Immunogenicity of Recombinant Maltose-binding Protein (MBP)–Gonadotropin Releasing Hormone I (GnRH-I)

Fang, Fugui; Liu, Ya; Pu, Yong; Lin, Wei; SuoLu, Wang; Zhang, Xiaorong

doi:10.3109/19396368.2010.481005

Cited by 7 publications

(8 citation statements)

References 29 publications

(32 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fang et al [10] used recombinant DNA technology to form maltose binding protein–gonadotropin releasing hormone I (MBP–GnRH-I6) vaccines, which had success in affecting the reproductive systems of pigs [11]. The objective of the present study was to evaluate the ultrastructure of gonadotrophin cells in MBP–GnRH-I6 immunized pigs.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Immunization against recombinant GnRH-I alters ultrastructure of gonadotropin cell in an experimental boar model

Fang

Liu

et al. 2013

Reprod Biol Endocrinol

Self Cite

View full text Add to dashboard Cite

BackgroundGonadotropin cell is the main responsible for the secretion of follicle stimulating hormone (FSH) and luteinizing hormone (LH), and immunocastration reduces the concentrations of serum FSH and LH. A few studies have reported the histological structure of gonadotropin cells obtained from immunocastration animals at the light microscopy level. However, the ultrastructure of gonadotropin cells remains largely unexplored. The aim of this study was to evaluate and to compare ultrastructure of gonadotropin cell in gonadally intact boars and immunologically castrated male animals.FindingsIn this study, serum and adenohypophysis tissue were collected from nine gonadally intact boars and nine male pigs treated with recombinant gonadotropin releasing hormone I (GnRH-I). Anti-GnRH-I antibodies in serum and the ultrastructure of gonadotropin cell in adenohypophysis were determined by enzymelinked immunosorbent assay and electron microscopy, respectively. The results demonstrated that active immunization against recombinant GnRH-I increased serum GnRH-I antibody levels (P<0.05). Ultramicroscopic analysis of gonadotropin cell revealed a decrease (P<0.05) in the number and size of the large granules and small granules in the recombinant GnRH-I immunized animals.ConclusionsWe conclude that immunization against recombinant GnRH-I induces severe atrophy of granules in gonadotropin cell of boars, possibly reflecting GnRH-I regulation of gonadotropin cell.

show abstract

Section: Resultsmentioning

confidence: 99%

“…MBP-GnRH-I6 was prepared and using recombinant DNA techniques as has been previously described [10]. Nine milligrams of MBP-GnRH-I6 or MBP was dissolved in 9 mL phosphate-buffered saline (PBS) and 9 mL of Al(OH) 3 adjuvant (Tianbang, Nanjing, China).…”

Section: Methodsmentioning

confidence: 99%

Immunization against recombinant GnRH-I alters ultrastructure of gonadotropin cell in an experimental boar model

Fang

Liu

et al. 2013

Reprod Biol Endocrinol

Self Cite

View full text Add to dashboard Cite

show abstract

“…For the past 2 decades, GnRH-I sterilization vaccines have been produced by coupling GnRH-I to an antigenic carrier protein using chemical conjugation techniques. In an attempt to overcome this problem, a recombinant fusion protein was developed to serve as a sterilization vaccine in our laboratory (Fang et al, 2009(Fang et al, , 2010b. In an attempt to overcome this problem, a recombinant fusion protein was developed to serve as a sterilization vaccine in our laboratory (Fang et al, 2009(Fang et al, , 2010b.…”

Section: Introductionmentioning

confidence: 99%

“…However, such methods generally create some reaction products that require extensive purification in order to provide a homogenous protein (Ulker et al, 2005). The recombinant fusion protein consists of six copies of GnRH-I in a linear sequence (GnRH-I6) and maltosebinding protein (MBP) (MBP-GnRH-I6) (Fang et al, 2009(Fang et al, , 2010b. The recombinant fusion protein consists of six copies of GnRH-I in a linear sequence (GnRH-I6) and maltosebinding protein (MBP) (MBP-GnRH-I6) (Fang et al, 2009(Fang et al, , 2010b.…”

