Indigo biosynthesis by Comamonas sp. MQ

Qu, Yuanyuan; Zhang, Xuwang; Ma, Qiao; Ma, Fang; Zhang, Qiang; Li, Xinliang; Zhou, Hao; Zhou, Jiti

doi:10.1007/s10529-011-0778-2

Cited by 37 publications

(24 citation statements)

References 12 publications

(16 reference statements)

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“…Furthermore, the indigo formation by Pseudomonas sp. HAV-1 was superior when compared to other reported studies for indigo production ( Table 2 ) [ 21 , 22 ]. This implies that Pseudomonas sp.…”

Section: Resultsmentioning

confidence: 67%

Biotransformation of Indigo Pigment by Indigenously Isolated Pseudomonas sp. HAV-1 and Assessment of Its Antioxidant Property

Dua

Chauhan

Pathak

2014

Biotechnology Research International

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Chemical synthesis of indigo poses harsh environmental hazards and adverse human health effects. This necessitates an environment-friendly and producer-friendly approach for indigo production. The present study was thus significant as it reports an indigenously isolated potential indigo pigment producing culture identified as Pseudomonas sp. HAV-1 with noteworthy antioxidant property. The bioindigo pigment was characterized using various analytical techniques. The pigment production was enhanced from 412 μg mL−1 to 700 μg mL−1 by optimizing the growth parameters. Furthermore, the antioxidant property of indigo pigment is hitherto unexplored. This property can significantly append to its therapeutic potential. The bioindigo pigment produced by Pseudomonas sp. HAV-1 depicted 2.2 μM ascorbic acid equivalent antioxidant property. More to the point, the present work addresses a footstep towards green production of indigo.

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Section: Resultsmentioning

confidence: 67%

Biotransformation of Indigo Pigment by Indigenously Isolated Pseudomonas sp. HAV-1 and Assessment of Its Antioxidant Property

Dua

Chauhan

Pathak

2014

Biotechnology Research International

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“…Besides, product V with a retention time of 10.1 min and a prominent molecular ion (M-H) − peak at m/z 146.0246 (C 8 H 5 NO 2 ) was identified as isatin by comparing its mass spectral data with standard chemical and previous studies (Fig. 2e) [26,27]. Product VI was detected at retention time of 12.7 min with a prominent molecular ion (M-H) − peak at m/z 132.0454 (C 8 H 7 NO) (Fig.…”

Section: Mechanisms Of Indigoids Production By the Two Phenol-degradimentioning

confidence: 83%

“…In addition, indoxyl could be further hydroxylated to produce isatin [14,28]. Besides, isatin could also be obtained through the decomposition of indigo [26,28]. Previous studies indicated that the C-3 oxidation pathway of indole biotransformation usually occurred in accompany with C-2 and C-7 oxidation pathways, leading to the formation of 2-hydroxyindole (2-oxindole) and 7-hydroxyindole, respectively [14,29,30].…”

Section: Mechanisms Of Indigoids Production By the Two Phenol-degradimentioning

confidence: 99%

“…Previous studies indicated that the C-3 oxidation pathway of indole biotransformation usually occurred in accompany with C-2 and C-7 oxidation pathways, leading to the formation of 2-hydroxyindole (2-oxindole) and 7-hydroxyindole, respectively [14,29,30]. Condensation of indoxyl and 2-hydroxyindole/ isatin could yield indirubin [14,26,28,29], and 2-hydroxyindole was more preferable for indirubin production [17]. Furthermore, condensation of isatin and 7-hydroxyindole would lead to the formation of the purple product, 2-(7-oxo-1H-indol-6(7H)-ylidene) indolin-3-one [14].…”

Section: Mechanisms Of Indigoids Production By the Two Phenol-degradimentioning

confidence: 99%

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Indigoids Biosynthesis from Indole by Two Phenol-Degrading Strains, Pseudomonas sp. PI1 and Acinetobacter sp. PI2

Wang

Zhang

Fan

et al. 2015

Appl Biochem Biotechnol

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In this study, two phenol-degrading bacterial strains, designated as PI1 and PI2, were isolated from activated sludge for the production of indigoids from indole. According to the 16S ribosomal RNA (rRNA) gene sequence analysis, strains PI1 and PI2 were identified as Pseudomonas sp. and Acinetobacter sp., respectively. Liquid chromatography/time-of-flight/mass spectrometry (LC/TOF/MS) was applied to analyze the metabolites during the biotransformation of indole by the phenol-degrading strains. The results indicated that both strains could catalyze the formation of four indigoids with the same prominent molecular ion (M-H)(-) peak at m/z 261.067 and molecular formula of C16H10N2O2, including indigo and a purple product, 2-(7-oxo-1H-indol-6(7H)-ylidene) indolin-3-one. Isatin and 7-hydroxyindole were detected as the intermediates. Thus, the possible pathways for the production of indigoids from indole were proposed. Subsequently, the optimal conditions for the production of indigo from indole were determined using response surface methodology, and 11.82 ± 0.30 and 17.19 ± 0.49 mg/L indigo were produced by strains PI1 and PI2, respectively. The present study should provide potential candidates for microbial production of indigoids.

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“…While the productivity (135 mg/L) was still modest, this demonstrated that a fermentative approach can be effective. Since then, many other aromatic hydrocarbon-degrading bacteria have been identified and studied for their capability to produce indigo, often with indole as precursor (O'Connor and Hartmans 1998;Bhushan et al 2000;Alemayehu et al 2004;Pathak and Madamwar 2010;Qu et al 2012b). While many of these bacteria are pseudomonads, other bacteria have also been found to harbor NDOs that can be used to produce indigo (Mercadal et al 2010;Qu et al 2010).…”

Section: Naphthalene Dioxygenasesmentioning

confidence: 99%

An overview of microbial indigo-forming enzymes

Fabara

Fraaije

2019

Appl Microbiol Biotechnol

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Indigo is one of the oldest textile dyes and was originally prepared from plant material. Nowadays, indigo is chemically synthesized at a large scale to satisfy the demand for dyeing jeans. The current indigo production processes are based on fossil feedstocks; therefore, it is highly attractive to develop a more sustainable and environmentally friendly biotechnological process for the production of this popular dye. In the past decades, a number of natural and engineered enzymes have been identified that can be used for the synthesis of indigo. This mini-review provides an overview of the various microbial enzymes which are able to produce indigo and discusses the advantages and disadvantages of each biocatalytic system.

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Indigo biosynthesis by Comamonas sp. MQ

Cited by 37 publications

References 12 publications

Biotransformation of Indigo Pigment by Indigenously Isolated Pseudomonas sp. HAV-1 and Assessment of Its Antioxidant Property

Biotransformation of Indigo Pigment by Indigenously Isolated Pseudomonas sp. HAV-1 and Assessment of Its Antioxidant Property

Indigoids Biosynthesis from Indole by Two Phenol-Degrading Strains, Pseudomonas sp. PI1 and Acinetobacter sp. PI2

An overview of microbial indigo-forming enzymes

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