Indian Cassava Mosaic Virus: Ultrastructure of Infected Cells

Roberts, I. M.

doi:10.1099/0022-1317-70-10-2729

Cited by 19 publications

(5 citation statements)

References 25 publications

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“…Conversely, in virus‐infected pumpkin sink leaves, MPs of SqLCV (a phloem‐limited virus) and CaLCV (which infects all cell types) predominantly associate with ER‐derived tubules that penetrate the cell wall and are found only in undifferentiated vascular tissues (procambium) and in lesser amounts within the plasma membrane (Ward et al ., ). Furthermore, microtubules appear to play a role in MP–viral genome movement, as has been shown for Euphorbia mosaic virus (Kim and Lee, ) and Indian cassava mosaic virus (Roberts, ). In these cases, the MP‐containing tubules appear to serve as conduits for the intercellular transport of viral complexes.…”

Section: Movement Protein (Bc1 V2)mentioning

confidence: 71%

Geminivirus protein structure and function

Fondong

2013

Molecular Plant Pathology

234

137

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Geminiviruses are a family of plant viruses that cause economically important plant diseases worldwide. These viruses have circular single-stranded DNA genomes and four to eight genes that are expressed from both strands of the double-stranded DNA replicative intermediate. The transcription of these genes occurs under the control of two bidirectional promoters and one monodirectional promoter. The viral proteins function to facilitate virus replication, virus movement, the assembly of virus-specific nucleoprotein particles, vector transmission and to counteract plant host defence responses. Recent research findings have provided new insights into the structure and function of these proteins and have identified numerous host interacting partners. Most of the viral proteins have been shown to be multifunctional, participating in multiple events during the infection cycle and have, indeed, evolved coordinated interactions with host proteins to ensure a successful infection. Here, an up-to-date review of viral protein structure and function is presented, and some areas requiring further research are identified.

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Section: Movement Protein (Bc1 V2)mentioning

confidence: 71%

Geminivirus protein structure and function

Fondong

2013

Molecular Plant Pathology

234

137

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“…Woolston,J.I.I., 1990, personal communication) viruses, both leafhopper-transmitted, have random aggregates of particles like PSV-KB ( Fig. 2C) and show many similarities to BCTV virion aggregates (Esau & Hoefert, 1973) or Indian cassava mosaic virus (Roberts, 1989), both geminiviruses infecting dicotyledonous hosts, transmitted by leafhoppers and whitefiy, respectively. Tubular arrays of virions have been reported in plants infected by abutilon mosaic virus, a whitefiy-transmitted geminivirus (de Souza & Kim, 1990), and ribbon-like aggregates of particles in BCTV (Esau, 1977), but these were not found associated with the isolates in this study.…”

Section: Discussionmentioning

confidence: 99%

Viral inclusions in monocotyledons infected by maize streak and related geminiviruses

Pinner

Medina²,

Plaskitt

et al. 1993

Plant Pathology

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Isolates of maize streak virus (MSV) were examined by thin-section electron microscopy in plants, assessed for characteristic features of infection and compared with other related geminiviruses infecting monocotyledons from Africa, islands in the Indian Ocean, and the Pacific Island of Vanuatu. Arrays of virus particles, often crystalline, were most often seen in the nucleus. The morphology of the nuclear crystalline arrays was characteristic of certain isolates or groups of isolates (strains). Infected nuclei could be seen in cells from the phloem parenchyma, vascular bundle sheath and mesophyll tissue, and also in epidermal guard cells of plants infected with the maize strain of MSV. The particle arrays varied in morphology from regular rows of virions forming distinctive blocks, to randomly arranged aggregates in certain areas of the nucleus. We consistently failed to find viral crystalline arrays associated with infection of panicum streak virus (PSV) and sugar cane streak virus (SSV) isolates either in these hosts or in maize. Occasionally arrays of MSV particles were found outside the nuclear envelope in physiologically active cells. Accumulations or sheets of MSV particles were seen lining the walls ofsome phloem companion cells. Crystalline aggregates of particles were frequently observed in the cell vacuole. after lysis of the nuclear membrane of dead cells which made up the chlorotic lesions, the typical symptom of virus infection. Virus preparations from all hosts contained typical geminate particles regardless ofthe morphology ofthe virion arrays. The effect on chloroplasts appeared to vary between isolates and this is discussed in relation to lesion colour. The arrangement of virions in the nucleus as a taxonomic character is diagnostic for MSV. Inclusions with crystalline structure found in sieve elements of infected plants were not immunogold labelled when thin sections were probed using antiserum to the virus particles.

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“…On the basis of light and electron microscopic, cytological and immunological analyses it has been concluded that bipartite geminiviruses generally access non‐vascular tissues during the course of infection (e.g. Sequeira and Harrison, 1982; Rushing et al., 1987; Roberts, 1989; Wang et al., 1996). The process is mediated by DNA B genes BV1 and BC1 (Noueiry et al., 1994; Sanderfoot and Lazarowitz, 1995).…”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of Tissue Tropism of Bipartite Geminiviruses

Wege

Saunders

Stanley

et al. 2001

Journal of Phytopathology

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Abutilon mosaic virus (AbMV), a bipartite geminivirus of the genus Begomovirus, has been vegetatively propagated for many years in Abutilon sellovianum in which it is strictly phloem‐restricted. Using in situ hybridization and immunological analyses, the tissue tropism of AbMV in the laboratory host Nicotiana benthamiana was compared with that of two other bipartite begomoviruses, African cassava mosaic virus (ACMV) and tomato golden mosaic virus (TGMV). Analysis of the first systemically infected leaves and longitudinal sections of axillary and flower buds revealed that all three viruses are initially confined to the vascular traces, although both ACMV and TGMV are later detectable in nearly all tissue types. In contrast, AbMV remained strictly phloem‐limited in this host throughout the course of infection. The ability of ACMV and TGMV to move out of N. benthamiana phloem tissues is correlated with the development of severe symptoms in comparison with the mild symptoms associated with AbMV infection. It was also demonstrated that Sida micrantha mosaic virus, a virus that is closely related to AbMV, is phloem‐limited in Malva parviflora even though it induces severe leaf curl, stunting and necrosis in this host. The present data demonstrate that bipartite begomoviruses can exhibit strikingly different patterns of tissue tropism.

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Indian Cassava Mosaic Virus: Ultrastructure of Infected Cells

Cited by 19 publications

References 25 publications

Geminivirus protein structure and function

Geminivirus protein structure and function

Viral inclusions in monocotyledons infected by maize streak and related geminiviruses

Comparative Analysis of Tissue Tropism of Bipartite Geminiviruses

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