Indocyanine Green-Based Theranostic Nanoplatform for NIR Fluorescence Image-Guided Chemo/Photothermal Therapy of Cervical Cancer

Ma, Rong; Alifu, Nuernisha; Du, Zhimin; Chen, Shuang; Heng, Youqiang; Wang, Jing; Zhu, Lijun; Ma, Cailing; Zhang, Xueliang

doi:10.2147/ijn.s318678

Cited by 36 publications

(24 citation statements)

References 54 publications

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“…And it was found that the fluorescence was optimal for NIR imaging at 5 mg/kg to 10 mg/kg for 24 h ( Jiang et al, 2015 ). 10 mg/kg ICG was applied for NIR fluorescence imaging of cervical cancer for 48 h ( Ma et al, 2021 ). Therefore, a dose of 10 mg/kg ICG was chosen in our in vivo PA imaging for 48 h. As shown in Figure 5A , in the CMI group, high-fluorescence signals were easily observed in the tumor sites.…”

Section: Resultsmentioning

confidence: 99%

“…In PA imaging, the mice were anesthetized with 5% isoflurane. Then, free ICG, MI, and CMI were intravenously injected via the tail vein with an ICG dose of 10 mg/kg, ( n = 3/group) ( Jiang et al, 2015 ; Ma et al, 2021 ). The real-time tumor PA imaging and PA signal of free ICG and ICG formulations were recorded at 48 h postinjection using the Vevo LAZR-X multimode imaging system.…”

Section: Methodsmentioning

confidence: 99%

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Efficient Photoacoustic Imaging With Biomimetic Mesoporous Silica-Based Nanoparticles

Huang

Guan

Lin

et al. 2021

Front. Bioeng. Biotechnol.

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Indocyanine green (ICG), a near-infrared (NIR) fluorescent dye approved by the Food and Drug Administration (FDA), has been extensively used as a photoacoustic (PA) probe for PA imaging. However, its practical application is limited by poor photostability in water, rapid body clearance, and non-specificity. Herein, we fabricated a novel biomimetic nanoprobe by coating ICG-loaded mesoporous silica nanoparticles with the cancer cell membrane (namely, CMI) for PA imaging. This probe exhibited good dispersion, large loading efficiency, good biocompatibility, and homologous targeting ability to Hela cells in vitro. Furthermore, the in vivo and ex vivo PA imaging on Hela tumor-bearing nude mice demonstrated that CMI could accumulate in tumor tissue and display a superior PA imaging efficacy compared with free ICG. All these results demonstrated that CMI might be a promising contrast agent for PA imaging of cervical carcinoma.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Efficient Photoacoustic Imaging With Biomimetic Mesoporous Silica-Based Nanoparticles

Huang

Guan

Lin

et al. 2021

Front. Bioeng. Biotechnol.

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“…In the synthesis of CuS@BSA NPs, BSA was used to improve the water solubility, biocompatibility, and stability of CuS nanomaterials, which made it easier for the nanoparticles to enter the cells. Furthermore, many studies have shown that the encapsulation with BSA can also promote the function of nanoparticles (Zhang et al, 2018;Ma et al, 2021;Radwan et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

CuS@BSA-NB2 Nanoparticles for HER2-Targeted Photothermal Therapy

Ying

Qin

et al. 2022

Front. Pharmacol.

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Breast cancer is characterized by the uncontrolled proliferation of breast epithelial cells under the action of a variety of carcinogens. Although HER2-inhibitors were currently applied for HER2-positive breast cancer patients, they didn’t work for patients with resistance to HER2-targeted anti-cancer drugs. In this work, we prepared novel CuS@BSA-NB2 nanoparticles (NPs) for breast cancer photothermal therapy (PTT). The NPs had good biocompatibility due to the Bovine Serum Albumin (BSA) encapsulating and excellent targeting to HER2 because of nanobody 2 (NB2). Under 808 nm laser irradiation, CuS@BSA-NB2 NPs had high photothermal conversion efficiency and photothermal stability. Meanwhile, we constructed a stable cell line of MDA-MB-231/HER2 with a high expression of HER2 protein. Immunofluorescence and ICP-MS assays showed that CuS@BSA-NB2 NPs can be specifically enriched and be ingested in MDA-MB-231/HER2 cells. Furthermore, CuS@BSA-NB2 NPs had shown a more significant photothermal treatment effect than CuS@BSA under certain treatment conditions for MDA-MB-231/HER2. In addition, the cytotoxicity assay demonstrated that CuS@BSA-NB2 NPs had a low toxicity for MDA-MB-231/HER2 cells. The above results suggested that CuS@BSA-NB2 NPs were great photothermal therapeutic agents to reduce the malignant proliferation of breast epithelial cells and have potential for breast cancer therapy.

