Indication of the impact of environmental stress on the responses of the bivalve mollusk Unio tumidus to ibuprofen and microplastics based on biomarkers of reductive stress and apoptosis

Мартынюк, В.; Khoma, Vira; Matskiv, Tetyana; Baranovsky, Vitaliy; Orlova-Hudim, Kateryna; Gylytė, Brigita; Symchak, Ruslan; Matciuk, Oksana; Gnatyshyna, Lesya; Manusadžianas, Levonas; Stoliar, Оksana

doi:10.1016/j.cbpc.2022.109425

Cited by 13 publications

(10 citation statements)

References 81 publications

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“…(Yang et al 2015). The same manifestation was observed in U. tumidus from two populations exposed to MP (1 mg L − 1 , size 0.1-0.5 mm), ibuprofen (0.8 µg L − 1 ) and their combination for 14 days(Martyniuk et al 2022b) and in Drosophila melanogaster after 10 h of starvation(Zhu et al 2012). However, this anaerobic shift during prolonged stress can be harmful to biological systems; for example, when the pearl oyster Pinctada margaritifera was exposed to 0.25, 2.5, and 25 µg L − 1 MP, a dose-dependent decrease in energy balance was observed under higher MP concentrations(Gardon et al 2020).…”

supporting

confidence: 66%

“…Two replicates from each group were provided. The obtained supernatant was subjected to the chromatography on Sephadex G-50 column in the same buffer as it was described in Martyniuk et al (2022b). Low weight (approximately 7 kDa) fractions with high absorbance at 254 nm and high D 254 /D 280 density ratio were identi ed as putative MTs-containing peak and pooled for the Zn determination.…”

Section: Assays Of Low Weight Thiols Zinc and Coppermentioning

confidence: 99%

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Reductive stress and apoptotic activities in bivalve mollusc Unio tumidus exposed to the combination of microplastics, antibiotic salinomycin and heating

Мартынюк

Matskiv

Yunko

et al. 2023

Preprint

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Multistress effects lead to unpredicted consequences in aquatic ecotoxicology and are extremely concerning. The goal of this study was to trace how specific effects of the antibiotic salynomycin (Sal) and microplastics (MP) on the bivalve molluscs are manifested in the combined environmentally relevant exposures. Unio tumidus specimens were treated with Sal (0.6 µg L− 1), MPs (1 mg L− 1, 2 µm size), and both at 18°C (Mix) and 25°C (MixT) for 14 days. The redox stress and apoptotic enzyme responses and the balance of Zn/Cu in the digestive gland were analyzed. The shared signs of stress included a decrease in NAD+/NADH and Zn/Cu ratios and lysosomal integrity, and an increase in Zn-metallothioneins and cholinesterase levels. MP caused a decrease in the glutathione (GSH) concentration and redox state, total antioxidant capacity, and Zn levels. MP and Mix induced coordinated apoptotic/autophagy activities, increasing caspase-3 and cathepsin D (CtD) total and extralysosomal levels. Sal activated caspase-3 only and increased by five times Cu level in tissue. Due to the discriminant analysis, the cumulative effect was evident in the combined exposure at 18°C. However, under heating, the levels of NAD+, NADH, GSH, GSH/GSSG and metallothionein-related thiols were decreased, and coordination of the cytosolic and lysosomal death stimuli was distorted, confirming that heating and pollution could exert unexpected synergistic effects on aquatic life.

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supporting

confidence: 66%

Section: Assays Of Low Weight Thiols Zinc and Coppermentioning

confidence: 99%

Reductive stress and apoptotic activities in bivalve mollusc Unio tumidus exposed to the combination of microplastics, antibiotic salinomycin and heating

Мартынюк

Matskiv

Yunko

et al. 2023

Preprint

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“…Bivalve molluscs like Mytilus galloprovincialis are valuable bioindicators due to their filter-feeding lifestyle, wide distribution, and ability to concentrate xenobiotics ( Pain-Devin et al, 2014 ; Freitas et al, 2020 ; Pagano et al, 2020 ; Curpan et al, 2022 ). Particularly, their validity to reflect the impact of micropollutants, like pharmaceuticals, has been proven in recent years ( Faggio et al, 2018 ; Piedade et al, 2020 ; Martyniuk et al, 2022a ; Martyniuk et al, 2022b ; Martyniuk et al, 2023 ; Chahouri et al, 2023 ; Impellitteri et al, 2023a ; Impellitteri et al, 2023b ). The pharmaceuticals represent a comparatively novel group of micropollutants of increasing concern given their widely discharged into water bodies on a continual basis ( Bottoni et al, 2010 ; Fabbri and Franzellitti, 2016 ; Turani et al, 2019 ; Martyniuk et al, 2023 ; Porretti et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Cellular and oxidative stress responses of Mytilus galloprovincialis to chlorpromazine: implications of an antipsychotic drug exposure study

Impellitteri,

Yunko,

Martyniuk

et al. 2023

Front. Physiol.

