Indirect determination of mean whole body and intracellular CO2 and buffer capacity

Albers, C.; Ludwig, Otto; Usinger, W.; Spaich, P.

doi:10.1016/0034-5687(71)90024-7

Cited by 17 publications

(4 citation statements)

References 16 publications

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“…All the slopes depended on the nb buering capacities in the corresponding¯uid spaces. The functions of DpH ought to the best estimate of b; however, it has been found that DpH is not uniform throughout the dierent compartments in contrast to the almost constant D CO 2 (Albers et al 1971). The DpH in plasma of arterialized blood corresponds approximately to mean changes in extracellular¯uid, but the relationship between DpH plasma and DpH in the whole body is not known; in this case therefore only apparent buer capacities could be calculated.…”

Section: Carbon Dioxide Absorption and Bueringmentioning

confidence: 96%

“…the weighted averages of all compartments) should be determined. Experiments in dogs with ligated renal vessels using the DMO (5,5-dimethyl-2,4-oxazolidinedione) method for the measurement of mean intracellular pH have yielded 18 mmol á kgH 2 O A1 as whole body buer capacity (Albers et al 1971). A similar investigation in humans (with functioning kidneys) should be considered with caution because no intracellular buering at all has been observed during hypercapnia (Manfredi 1967).…”

Section: Introductionmentioning

confidence: 98%

“…The results of measurements have been very diverse (b = 14± 110 mmol á kg cell water A1 ) because of methodological problems and dierences between in vitro and in vivo estimations (Albers et al 1971;Bell and Wenger 1988;Heisler and Piiper 1971;Kemp et al 1993;Mizuno et al 1990;Sahlin 1978;Sharp et al 1986). Also little is known about the in¯uences of altitude.…”

Section: Introductionmentioning

confidence: 99%

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Carbon dioxide storage and nonbicarbonate buffering in the human body before and after an Himalayan expedition

Böning

Maassen

Steinacker

et al. 1999

European Journal of Applied Physiology

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Before and 7-12 days after an Himalayan expedition CO2 equilibration curves were determined in the blood plasma of 12 mountaineers by in vitro and in vivo CO2 titration; in vivo osmolality changes (delta Osm x deltaPCO2(-1), deltaOsm x delta pH(-1), where PCO2 is the partial pressure of CO2) during the latter experiments yielded estimates of whole body CO2 storage. In vitro -delta[HCO3-] x delta pH(-1) [nonbicarbonate buffer capacity (beta) of blood] was increased 7 days after descent [before 31.3 (SEM 0.4) mmol x kgH2O(-1), after 38.3 (SEM 3.9) mmol x kgH2O(-1); P<0.05] resulting from an increased proportion of young erythrocytes; in additional experiments an augmented beta was found in young (low density cells) compared to old cells [<1.097 g x ml(-1): 0.216 (SEM 0.028) mmol x gHb(-1), >1.100 g x ml(-1): 0.145 (SEM 0.013) mmol x gHb(-1), where Hb is haemoglobin; P < 0.02]. In spite of increased Hb mass in vivo delta[CO2total] x deltaPCO2(-1) [0.192 (SEM 0.010) mmol x kgH2O(-1) x mmHg(-1)] and -delta[HCO3-] x delta pH(-1) [17.9 (SEM 1.0) mmol x kgH2O(-1)] as indicators of extracellular beta rose only slightly after altitude (7 days +16%, P<0.02; +7%, NS) because of haemodilution. The deltaOsm x deltaPCO2(-1) [0.230 (SEM 0.015) mosmol x kgH2O(-1) x mmHg(-1)] remained unchanged. Prealtitude differences in deltaOsm x delta pH(-1) between hypercapnia [-41 (SEM 5) mosmol x kgH2O(-1)] and hypocapnia [-20 (SEM 3) mosmol x kgH2O(-1); P<0.01] disappeared temporarily after return since the former slope was reduced. The high value during hypercapnia before ascent probably resulted from mechanisms stabilizing intracellular pH during moderate hypercapnia which were attenuated after descent.

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Section: Carbon Dioxide Absorption and Bueringmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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Carbon dioxide storage and nonbicarbonate buffering in the human body before and after an Himalayan expedition

Böning

Maassen

Steinacker

et al. 1999

European Journal of Applied Physiology

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“…From the regression lines the buffer values were calculated according to the method of Albers et al (1). In 69 animals one renal artery was clamped to an outer diameter of slightly less than .2 mm 4 weeks prior to the experiment.…”

Section: Methodsmentioning

confidence: 99%

The intracellular pH and the buffer capacity of the heart

Albers

1977

Basic Res Cardiol

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The intracellular buffering (delta HCO3-/delta pH) in rats is lowest in skeletal muscle, intermediate in the diaphragm and highest in cardiac muscle. In the hypertrophic heart due to aortic constriction or to the Goldblatt mechanism the buffer value increases markedly during the first weeks, but returns to normal or even subnormal values during stable hypertrophy. There is an electrochemical disequilibrium for hydrogen ions caused by an active transport of ions across the cell membrane, which is possibly correlated to the myocardial activity.

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The intracellular pH and the buffer capacity of the heart

Albers¹

1977

The Hypertrophied Heart

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Indirect determination of mean whole body and intracellular CO2 and buffer capacity

Cited by 17 publications

References 16 publications

Carbon dioxide storage and nonbicarbonate buffering in the human body before and after an Himalayan expedition

Carbon dioxide storage and nonbicarbonate buffering in the human body before and after an Himalayan expedition

The intracellular pH and the buffer capacity of the heart

The intracellular pH and the buffer capacity of the heart

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