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“…For example, Y. Zhang et al synthesized laser-triggered gold nanoparticles with synergistic chemotherapyphotothermal effect for targeted colon cancer therapy [30]. Rong Ma et al constructed a therapeutic indocyanine green-based nanoplatform for near-infrared uorescent image-guided chemotherapy/ photothermal treatment of cervical cancer [31]. J. Huang et al developed metal organic frameworkcoated gold nanorod as an on-demand drug delivery platform for chemo-photothermal therapy [32].…”

Section: Discussionmentioning

confidence: 99%

AS1411-targeted graphene oxide nano-drug delivery system for chemo-photothermal therapy of cervical cancer

Gao

Liang

Che

et al. 2022

Preprint

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The aim of this study is to design a novel pH and photothermal dual-responsive nanodrug delivery system with high biocompatibility and tumor targeting. Based on the high loading rate and good photothermal properties of graphene oxide (GO), we selected GO modified with chitosan (CO) and γ-polyglutamic acid (γ-PGA) as nanocarriers. CO and γ-PGA increased the dispersion of GO and improved the solubility of GO-CO-γ-PGA (G-C-P) in solution. To further improve the targeting of the system, the nucleic acid aptamer NH2-AS1411 (APT), which targets the nucleolin (C23), was attached to G-C-P and the targeted nano-delivery system APT-GO-CO-γ-PGA (A-G-C-P) was prepared by amidation. The synthesized samples were characterized using Fourier transform infrared spectroscopy (FTIR), Ultraviolet-visible spectrophotometer (UV-Vis), X-ray diffraction (XRD), Transmission electron microscopy (TEM), Dynamic light scattering (DLS) and Zeta potential, and their good biocompatibility and stable photothermal conversion properties were demonstrated experimentally. The nanocarrier can act as a photothermal agent, generating high temperatures to induce cell damage. The lower hemolysis rate reflects the good biocompatibility of the carrier. Doxorubicin hydrochloride (DOX) was selected as the model drug with a nanocarrier loading of 37.0 ± 0.74%, showing dual response drug release characteristics of pH and NIR light. The cytotoxicity and photothermal toxicity of the nanocarrier A-G-C-P and the drug delivery system APT-GO-CO-γ-PGA-DOX (A-G-C-P-D) on HeLa cells were investigated in cellular experiments. It was found that A-G-C-P had almost no toxic side effects on the cells, and the killing effect of A-G-C-P-D with the application of near-infrared light irradiation was more obvious, proving its good photothermal therapeutic effect, and further demonstrating that the combined effect of chemotherapy and photothermal treatment is significant than that of single treatment. Confocal microscopy and flow cytometry determined the distribution of DOX in the cells to different degrees after cell internalization. In vivo anti-tumor experiments were conducted to further investigate the therapeutic effects and safety of the targeted nano-drug delivery system in concert with photothermal. Compared with the weight loss and heart tissue damage caused by free DOX, A-G-C-P-D and NIR light irradiation did not cause any tissue damage and toxic side effects in nude mice, and have good biosafety; A-G-C-P-D and NIR light irradiation can inhibit tumor growth, cause damage to tumor tissue, significantly reduce Ki67 expression and increase Caspase-3 expression, all of which prove their good anti-tumor effects. This relatively non-invasive approach may provide a good direction for targeted drug delivery and chemotherapeutic photothermal treatment of tumors, greatly reducing the side effects of chemotherapy. The therapeutic effect of this nano-drug delivery system provides new ideas for clinical treatment strategies with potential applications and reference values, offering a broad prospect for biological applications.

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Indocyanine Green-Based Theranostic Nanoplatform for NIR Fluorescence Image-Guided Chemo/Photothermal Therapy of Cervical Cancer

Cited by 36 publications

References 54 publications

Efficient Photoacoustic Imaging With Biomimetic Mesoporous Silica-Based Nanoparticles

Efficient Photoacoustic Imaging With Biomimetic Mesoporous Silica-Based Nanoparticles

CuS@BSA-NB2 Nanoparticles for HER2-Targeted Photothermal Therapy

AS1411-targeted graphene oxide nano-drug delivery system for chemo-photothermal therapy of cervical cancer

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