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Introduction: Bivalve molluscs like Mytilus galloprovincialis are valuable bioindicators due to their filter-feeding lifestyle, wide distribution, and ability to concentrate xenobiotics. Studying the effects of pharmaceuticals on these molluscs is crucial given their presence in surface waters. This study investigated the response of M. galloprovincialis to chlorpromazine (Cpz), an antipsychotic with antiviral activity against influenza, HIV, and coronaviruses in human cells.Methods: In this study, we examined the 14-day impact of chlorpromazine (Cpz) on the model species M. galloprovincialis at two concentrations (Cpz 1: 12 ng L-1 or 37 pM; Cpz 2: 12 µg L-1 or 37 nM). To ensure controlled exposure, a stock solution of Cpz was prepared and introduced into the tanks to match the intended concentrations. Seawater and stock solutions were refreshed every 48 h. The primary focus of this study centered on evaluating cell viability, cell volume regulation, and oxidative stress indicators.Results: Although cell volume regulation, as assessed by decreasing regulatory volume Regulation volume decrease, did not show statistically significant changes during the experiment, digestive cell viability, on the other hand, showed a significant decrease (p < 0.01) in the Cpz 2 group, suggesting effects on the general health and survival of these cells. Biochemically, in both Cpz 1 and Cpz 2, superoxide dismutase activity increased, while catalase (CAT) decreased, causing an elevated lipid peroxidation thiobarbituric acid-reactive substances and protein carbonyls, particularly in the Cpz 2 group. The level of reduced glutathione (GSH) increased in both exposures, whereas the level of GSSG increased only in the Cpz 1 group. Consequently, the GSH/GSSG ratio was elevated in the Cpz 2 group only.Discussion: A comparison of the magnitudes of anti- and pro-oxidative manifestations indicated a pro-oxidative shift in both exposures. These findings show that Cpz induces non-specific symptoms of biochemical and cellular disturbances in M. galloprovincialis even at the low picomolar concentration.

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“…Indeed, the state of the art on this substance showed levels of up to 20 μg L −1 of caffeine in raw sewage, rivers, drinking water, groundwater, and lakes, considering both the least and the most polluted river sites in Europe, with values ranging from 0.05 to 59.5 μg L −1 (Shulan et al, 2020; Vystavna, 2011; Wilkinson et al, 2022). Regarding MPs, on the other hand, the chosen concentration was largely based on previous research conducted in fresh waters (Martyniuk et al, 2023; Martyniuk, Gylytė, et al, 2022; Martyniuk, Khoma, et al, 2022); while the sizes correspond to those frequently observed in bivalves (1 mg L −1 of MPs with a particle size of 35–50 μm, corresponding to 15–70 MP elements L −1 . The powder was purchased from Sigma‐Aldrich, and corresponds to 434.272–ultra‐high molecular weight, surface‐modified, powdered polyethylene) (Sfriso et al, 2020).…”

Section: Methodsmentioning

confidence: 99%

“…Given that MPs are suspected to interact with hydrophobic substances, potentially acting as vectors and leading to increased contaminant accumulation, the potential influence of MPs on waterborne caffeine warrants investigation (Amelia et al, 2021; Burgos‐Aceves et al, 2022; de Sá et al, 2018; Menéndez‐Pedriza & Jaumot, 2020; Pastorino et al, 2023). Previous combined exposures of bivalve mollusks to MPs and various organic substances have demonstrated multistress effects distinct from single exposures (González‐Soto et al, 2019; Martyniuk et al, 2023; Martyniuk, Gylytė, et al, 2022; Martyniuk, Khoma, et al, 2022; Syberg et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

The odd couple: Caffeine and microplastics. Morphological and physiological changes in Mytilus galloprovincialis

Impellitteri,

Briglia,

Porcino

et al. 2024

Microscopy Res & Technique

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In recent years, the presence of pharmaceuticals and microplastics (MPs) in aquatic ecosystems has raised concerns about their environmental impact. This study explores the combined effects of caffeine, a common pharmaceutical pollutant, and MPs on the marine mussel Mytilus galloprovincialis. Caffeine, at concentrations of 20.0 μg L−1, and MPs (1 mg L−1, 35–50 μm size range), was used to mimic real‐world exposure scenarios. Two hundred M. galloprovincialis specimens were divided into four groups: caffeine, MPs, Mix (caffeine + MPs), and Control. After a two‐week acclimation period, the mollusks were subjected to these pollutants in oxygen‐aerated aquariums under controlled conditions for 14 days. Histopathological assessments were performed to evaluate gill morphology. Cellular volume regulation and digestive gland cell viability were also analyzed. Exposure to caffeine and MPs induced significant morphological changes in M. galloprovincialis gills, including cilia loss, ciliary disk damage, and cellular alterations. The chitinous rod supporting filaments also suffered damage, potentially due to MP interactions, leading to hemocyte infiltration and filament integrity compromise. Hemocytic aggregation suggested an inflammatory response to caffeine. In addition, viability assessments of digestive gland cells revealed potential damage to cell membranes and function, with impaired cell volume regulation, particularly in the Mix group, raising concerns about nutrient metabolism disruption and organ function compromise. These findings underscore the vulnerability of M. galloprovincialis to environmental pollutants and emphasize the need for monitoring and mitigation efforts.Research Highlights The synergy of caffeine and microplastics (MPs) in aquatic ecosystems warrants investigation. MPs and caffeine could affect gill morphology of Mytilus galloprovincialis. Caffeine‐exposed cells had lower viability than the control group in the NR retention test. MPs and mix‐exposed cells struggled to recover their volume.

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Indication of the impact of environmental stress on the responses of the bivalve mollusk Unio tumidus to ibuprofen and microplastics based on biomarkers of reductive stress and apoptosis

Cited by 13 publications

References 81 publications

Reductive stress and apoptotic activities in bivalve mollusc Unio tumidus exposed to the combination of microplastics, antibiotic salinomycin and heating

Reductive stress and apoptotic activities in bivalve mollusc Unio tumidus exposed to the combination of microplastics, antibiotic salinomycin and heating

Cellular and oxidative stress responses of Mytilus galloprovincialis to chlorpromazine: implications of an antipsychotic drug exposure study

The odd couple: Caffeine and microplastics. Morphological and physiological changes in Mytilus galloprovincialis

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