]. The detailed mechanisms for intracellular localization of ICG have not been fully clarified.…”

Section: Discussionmentioning

confidence: 99%

Interventional Oncolytic Immunotherapy with LTX-315 for Residual Tumor after Incomplete Radiofrequency Ablation of Liver Cancer

Zhou

Kan

Zhang

et al. 2022

Cancers

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Objective: To investigate the feasibility of interventional oncolytic immunotherapy with LTX-315 for residual tumors after incomplete radiofrequency ablation (iRFA) of VX2 liver tumors in a rabbit model. Methods: For in vitro experiments, VX2 tumor cells were treated with: (1) phosphate buffered saline, (2) radiofrequency hyperthermia (RFH), (3) LTX-315, and (4) RFH plus LTX-315. The residual tumors after iRFA of VX2 liver tumors were treated with: (1) phosphate buffered saline served as control, (2) 2 mg LTX-315, and (3) 4 mg LTX-315. MTS assay, fluorescence microscopy, and flow cytometry were used to compare cell viabilities and apoptosis among different groups. Ultrasound imaging was used to follow up the tumor growth, which were correlated with the optical imaging and subsequent histology. Results: For in vitro experiments, compared with the other three groups, MTS assay demonstrated the lowest cell viability, fluorescence microscopy showed the least survival cells, and apoptosis analysis revealed the highest percentage of apoptosis cells in the combination treatment groups (p < 0.001). For in vivo experiments, ultrasound imaging showed the smallest tumor volume in the group with 4 mg LTX-315 therapy compared with the other two groups (p < 0.001). The optical imaging and histopathological analysis showed complete necrosis of the tumors in the group with 4 mg LTX-315 therapy. A significant increase of CD8+ T cells and HSP70 and a significant decrease of Tregs were observed in residual tumors in the group with 2 mg LTX-315 therapy compared with the control group (p < 0.001). Conclusion: Interventional oncolytic immunotherapy with LTX-315 for residual tumors after iRFA of liver cancer is feasible, which may open up new avenues to prevent residual tumors after RFA of intermediate-to-large liver cancers.

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Glissonean Pedicles for Liver-Sparing Parenchymal Resection Lesson Learned from Open Surgery

Procopio,

Torzilli

2023

Glissonean Pedicles Approach in Minimally Invasive Liver Surgery

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Indocyanine-green fluorescence guided anatomical segmentectomy for HCC with portal thrombosis: the counter-fluorescence technique

Cited by 8 publications

References 10 publications

Indocyanine Green Fluorescence Navigation in Liver Surgery

Indocyanine Green Fluorescence Navigation in Liver Surgery

Interventional Oncolytic Immunotherapy with LTX-315 for Residual Tumor after Incomplete Radiofrequency Ablation of Liver Cancer

Glissonean Pedicles for Liver-Sparing Parenchymal Resection Lesson Learned from Open Surgery

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