Individual variations of pH, buffer capacity, and concentrations of calcium and phosphate in unstimulated whole saliva

Larsen, Mona; Jensen, Anne; Madsen, Diana; Pearce, E.I.F.

doi:10.1016/s0003-9969(98)00108-3

Cited by 83 publications

(59 citation statements)

References 15 publications

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“…These results are in accordance to the results reported in literature for pH 13 and rate 14 of saliva at various physical and biological conditions. It was observed that the salivary flow rate was not significantly different in the two groups.…”

Section: Salivary Flow Rate and Phsupporting

confidence: 93%

“…The change of salivary pH is of prime importance as many bacterial species prefer a certain pH for their growth. 13 The decreased flow rate in Group I compared to healthy Group II is not significant. It is known that the salivary flow rate is influenced by various biological and environmental factors including age 15 and various medications.…”

Section: Salivary Flow Rate and Phmentioning

confidence: 80%

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Inhibitory activity of Salvadora persica extracts against oral bacterial strains associated with periodontitis: An in-vitro study

Alireza

Rezaei

Hosein

et al. 2014

Journal of Oral Biology and Craniofacial Research

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Miswak Periodontitis Disc diffusionOral bacteria a b s t r a c t Aims: The use of natural plant extracts in pharmacology, medicine and dental hygiene has found a growing interest in modern scientific research. Salvadora persica is a natural tree whose fibrous branches have been approved by the World Health Organization for oral hygiene. Periodontitis is a highly prevalent adult gingival disease that leads to bone destruction and connective tissue attachment loss. The aim of this research was assessment the antimicrobial activities of methanolic extract of Salvadora persica (miswak) on isolated strains from the oral fluid.Methods: In practical section, 50 female university students (21.4 AE 1 year) participated in the study. Based on examination by a periodontist, they were grouped into (Group I, n ¼ 21) and (Group II, n ¼ 29) i.e. with and without periodontitis respectively. Their un-stimulated saliva samples were obtained in sterile tubes. While three bacterial genera, Staphylococcus, Streptococcus and Lactobacillus were identified in all subjects, Enterococcus and Escherichia were only detected in Group I.Results: A statistically significant difference in colonization levels between the two groups was observed. The effect of methanolic extract of S. persica against oral bacterial strains isolated from saliva was investigated using agar disc diffusion and microdilution methods.Although methanolic extract of S. persica was effective on growth inhibition of all strains, it was significantly more effective on Gram positive bacteria than Gram negative ones.Conclusions: Effective substances present in S. persica extracts, exhibit a broad range of antibacterial activity and affect almost all bacterial species regardless of the Gram-staining nature.

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Section: Salivary Flow Rate and Phsupporting

confidence: 93%

Section: Salivary Flow Rate and Phmentioning

confidence: 80%

Inhibitory activity of Salvadora persica extracts against oral bacterial strains associated with periodontitis: An in-vitro study

Alireza

Rezaei

Hosein

et al. 2014

Journal of Oral Biology and Craniofacial Research

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“…As variações individuais em amostras salivares coletadas semanalmente, sugere que a relação entre prevalência de cárie e parâmetros salivares pode não ser evidente devido, tanto a multifatoriedade da doença cárie, como ao fato que a caracterização da saliva não pode ser feita baseada apenas em uma única análise 21 . A análise de crianças de 7 a 12 anos de idade revelou uma correlação muito baixa entre capacidade tampão e lesões de cárie 22 .…”

Section: Discussionunclassified

Fluxo Salivar e Capacidade Tamponante da Saliva como Indicadores de Susceptibilidade à Doença Cárie

