Indigo Naturalis Alleviates Dextran Sulfate Sodium-Induced Colitis in Rats via Altering Gut Microbiota

Sun, Zijie; Li, Junxiang; Dai, Yi; Wang, Wenting; Shi, Rui; Wang, Zhibin; Ding, Panghua; Lu, Qiongqiong; Jiang, Hui; Pei, Wenjing; Zhao, Xingjie; Guo, Yi; Jiali, Liu; Tan, Xiang; Mao, Tangyou

doi:10.3389/fmicb.2020.00731

Cited by 61 publications

(45 citation statements)

References 67 publications

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“…The concentrations of short-chain fatty acids (SCFAs) from each sample in the colon were determined by gas chromatograph (7890B-7000D, Agilent Technologies, Santa Clara, CA, USA) according to the method described in a previous study [ 19 ]. Briefly, 0.1 g of colonic digesta was mixed with 2 mL of 25% metaphosphoric acid solution and vortexed to homogenize.…”

Section: Methodsmentioning

confidence: 99%

Effects of Protein Restriction and Subsequent Realimentation on Body Composition, Gut Microbiota and Metabolite Profiles in Weaned Piglets

Hou

Wang

Qiu

et al. 2021

Animals

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The objective of this study was to evaluate the effects of protein restriction and subsequent protein realimentation on the body composition, gut microbiota and metabolite profiles of piglets. Fifty weaned piglets were randomly assigned to two treatments: a normal protein (NP) group (20% crude protein (CP)) or a low protein (LP) group (16% CP) with five animals per pen and five pens per group. Treatment diets were fed for 14 d during the protein restriction phase, and then all pigs were fed the same nursery diets with a normal CP level (19% CP) during the protein realimentation phase until they reached an average target body weight (BW) of 25 ± 0.15 kg. At day 14 and the end of the experiment, one piglet close to the average BW of each pen was slaughtered to determine body composition, microbial composition and microbial metabolites. Results showed that there was no difference (p > 0.05) in the experimental days to reach target BW between the LP and NP groups. The average daily gain (ADG) and gain:feed ratio (G:F) during the protein restriction phase as well as BW at day 14, were significantly decreased (p < 0.05) in the LP group compared with the NP group. However, there were no significant differences (p > 0.05) during the protein realimentation phase and the overall experiment. Similarly, piglets in the LP group showed a significantly decreased body protein content (p < 0.05) at day 14, but not (p > 0.05) at the end of the experiment. The relative abundance of Parabacteroides, Butyricicoccus, Olsenella, Succinivibrio and Pseudoramibacter were significantly increased (p < 0.05), while the relative abundance of Alloprevotella and Faecalicoccus were significantly decreased (p < 0.05) in the LP group at day 14. At the end of the experiment, the piglets in the LP group showed a higher (p < 0.05) colonic relative abundances of Parabacteroides, unidentified Christensenellaceae and Caproiciproducens, and a lower (p < 0.05) relative abundance of unidentified Prevotellaceae, Haemophilus, Marvinbryantia, Faecalibaculum, Neisseria and Dubosiella than those in the NP group. Metabolomics analyses indicated that tryptophan metabolism and vitamin metabolism were enriched in the LP group at day 14, and glycerophospholipid metabolism and fatty acid esters of hydroxy fatty acid metabolism were enriched at the end of the experiment. Moreover, Spearman’s correlation analysis demonstrated that the microbial composition was highly correlated with changes in colonic metabolites. Collectively, these results indicated that protein restriction and subsequent realimentation lead to compensatory growth and compensatory protein deposition in piglets and contribute to animal intestinal health by altering the gut microbiota and its metabolites.

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Section: Methodsmentioning

confidence: 99%

Effects of Protein Restriction and Subsequent Realimentation on Body Composition, Gut Microbiota and Metabolite Profiles in Weaned Piglets

Hou

Wang

Qiu

et al. 2021

Animals

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“…In IBD, the gut microbiota was associated with expansion of pro-in ammatory bacteria like Enterobacteriaceae and Fusobacteriaceae and reduction of anti-in ammatory species such as butyrate-producers within the Lachnospiraceae family [31,32]. In the current study, we identi ed four genera, namely Clostridium IV, Alistipes, Butyricicoccus, and Lachnospiraceae_incertae_sedis, to be positively associate with colitis alleviation in mice, all of which are known as SCFA-producing bacteria and have been broadly reported as bene cial microbes in colitis management [33,34]. As a genus in the family of Rikenellaceae, Alistipes, a relatively novel genus of bacteria, are highly relevant in dysbiosis and disease because Alistipes contain enzymes that can degrade cellulose, while loss of cellulose changed the microbial metabolome, gut immune response, gene expression in intestinal epithelial cells and the mice had an increased sensitivity to colitis induction [22,35], while the gut dysbiosis plays a role in determination of the compositional Alistipes abundance in the feces and associated with correlate with human health [22].…”

