Indirect selection of industrial tomato genotypes rich in zingiberene and resistant to Tuta absoluta Meyrick

Abstract

ABSTRACT. Obtaining tomato cultivars resistant to pests through interspecific crosses between commercial cultivars and wild accessions is an important tool in integrated pest management. The aim of this study was to select tomato genotypes with high zingiberene (ZGB) levels that are resistant to the South American tomato moth (Tuta absoluta Meyrick) and to estimate genetic parameters of ZGB inheritance from the interspecific cross Solanum lycopersicum cultivar 'Redenção' x Solanum habrochaites var. hirsutum (PI-127826 accession). F 2 plants with different ZGB contents were selected and submitted to a tomato moth resistance test. ZGB content exhibits high broad sense heritability, with incomplete dominance of lower ZGB content. Furthermore, the level of ZGB in leaflets was estimated to be controlled by two genes. These results show that high ZGB levels are effective at conferring resistance to the South American tomato moth.

Research Article Resistance to Helicoverpa armigera mediated by zingiberene and glandular trichomes in tomatoes for industrial processing

“…Resistance caused by zingiberene and type IV and type VI glandular trichomes, both via antixenosis and antibiosis has also been reported for other important pests of the tomato crop, such as tomato pinworm Tuta absoluta (Oliveira et al, 2012), the whitefly Bemisia tabaci (Silva et al, 2009), the two-spotted spider mite Tetranychus urticae (Gonçalves et al, 2006), among others. Lima et al (2015) found high estimates of heritability for the content of zingiberene in the leaves and found the level of this allelochemical is controlled by two genes with incomplete dominance in the sense of lower content, using populations from the crossing of the Redenção variety with accession 'PI-127826'. This may reflect satisfactory genetic gains over the backcrossing over generations, using the indirect selection for resistance to arthropods pest based on zingiberene contents.…”

“…In the population F 2 , (Lima et al 2015) selected plants with high contents of zingiberene in the leaves, which were used as a source of pollen for crossing with the Redenção variety and obtaining the generation F 1 from the first backcrossing. From self-fertilization of plants F 1 RC 1 , we obtained the segregating population (F 2 ) of the first backcrossing for the Redenção variety used in the present study.…”

“…The resistance is associated to the high concentration of the zingiberene allelochemical (ZGB) in the leaves, which is a compound derived from secondary metabolism, produced and exuded by type IV and type VI glandular trichomes (Carter et al, 1989;Freitas et al, 2002). Higher content of zingiberene and higher densities of these types of trichomes have been correlated with the increase in the resistance levels of tomatoes via the mechanisms of antixenosis and antibiosis (Gonçalves et al, 2006;Oliveira et al, 2012;Lima et al, 2015;Fernandes et al, 2016).…”

Tomato Breeding for Insect-Pest Resistance

“…Considering this, a rapid, low-cost spectrophotometric methodology was established for ZGB quantification in tomato leaves [81]. This method is now routinely applied in tomato breeding programs regarding pest insects and arachnids [22,26,82,83].…”

“…When investigating ZGB content inheritance in the interspecific intersection between S. lycopersicum and S. habrochaites var. hirsutum accession 'PI-127826', it was observed that leaf ZGB content is controlled by two genes with incomplete dominance in the sense of lower content, presenting greater heritability in the broad sense (81.9%) [26]. When evaluating the synergistic effect between high AA and ZGB leaf content, heterozygous genotypes were used for both characters [6], and the authors observed that the fact that heterozygous double genotypes show the same behavior as the heterozygotes only for ZGB or AA indicates that, although allelochemicals act similarly on the resistance of these genotypes to whitefly, they do not present a synergistic effect in this case.…”