Indirect spectrophotometric method for the determination of bisacodyl in commercial dosage forms and in environmental water samples

Majid, Nawal A.; Ahmad, Nief Rahman

doi:10.33899/iphr.2011.49908

Cited by 2 publications

(5 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Silica gel F 254 plates act as a stationary phase for both drugs. The mobile phase used was ethyl acetate:methanol:ammonium hydroxide (24:3:1, by volume) for LOP determination, while in the case of BIS, the mobile phase used was ethyl acetate:methanol:glacial acetic acid (85:10:5, by volume) …”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…On the other hand, bisacodyl (BIS, Figure b) is a synthetic pyridinylmethylene-diacetate ester derivative stimulant laxative indicated to treat constipation and bowel irregularity. , BIS is chemically [4-[(4-acetyloxyphenyl)-pyridin-2-ylmethyl]phenyl]acetate. Various analytical methods were described to determine BIS, including capillary electrophoresis, liquid and gas chromatography, − TLC-densitometric methods, spectrophotometry, , and electrochemical methods. , …”

Section: Introductionmentioning

confidence: 99%

“…22,23 BIS is chemically [4-[(4-acetyloxyphenyl)-pyridin-2-ylmethyl]phenyl]acetate. Various analytical methods were described to determine BIS, including capillary electrophoresis, 24 liquid and gas chromatography, 25−27 TLC-densitometric methods, 28 spectrophotometry, 29,30 and electrochemical methods. 31,32 Counterfeit medications containing falsified ingredients, where no active pharmaceutical constituent (APC) is added or the amount of the correct active constituent is substituted by other cheaper and incorrect amounts of APC such as acetaminophen (ACT), are considered one of the most serious risks to patient safety.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Smartphone as a Portable Detector for Thin-Layer Chromatographic Determination of Some Gastrointestinal Tract Drugs

et al. 2022

View full text Add to dashboard Cite

Thin-layer chromatography (TLC) is an effective and simple technique for screening, evaluating, and quantifying low-quality and counterfeit pharmaceutical products. Smartphones have recently been used as accessible, cheap, and portable detectors that can replace more complicated analytical detectors. In this work, we have developed a simple and sensitive TLC method utilizing a smartphone charged-coupled device (CCD) camera not only to verify and quantify some gastrointestinal tract drugs, namely, loperamide hydrochloride (LOP) and bisacodyl (BIS), but also to detect acetaminophen (ACT) as a counterfeit drug. Both drugs (LOP and BIS) were chromatographed separately on a silica gel 60 F 254 plate as a stationary phase under previously reported chromatographic conditions, using ethyl acetate:methanol:ammonium hydroxide (24:3:1, by volume) and ethyl acetate:methanol:glacial acetic acid (85:10:5, by volume) as developing systems to determine LOP and BIS, respectively. Universal stains, namely, iodine vapors and vanillin, were used to visualize the spots on the TLC plates to get a visual image using the smartphone camera and a spotlight as an illumination source with no need for a UV illumination source. The spot intensity was calculated using a commercially available smartphone application for quantitative analysis of the studied drugs utilizing ″acetaminophen″ as an example of a counterfeit substance. R f values were calculated using the recorded images and found to be 0.77, 0.79, and 0.74 for LOP, BIS, and ACT, respectively, providing drug identity. Linear calibration curves using the smartphone–TLC method were obtained between the luminance and the corresponding concentrations over the ranges of 2.00–10.00 μg/mL and 1.00–10.00 μg/mL with limits of detection of 0.57 and 0.10 μg/mL for LOP and BIS, respectively. The suggested method was validated according to the International Conference of Harmonization (ICH) guidelines. The method was then successfully applied for the qualitative and quantitative determination of LOP or BIS as an example for gastrointestinal tract drugs in pure form and in their pharmaceutical dosage formulations. The proposed method is considered as a perfect alternative to traditional reported densitometric methods due to its simplicity, easy application, and inexpensiveness. No previously reported methods utilizing smartphones have been published for the determination of the studied drugs. The developed approach is considered the first TLC method using smartphones for the determination of some gastrointestinal tract drugs in their pure form and in pharmaceutical formulations.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Smartphone as a Portable Detector for Thin-Layer Chromatographic Determination of Some Gastrointestinal Tract Drugs

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Various analytical methods were reported for quantitative analysis of BIS and its active metabolite including capillary electrophoresis [ 36 ], liquid and gas chromatography [ 37 – 39 ], thin layer chromatography [ 40 ], spectrophotometry [ 41 , 42 ] and electrochemical methods [ 43 , 44 ]. SEIRA could provide many advantages over the chromatographic methods due to portability, simplicity, short operation time, not requiring skilled operator, fewer trials, minimal waste generated, less sample size, and it is not affected by changes in temperature.…”

Section: Introductionmentioning

confidence: 99%

Comparing silver and gold nanoislands’ surface plasmon resonance for bisacodyl and its metabolite quantification in human plasma

Kelani,

Ibrahim,

Ramadan

et al. 2024

BMC Chemistry

View full text Add to dashboard Cite

Gold and silver nanoparticles have witnessed increased scientific interest due to their colourful colloidal solutions and exceptional applications. Comparing the localized surface plasmon resonance (LSPR) of gold and silver nanoparticles is crucial for understanding and optimizing their optical properties. This comparison informs the design of highly sensitive plasmonic sensors, aids in selecting the most suitable nanoparticles for applications like surface-enhanced infrared spectroscopy (SEIRA) and biomedical imaging, and guides the choice between gold and silver nanoparticles based on their catalytic and photothermal properties. Ultimately, the study of LSPR facilitates the tailored use of these nanoparticles in diverse scientific and technological applications. Two SEIRA methods combined with partial least squares regression (PLSR) chemometric tools were developed. This development is based on the synthesis of homogeneous, high-dense deposited metal nanoparticle islands over the surface of glass substrates to be used as lab-on-chip SEIRA sensors for the determination of bisacodyl (BIS) and its active metabolite in plasma. SEM micrographs revealed the formation of metallic islands of colloidal citrate-capped gold and silver nanoparticles of average sizes of 29.7 and 15 nm, respectively. BIS and its active metabolite were placed on the nanoparticles’ coated substrates to be directly measured, then PLSR chemometric modelling was used for the quantitative determinations. Plasmonic citrate-capped gold nanoparticle substrates showed better performance than those prepared using citrate-capped silver nanoparticles in terms of preparation time, enhancement factor, PLSR model prediction, and quantitative results. This study offers a way to determine BIS and its active metabolite in the concentration range 15–240 ng/mL in human plasma using inexpensive disposable glass-coated substrates that can be prepared in 1 h to get results in seconds with good recovery between 98.77 and 100.64%. The sensors provided fast, simple, selective, molecular-specific and inexpensive procedures to determine molecules in their pure form and biological fluid.

show abstract

Indirect spectrophotometric method for the determination of bisacodyl in commercial dosage forms and in environmental water samples

Cited by 2 publications

References 6 publications

Smartphone as a Portable Detector for Thin-Layer Chromatographic Determination of Some Gastrointestinal Tract Drugs

Smartphone as a Portable Detector for Thin-Layer Chromatographic Determination of Some Gastrointestinal Tract Drugs

Comparing silver and gold nanoislands’ surface plasmon resonance for bisacodyl and its metabolite quantification in human plasma

Contact Info

Product

Resources

About