Bretas¹,

Rocha²,

Vieira³

et al. 2008

Pesq Bras Odontoped Clin Integr

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RESUMO DESCRIPTORS DESCRITORESSaliva; Índice CPO-D; Cárie dentária. Saliva; DMF Index; Dental caries. Flow Rate and Buffering Capacity of the Saliva as Indicators of the Susceptibility to Caries DiseaseObjective: To correlate salivary indices with the DMFT/dmft index, which is an indicator of caries prevalence, and the adoption of educational measures for oral health promotion. Method: Saliva samples were collected from 56 children of both genders (28 girls and 28 boys), aged 5 to 7 years at the Santana de Itatiaia Municipal School. Prior to saliva collection, the research project was explained to the parents and/or guardians and their written consent was obtained for enrolment of the children in the study. Saliva was collected by masticatory stimulation. Thereafter, the volume of saliva was recorded by the operator and the salivary flow rate was calculated (mL/5 min) for each participant. The buffering capacity of the saliva of each sample was determined by titration, measuring the volume of 0.1 N lactic acid required for decreasing the salivary pH from 6.9 to 3.7.Results: Among the studied children, 23% presented a salivary flow rate below the mean value. In 62% of the children, the values of the buffering capacity of the saliva classified them as susceptible to caries disease. In the present study, there was a moderate correlation (p=0.79) between the buffering capacity of the saliva and DMFT/dmft, confirming the complex etiology of the caries disease. Conclusion: Flow rate and buffering capacity of saliva should be analyzed together with other factors for determining the cariogenic potential. The findings of this study indicate the need of intervening in order to promote of educational measures of prevention and health promotion. Objetivo: Correlacionar índices salivares com o índice CPO-D/ ceo-d que é indicativo da prevalência da cárie e a adoção de práticas educativas para promoção de saúde bucal.Método: A coleta da saliva foi realizada na Escola Municipal Santana de Itatiaia em crianças de ambos os sexos (28 do sexo feminino e 28 do sexo masculino) com idade variando entre 5 e 7 anos, totalizando 56 participantes. Para a realização da coleta foi realizada uma reunião com os pais e/ou responsáveis pela crianças para esclarecimento do projeto e autorização da participação das crianças. A saliva foi coletada por estímulo pela mastigação. Após esse procedimento, o operador anotou o volume de saliva formada e para cada participante foi calculado o fluxo salivar (ml/5min). A capacidade tampão da saliva de cada amostra foi determinada por Titulometria, medindo-se o volume de ácido lático 0,1 N necessário para abaixar o pH salivar de 6,9 ao pH de 3,7.

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“…The pH change of stimulated saliva is more resistant to diurnal variation. 16 This characteristic of stimulated saliva is desirable for a clinical test because the changing salivary pH parameter is very difficult to eliminate completely. Moreover, it is quite hard to standardize the pH measurement of the resting saliva due to the difficulty in collecting the low flow rate saliva and requiring a long collection time.…”

Section: Discussionmentioning

confidence: 99%

Relationship between quantitative assessments of salivary buffering capacity and ion activity product for hydroxyapatite in relation to cariogenic potential

Aiuchi

Kitasako

Fukuda

et al. 2008

Australian Dental Journal

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Background: The ion activity product for hydroxyapatite (Ip HA ) is a comprehensive parameter reflecting pH, calcium and phosphate ion concentration in saliva which govern the degree of saturation with respect to the dissolving tooth mineral. The aim of this study was to evaluate the relationship between quantitative assessments of salivary buffering capacity and Ip HA in relation to cariogenic potential. Methods: Stimulated whole saliva was collected from 33 patients, and the initial pH of samples was measured using a handheld pH meter. Then samples were titrated with 0.1 N HCl to evaluate buffering capacities and divided into three groups (high, medium and low). After measuring concentrations of calcium and phosphate ions in the samples, Ip HA was calculated using the values of the ion concentrations and pH. Differences in the mean pH values, the concentrations of calcium, phosphate ions and log[Ip HA ] among three groups were analysed using the Kruskal Wallis and the Mann-Whitney nonparametric test, p < 0.05. Results: After HCl 50 lL titration, there were statistical differences of the mean pH and Ip HA among each buffering capacity group. Moreover, after 50 lL HCl titration, there was an excellent correlation between the buffer capacity and log[Ip HA ]. Conclusions: The pH change for saliva after HCl titration has a significant influence on the rate of Ip HA .

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Individual variations of pH, buffer capacity, and concentrations of calcium and phosphate in unstimulated whole saliva

Cited by 83 publications

References 15 publications

Inhibitory activity of Salvadora persica extracts against oral bacterial strains associated with periodontitis: An in-vitro study

Inhibitory activity of Salvadora persica extracts against oral bacterial strains associated with periodontitis: An in-vitro study

Fluxo Salivar e Capacidade Tamponante da Saliva como Indicadores de Susceptibilidade à Doença Cárie

Relationship between quantitative assessments of salivary buffering capacity and ion activity product for hydroxyapatite in relation to cariogenic potential

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