Section: Introductionmentioning

confidence: 99%

Immunization against recombinant GnRH-I alters testicular structure in an experimental boar model

Liu

et al. 2013

Zygote

Self Cite

View full text Add to dashboard Cite

The aim of this study was to evaluate and to compare testicular tissue in immunized and control boars. Eighteen male piglets, aged 12 weeks, were vaccinated twice intramuscularly with a maltose-binding protein-gonadotropin-releasing hormone I hexamer peptide (MBP-GnRH-I6). Blood samples were taken at 12, 18, 21 and 24 weeks of age. Serum concentrations of testosterone and GnRH-I antibodies were determined by radioimmunoassay. The pigs were sacrificed 6 weeks after the second immunization. Testicular weight and size were recorded and tissue samples were collected for histological examination. The results demonstrated that active immunization against MBP-GnRH-I6 increased serum GnRH-I antibody levels (P < 0.05) and reduced serum concentrations of testosterone (P < 0.05) when compared with controls. Histological studies performed on testicular tissue revealed clear signs of atrophy in the MBP-GnRH-I6 immunized pigs, and a significant reduction (P < 0.05) in paired testes weight and size were seen in the treated boars. Microscopically, the mean diameter of the seminiferous tubules was markedly reduced (P < 0.01). Spermatogonia were visible, as well as few spermatocytes, but no spermatozoa were detected in the seminiferous tubules. Ultramicroscopic analysis of testicular tissue revealed an increase in the thickness of the basement membrane and extensive damage in the cell organelles of the treated animals, including small spermatogonial size, decreased number of mitochondria and endoplasmic reticulum in the primary spermatocyte and spermatid, a shallow hollow for nuclear membranes in Sertoli cells and mitochondrial vacuolation in Leydig cells. We conclude that MBP-GnRH-I6 induces severe atrophy in the testes of immunized boars.

show abstract

“…The effectiveness of the recombinant protein in suppressing reproductive functions was demonstrated in boars (Fang et al . , b) and mice (Fang et al . ) and so on.…”

Section: Introductionmentioning

confidence: 96%

Effect of active immunization against GnRH‐I on the reproductive function in cat

Jiang

Hong

et al. 2015

Animal Science Journal

Self Cite

View full text Add to dashboard Cite

This study was designed to explore the effect of active immunization against maltose binding protein-gonadotropin releasing hormone I hexamer (MBP-GnRH-I6) on the reproductive function in cats. Each immunized cat was administered twice intramuscularly in the neck at 16 and 20 weeks old. The concentrations of the testosterone and estradiol and the level of anti-GnRH-I antibody in the serum were measured by radioimmunoassay and ELISA, respectively. The results showed that the weight and size of testicles and ovaries, and the concentrations of serum testosterone and estradiol in the immunized animals were lower than those of the control cats (P < 0.05), but that the levels of anti-GnRH-I antibody were significant higher compared to control animals (P < 0.05). Testicular tissues from the immunized male cats showed that seminiferous tubules were depauperate with the lumen relatively empty and that the differentiation of spermatogonia was not obvious. Tissues from the immunized female cats showed that the ovaries had many primordial follicles and primary follicles, but no secondary follicle was observed. These results showed active immunization against MBP-GnRH-I6 could make the gonads atrophy and reduce the concentrations of gonadal hormones, which suggested that MBP-GnRH-I6 was a very effective immunogen in the cat.

show abstract

Immunogenicity of Recombinant Maltose-binding Protein (MBP)–Gonadotropin Releasing Hormone I (GnRH-I)

Cited by 7 publications

References 29 publications

Immunization against recombinant GnRH-I alters ultrastructure of gonadotropin cell in an experimental boar model

Immunization against recombinant GnRH-I alters ultrastructure of gonadotropin cell in an experimental boar model

Immunization against recombinant GnRH-I alters testicular structure in an experimental boar model

Effect of active immunization against GnRH‐I on the reproductive function in cat

Contact Info

Product

Resources

About