Indigenous Indonesian Wild Silkworm Cocoon of Attacus atlas as Biocompatible Film Biomaterial

Abstract

Abstract. The biocompatible film made from wild silkworm cocoon of Attacus atlas is intorduced in this research for anticipation of demand on biocompatible film for regenerative medicine. The wild silkworm cocoon was indigenous Indonesia and was taken from it original location in Indonesia. Protocol for degumming method was obtained in this research by using treatment with NaOH solution at 0.1 M for 1 hour. The film was prepared by grinding the wet degummed fiber until pulp like state was obtained. The mixture was dropped in sequence on the hot ceramic plate with temperature around 60 o C. The film thickness can be controlled precisely by using this technique. The film is soaked in alcohor for 1 day for stability testing and the result is found stable. The film is introduced in to COS-1 Cell suspension with previously washed in PBS solution and put in a chamber for biocompatibility testing. The cell are found able to grow and attach in first day observation and dramatically increase after 3 days observation. This is an indicate that the film that is produced from wild silkworm cocon of Attacus atlas has excellent biocompatibility.

The Effect of Vacuum Pressure during Autoclave Curing on Tensile Strength of Epoxy Composite Reinforced Natural Fiber of <i>Cordyline australis</i> with Sea Water Surface Treatment

“…Other natural product such as seaweed [12] are commonly use as medicine, coral reef [13][14][15] is used for fabrication of advance composite. Some importance natural fiber also studied as composite reinforcement such as silk [16][17][18][19][20][21][22], stinging nettle fiber [23], coconut coir [24], and fiber of Hibiscus tiliaceus [25].…”

Tensile Strength of Single Fiber of <i>Antheraea pernyi</i> Cocoon Prepared by Various Protocols

“…Other African source of non-mulberry wild silkworm cocoon also come into attention like Gonometapostica, Epiphorabauhiniae, Anaphe panda, and Argemamimosae [8]. In Japan, the wild silk from species Samia cynthiaricini was well studied and the fibroin from was isolated from its cocoons [9,10]. In Korea, the biomaterial film was developed by using fibroin from Antheraea pernyi [11] It is the purpose of this work to investigate the effect of various fiber yield protocols from species of Antheraea pernyi tensile strength and diameter of the fiber provided.…”

Comparison Tensile Strength of Natural and Synthetic Absorbable Sutures

“…Sutures can be made from nylon [2], polyethylene, polyester, copolymerized lactide and glycolide [1], Thermoplastic poly urethane (TPU) [3], polydioxanone, polyglycolic acid [4], polypropylene [3,4], silk [5][6][7][8], and catgut [9]. Two important properties are non-reactivity to provoke the least inflammatory response and failure load, to provide adequate tension for wound closure [10].…”