Individual return to Leydig cell function after GnRH-immunization of boars

Claus, R.; Rottner, Silke; Rueckert, Christine

doi:10.1016/j.vaccine.2008.05.085

Cited by 35 publications

(30 citation statements)

References 42 publications

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“…Similar studies have also shown that successful immunological castration reduced testosterone to very low levels [Oonk et al 1998;Zamaratskaia et al 2008;Claus et al 2008]. The morphological evaluation of testes of MBP-GnRH-I6 treated boars revealed extreme testes atrophy and disorganized spermatogenesis which is consistent with the published reports of others [Oonk et al 1998;Hilbe et al 2006].…”

Section: Discussionsupporting

confidence: 88%

Immunogenicity of Recombinant Maltose-binding Protein (MBP)–Gonadotropin Releasing Hormone I (GnRH-I)

Fang

Liu

et al. 2010

Systems Biology in Reproductive Medicine

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A recombinant fusion protein, maltose-binding protein (MBP) -gonadotropin releasing hormone I (GnRH-I), was produced by cloning a gene fragment encoding a tandem repeated GnRH-I hexmer peptide (GnRH-I6) into the pMAL-c4x vector which was subsequently expressed in E. coli TB1. MBPGnRH-I6 was affinity purified. MBP-GnRH-I6 was verified by SDS-PAGE and Western blot and immunogenicity tested in boars. Injection of the recombinant fusion protein into boars yielded a high-titer antibody specific for GnRH-I. This was followed by serum testosterone and the degeneration of spermatogenesis. These results showed that MBP-GnRH-I6 acted as a strong immunogen and could be a candidate for an immune antifertility vaccine.

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Section: Discussionsupporting

confidence: 88%

Immunogenicity of Recombinant Maltose-binding Protein (MBP)–Gonadotropin Releasing Hormone I (GnRH-I)

Fang

Liu

et al. 2010

Systems Biology in Reproductive Medicine

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“…The reason is that the time of puberty onset varies with pig breed. Generally, the time of puberty onset in Chinese local pig breed is earlier than the exotic breed, so the first immunisation time in our study was earlier than the other reports (Zamaratskaia et al, 2008;Claus et al, 2008).…”

Section: Discussioncontrasting

confidence: 48%

“…Several other studies have also demonstrated that successful immunological castration reduced testosterone levels to a very low level (Oonk et al, 1998;Zamaratskaia et al, 2008;Claus et al, 2008;Bilskis et al, 2012). In the current study, the first immunisation took place at the age of 9 weeks, which is rather different from procedures with other products (Meloen et al, 1994;Zamaratskaia et al, 2008;Claus et al, 2008). The reason is that the time of puberty onset varies with pig breed.…”

Section: Discussionmentioning

confidence: 94%

Reduced Concentration of Androstenone and Up-Regulation of 3β-Hydroxysteroid Dehydrogenase and 17β-Hydroxysteroid Dehydrogenase mRNA Levels by Active Immunisation Against Gonadotropin Releasing Hormone I

Liu¹,

Fang²,

Jiang³

et al. 2014

Italian Journal of Animal Science

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The aim of this study was to investigate the efficacy of active immunisation against recombinant maltose binding protein-gonadotropin releasing hormone I (MBP-GnRH-I6) in preventing boar taint. The concentrations of testosterone and anti-GnRH-I antibodies in serum were determined by radioimmunoassay and enzyme linked immunosorbent assay, respectively. The mRNA levels of 3 -hydroxysteroid dehydrogenase (3 -HSD) and 17 -hydroxysteroid dehydrogenase (17 -HSD) in testes were analysed by real time quantitative PCR. The concentrations of androstenone and 3-methylindole in backfat were assayed by HPLC. Active immunisation against MBP-GnRH-I6 increased the serum concentration of anti-GnRH-I antibodies (P<0.05) and the levels of 3 -HSD and 17 -HSD mRNA in testis. However, it reduced the serum concentrations of testosterone (P<0.05) and androstenone. The concentration of 3-methylindole in backfat (P>0.05) was not changed. Thus, we conclude that vaccination against MBPGnRH-I6 is a practical and effective method to suppress the synthesis of androstenone.

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“…For the said time increase to be possible, the long-term effects of the Improvac® vaccination should be studied in females, as they have been studied in males (Einarsson, Andersson, Wallgren, Lundström, & Rodriguez-Martinez, 2009;Zamaratskaia et al, 2008), where success of the vaccination has been reported up to 22 weeks after the booster injection (protocol of two doses). Nevertheless, variable resumption of testicular function between 10 and 24 weeks has been reported (Claus, Rottner, & Rueckert, 2008) according to a similar protocol. On the other side, in females, Hernández- have developed a successful long-term pre-pubertal protocol that includes three doses.…”

Section: Introductionmentioning

confidence: 93%

Immune-spaying as an alternative to surgical spaying in Iberian×Duroc females: Effect on quality characteristics and fatty acid profile in dry-cured shoulders and loins

Gamero-Negrón¹,

Pulgar²,

Garcı́a³

2015

Meat Science

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Individual return to Leydig cell function after GnRH-immunization of boars

Cited by 35 publications

References 42 publications

Immunogenicity of Recombinant Maltose-binding Protein (MBP)–Gonadotropin Releasing Hormone I (GnRH-I)

Immunogenicity of Recombinant Maltose-binding Protein (MBP)–Gonadotropin Releasing Hormone I (GnRH-I)

Reduced Concentration of Androstenone and Up-Regulation of 3β-Hydroxysteroid Dehydrogenase and 17β-Hydroxysteroid Dehydrogenase mRNA Levels by Active Immunisation Against Gonadotropin Releasing Hormone I

Immune-spaying as an alternative to surgical spaying in Iberian×Duroc females: Effect on quality characteristics and fatty acid profile in dry-cured shoulders and loins

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