Individual IL-3 priming is crucial for consistent in vitro activation of donor basophils in patients with chronic urticaria

Gentinetta, Thomas; Pecaric‐Petkovic, Tatjana; Wan, Daniel; Falcone, Franco H.; Dahinden, Clemens A.; Pichler, Werner J.; Hausmann, Oliver

doi:10.1016/j.jaci.2011.07.021

Cited by 49 publications

(59 citation statements)

References 49 publications

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“…Recently, the BAT has also been shown to correlate with the ASST as well as with the results of basophil histamine release assays (BHRA), and it has been proposed as a reliable functional assay in the diagnosis of autoimmune urticaria [16]. In contrast to the ASST, the BAT: (1) does not bear the risk of accidental infection by injections of autologous serum, (2) is not influenced by the intake of antihistamines, (3) provides quantifiable results, (4) is suited for the monitoring of treatment, (5) can distinguish between patients who express anti-IgE and anti-FcεRI antibodies, and (6) can be performed in patients of any age including infants [17,18,19]. …”

Section: Introductionmentioning

confidence: 99%

Positive CD63 Basophil Activation Tests Are Common in Children with Chronic Spontaneous Urticaria and Linked to High Disease Activity

Netchiporouk¹,

Moreau²,

Rahme³

et al. 2016

Int Arch Allergy Immunol

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Background: The basophil activation test (BAT) using CD63 expression is a sensitive and specific tool for the diagnostic workup of autoimmune chronic spontaneous urticaria (CSU). The definition of a positive BAT is directly dependent on the reference range and the cutoff values established in control populations. As of now, the pediatric reference range and cutoff values of the CD63 BAT remain to be established. Methods: In this study, we analyzed CD63 expression in 80 children (1-17 years old) without chronic urticaria (i.e., controls) and compared the values to those of a pediatric cohort of 105 CSU patients and 23 physical urticaria (PU) patients. Results: Based on the log-normal distribution of CD63 values in control subjects, the reference range and the cutoff for positive CD63 BAT values was established to be 1.2-1.8% (95% CI) and 1.8%, respectively. Children with CSU showed significantly elevated and significantly increased BAT values compared to healthy controls (Wilcoxon rank test p value <0.001). In contrast, no difference was found between BAT results in controls and PU patients. In pediatric CSU patients, a higher disease activity was associated with higher BAT values. Conclusions: Our study provides, for the first time, reference and cutoff values for the CD63 BAT in children. Our findings show that positive CD63 BAT are common in children with CSU and linked to a high disease activity.

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Section: Introductionmentioning

confidence: 99%

Positive CD63 Basophil Activation Tests Are Common in Children with Chronic Spontaneous Urticaria and Linked to High Disease Activity

Netchiporouk¹,

Moreau²,

Rahme³

et al. 2016

Int Arch Allergy Immunol

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“…The discrepancy between CD63 and CD203c correlations with ASST in our study can reflect either different mechanism of CD63 and CD203c molecules upregulation or differences in methodology of tests. Recently, Gentinetta et al [16] conducted the study of basophil activation tests in CU using different basophil donors and made a conclusion that CD63 activation test using IL-3 priming could be used as alternative to ASST. In authors opinion CD203c as basophil activation marker is less suitable for this purpose as they reported variable activation patterns, rendering the discrimination between patient and healthy control serum impossible in certain basophil donors.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, the ability of CU patient's serum to evoke expression of CD63 and CD203c on donor human basophils assessed by flow cytometry has been tested. There are a few published studies assessing serum-induced CD63 expression measurement as a diagnostic tool in CAU [11][12][13][14][15][16] and only 2 studies done by measured CD203c expression [16,17].…”

Section: Introductionmentioning

confidence: 99%

Serum induced CD63 and CD203c activation tests in chronic urticaria

Chomičienė¹,

Jurgauskienė²,

Kowalski

et al. 2014

Open Medicine

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“…The peripheral blood mononuclear cells were incubated with IL-3 (10 ng/ml; PeproTech EC Ltd., London, UK) for 30 min [11] and washed, then stimulated with dsDNA (1, or 10 μg/ml), rabbit anti-IgE Ab (0.1 μg/ml; DAKO Cytomation, Carpinteria, Calif., USA), or calcium ionophore A23187 (10 -6 M ) at 37°C for 30 min. A flow cytometric analysis of the mononuclear cells was performed as described previously [12].…”

Section: Methodsmentioning

confidence: 99%

Significantly High Levels of Anti-dsDNA Immunoglobulin E in Sera and the Ability of dsDNA to Induce the Degranulation of Basophils from Chronic Urticaria Patients

Hatada

Kashiwakura²,

Hayama³

et al. 2013

Int Arch Allergy Immunol

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Background: Chronic urticaria (CU) appears to be of autoimmune origin in about half of all patients, since several autoreactive immunoglobulin Gs (IgGs), such as anti-FcεRIα and anti-IgE, are detected in the sera of such patients. However, whether autoreactive IgE is associated with CU remains unclear. In this study, we attempted to identify autoreactive IgE antibodies in sera from patients with CU. Methods: Sera were collected from 67 normal subjects, 85 patients with CU and 28 patients with atopic dermatitis (AD). An autologous serum skin test (ASST) was performed on 27 of the CU patients. Autoreactive IgE and IgG levels against self-antigens were measured using enzyme-linked immunosorbent assays. The basophils were activated with dsDNA, and the CD63 expression level was examined using a fluorescence-activated cell sorter. Results: The anti-dsDNA IgE levels were significantly higher in patients with CU and AD than in normal subjects, but no differences in the anti-dsDNA IgG levels were seen. The levels of thioredoxin-, peroxiredoxin- and thyroglobulin-reactive IgE and IgG were not significantly higher in the CU patients than in the other 2 groups. There was no significant difference in the levels of anti-dsDNA IgE between ASST-positive and ASST-negative patients. The basophils from 2 out of 9 CU patients exhibited degranulation in response to dsDNA. Conclusions: Our data suggest that anti-dsDNA IgE is involved in the pathogenesis of some cases of CU.

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Individual IL-3 priming is crucial for consistent in vitro activation of donor basophils in patients with chronic urticaria

Cited by 49 publications

References 49 publications

Positive CD63 Basophil Activation Tests Are Common in Children with Chronic Spontaneous Urticaria and Linked to High Disease Activity

Positive CD63 Basophil Activation Tests Are Common in Children with Chronic Spontaneous Urticaria and Linked to High Disease Activity

Serum induced CD63 and CD203c activation tests in chronic urticaria

Significantly High Levels of Anti-dsDNA Immunoglobulin E in Sera and the Ability of dsDNA to Induce the Degranulation of Basophils from Chronic Urticaria Patients

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