Indirect plant regeneration in aromatic rice (Oryza sativa L.) var. ‘Kalijira’ and ‘Chinigura’ / POSREDNA REGENERACIJA AROMATIČNEGA RIŽA (Oryza sativa L.), SORT ‘KALIJIRA’ IN ‘CHINIGURA’

Abstract

Mature seeds of two traditional rice genotypes (Kalijira and Chinigura) were used for callus induction and plant regeneration on different concentrations and combinations of plant growth regulators cultured on MS (Murashige and Skoog) basal medium. Callus induction frequency was different between the cultivars, as well as among the 2,4-dichlorophenoxyacetic acid (2,4-D) levels tested. Both tested cultivars exhibited highest callus frequency at 2 mgl BAP and 1 mgl -1 IBA. Both rice genotypes are promising in terms of callus induction frequency and morphology, and regeneration ability of the embryogenic callus.

The Effects of Cytokinins on Plant Regeneration of Vetiver Grass (<i>Vetiveria nemoralis </i>A. Camus cv. Roiet)

“…However, in monocotyledon, callus induction and plantlet regeneration from various explants by using this technique are confronted with many problems such as the production of albino plant and the effect of phenolic compound [5]. Researchers have tried to enhance efficiency of plant regeneration and have achieved in many monocotyledon such as wheat [6], rice [7], maize [8] and sorghum [9]. Plant growth regulators are one of the most interested factor which are used in media for studying the development of plantlet.…”