Section: Discussionmentioning

confidence: 64%

Gut microbiota-derived 5-hydroxyindole-3-acetic acid mediates the anti-colitis effect of Phellinus gilvus in mice

Li¹,

Sun²,

Su³

et al. 2020

Preprint

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Background: The gut microbiota plays crucial roles in various diseases and mediates the therapeutic effect of many drugs. Results: In this study, we found that ingestion of Phellinus gilvus extract (SH), a traditional Chinese medical mushroom, effectively ameliorated dextran sodium sulfate (DSS)-induced colits in mice, and the gut microbes were essential for its therapeutic effect. The enrichment of Alistipes spp. especially A. onderdonkii was highly associated with the amelioration of colitis by SH, and oral gavage of live A. onderdonkii potently and strain-specifically mitigated DSS-induced colitis in mice. 5-Hydroxyindole-3-acetic acid (5HIAA), a structural analogue of a known aromatic hydrocarbon receptor (AhR) activator indole-3-acetic acid (IAA), was significantly enriched after SH treatment and exhibited more potent anti-colitis efficiency than IAA in mice.Conclusion: Our results demonstrated the gut microbiota-derived 5HIAA could be an effective candidate in treatment of colitis, and modulation of the gut microbiota-indole derives-AhR pathway may be a novel strategy in development of next-generational anti-colitis drugs.

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“…The fecal transplantation experiment was performed based on our previous protocol ( Sun et al, 2020 ). Twenty donor mice were randomly divided into four groups including Control group, QCWZD group, DSS group and DSS + QCWZD group.…”

Section: Methodsmentioning

confidence: 99%

Qingchang Wenzhong Decoction Accelerates Intestinal Mucosal Healing Through Modulation of Dysregulated Gut Microbiome, Intestinal Barrier and Immune Responses in Mice

Sun

Wang³

et al. 2021

Front. Pharmacol.

Self Cite

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Inflammatory bowel disease (IBD), a group of multifactorial and inflammatory infirmities, is closely associated with dysregulation of gut microbiota and host metabolome, but effective treatments are currently limited. Qingchang Wenzhong Decoction (QCWZD) is an effective and classical traditional herbal prescription for the treatment of IBD and has been proved to attenuate intestinal inflammation in a model of acute colitis. However, the role of QCWZD in recovery phase of colitis is unclear. Here, we demonstrated that mice treated with QCWZD showed a faster recovery from dextran sulfate sodium (DSS)-induced epithelial injury, accompanied by reduced mucosal inflammation and attenuated intestinal dysbiosis using bacterial 16S rRNA amplicon sequencing compared to those receiving sterile water. The protective effects of QCWZD are gut microbiota dependent, as demonstrated by fecal microbiome transplantation and antibiotics treatment. Gut microbes transferred from QCWZD-treated mice displayed a similar role in mucosal protection and epithelial regeneration as QCWZD on colitis in mice, and depletion of the gut microbiota through antibiotics treatments diminished the beneficial effects of QCWZD on colitis mice. Moreover, metabolomic analysis revealed metabolic profiles alternations in response to the gut microbiota reprogrammed by QCWZD intervention, especially enhanced tryptophan metabolism, which may further accelerate intestinal stem cells-mediated epithelial regeneration to protect the integrity of intestinal mucosa through activation of Wnt/β-catenin signals. Collectively, our results suggested that orally administrated QCWZD accelerates intestinal mucosal healing through the modulation of dysregulated gut microbiota and metabolism, thus regulating intestinal stem cells-mediated epithelial proliferation, and hold promise for novel microbial-based therapies in the treatment of IBD.

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Indigo Naturalis Alleviates Dextran Sulfate Sodium-Induced Colitis in Rats via Altering Gut Microbiota

Cited by 61 publications

References 67 publications

Effects of Protein Restriction and Subsequent Realimentation on Body Composition, Gut Microbiota and Metabolite Profiles in Weaned Piglets

Effects of Protein Restriction and Subsequent Realimentation on Body Composition, Gut Microbiota and Metabolite Profiles in Weaned Piglets

Gut microbiota-derived 5-hydroxyindole-3-acetic acid mediates the anti-colitis effect of Phellinus gilvus in mice

Qingchang Wenzhong Decoction Accelerates Intestinal Mucosal Healing Through Modulation of Dysregulated Gut Microbiome, Intestinal Barrier and Immune Responses in Mice

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