Indirect enzyme-linked immunosorbent assay (Elisa) for detection of IgG antibodies against coxsackie B viruses

Coxsackie B virus-specific IgM antibody and myocardial infarction

“…The quality of the antigen preparation appears to have little bearing on the specificity of those tests in which the antigen is allowed to adsorb directly on to a solid phase. Even purified virus prepared by treatment with sodium lauryl sulphate to remove provirions (Brown et al, 1976) shows heterotypic activity when used under such conditions (Hannington et al, 1983). On the other hand, Katze and Crowell (1980) have shown that the type-specificity of purified coxsackie B viruses is preserved when they attach to a solid phase previously coated with specific 'capture' antibody.…”

“…Stocks were prepared in continuous African green monkey kidney (VERO) cells and stored at -70°C. Purified viruses for use as antigens in ELISA, and the control antigen, were prepared as described previously (Hannington et al, 1983).…”

“…The use of the sensitive, indirect enzyme-linked immunosorbent assay (ELISA) technique for detecting coxsackie B virus-specific IgG antibodies has not proved helpful for the diagnosis of recent infection, largely because it recognises group-en teroviral antibody, which masks the type-specific antibody response (Hannington et al, 1983). More recently, ELISA has been shown to be groupreactive for enterovirus IgM antibodies (Dorries and Ter Meulen, 1983;King et al, 1983;Pugh, 1984;Morgan-Capner and McSorley, 1983).…”