CuS@BSA-NB2 Nanoparticles for HER2-Targeted Photothermal Therapy

Abstract

Breast cancer is characterized by the uncontrolled proliferation of breast epithelial cells under the action of a variety of carcinogens. Although HER2-inhibitors were currently applied for HER2-positive breast cancer patients, they didn’t work for patients with resistance to HER2-targeted anti-cancer drugs. In this work, we prepared novel CuS@BSA-NB2 nanoparticles (NPs) for breast cancer photothermal therapy (PTT). The NPs had good biocompatibility due to the Bovine Serum Albumin (BSA) encapsulating and excellent targeting to HER2 because of nanobody 2 (NB2). Under 808 nm laser irradiation, CuS@BSA-NB2 NPs had high photothermal conversion efficiency and photothermal stability. Meanwhile, we constructed a stable cell line of MDA-MB-231/HER2 with a high expression of HER2 protein. Immunofluorescence and ICP-MS assays showed that CuS@BSA-NB2 NPs can be specifically enriched and be ingested in MDA-MB-231/HER2 cells. Furthermore, CuS@BSA-NB2 NPs had shown a more significant photothermal treatment effect than CuS@BSA under certain treatment conditions for MDA-MB-231/HER2. In addition, the cytotoxicity assay demonstrated that CuS@BSA-NB2 NPs had a low toxicity for MDA-MB-231/HER2 cells. The above results suggested that CuS@BSA-NB2 NPs were great photothermal therapeutic agents to reduce the malignant proliferation of breast epithelial cells and have potential for breast cancer therapy.

Novel Twin‐Crystal Nanosheets with MnO2 Modification to Combat Bacterial Biofilm against Periodontal Infections via Multipattern Strategies

“…This might be attributed to the fact that the MnO 2 layer decomposed H 2 O 2 to generate O 2 , leading to improve the local hypoxia microenvironment and affect the catabolism and anabolism pathway of anaerobic bacteria. [ 18 ]…”

“…CuS is an excellent photothermal material that has been wellproven in tumor therapy and antibacterial therapy due to its nearinfrared (NIR) excitation and efficient photothermal conversion performance. [18,19] Considering CuS and MnS have similar lattice structures, [20,21] if the copper sulfide and manganese sulfide can be prepared as twin crystals as different structural domains in the lattice, the Fenton effect of manganese sulfide can be enhanced by the photothermal effect of CuS. Meanwhile, the photothermal property of CuS can be further used to dissipate biofilms, which could be expected to achieve multi-functional application within a single nanocrystal material.…”

Effect of Neoadjuvant Chemotherapy on Angiogenesis and Cell Proliferation of Breast Cancer Evaluated by Dynamic Enhanced Magnetic Resonance Imaging

“…Imaging examination plays an important role in the early diagnosis of breast cancer. With its high soft tissue resolution, breast MRI has become increasingly valuable in the diagnosis of breast lesions [ 1 , 2 ]. At present, studies on quantitative parameters of breast MRI mainly focus on DCE-MRI technology [ 3 ].…”

“…Breast cancer is the uncontrolled proliferation of breast epithelial cells due to the action of multiple carcinogenic factors, which is mainly manifested by axillary lymphadenopathy, nipple discharge, and breast mass [ 1 , 2 ]. Its incidence is as high as 15% and increases year by year, seriously affecting women's lives and mental health [ 3 , 4 ].…”

Photothermal effects of CuS-BSA nanoparticles on H22 hepatoma-bearing mice

“…And after intravenous administration to orthotopic HCC mice, CuS@BSA-RGD NPs markedly accumulated in hepatoma tissue, enabling the high-sensitivity photoacoustic visualization of tumor tissue ( Yan et al, 2019 ). CuS@BSA-NB2 synthesized by covalently linking nanobody (NB2) to BSA had the higher targeting for breast cancer with high expression of human epidermal growth factor receptor-2 (HER2) ( Ying et al, 2022 ). Chen et al (2021) labeled the synthesized BSA@CuS-PEG nanocomposites with Cy5.5, a fluorescent dye, to endow the nanomaterials with optical imaging capabilities ( Chen et al, 2021 ).…”