Comparative Analysis of Tissue Tropism of Bipartite Geminiviruses

Wege, Christina; Saunders, Keith; Stanley, John; Jeske, Holger

doi:10.1046/j.1439-0434.2001.00640.x

Cited by 56 publications

(27 citation statements)

References 66 publications

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“…Tissue sections of leaves, stems, axillary buds, and flowers were prepared as described previously (55,56).…”

Section: Methodsmentioning

confidence: 99%

“…Escape from the phloem to other leaf tissues has been reported for some dicotyledonous host-infecting begomoviruses, such as African cassava mosaic virus and Tomato golden mosaic virus in N. benthamiana (55), Bean dwarf mosaic virus in N. benthamiana and bean plants (52,53), Bean golden mosaic virus if coinfected with Tobacco mosaic virus (2), and mastreviruses which infect monocotyledonous hosts (25).…”

Section: Vol 78 2004 Tylcv and Tylcsv In Single Nuclei 10719mentioning

confidence: 98%

“…Tissue sections and isolated nuclei were hybridized as described previously (55). Biotinylated probes were detected with either streptavidin-alkaline phosphatase (AP) conjugate (catalogue no.…”

Section: Purification Of Nucleimentioning

confidence: 99%

“…Similar patterns of infection have been observed for the majority of geminiviruses that infect dicotyledonous hosts: AbMV in Abutilon spp., N. benthamiana, and Malva parviflora plants (17,55); Squash leaf curl virus in pumpkin plants (54); and Beet curly top virus in N. benthamiana (23). Escape from the phloem to other leaf tissues has been reported for some dicotyledonous host-infecting begomoviruses, such as African cassava mosaic virus and Tomato golden mosaic virus in N. benthamiana (55), Bean dwarf mosaic virus in N. benthamiana and bean plants (52,53), Bean golden mosaic virus if coinfected with Tobacco mosaic virus (2), and mastreviruses which infect monocotyledonous hosts (25).…”

Section: Vol 78 2004 Tylcv and Tylcsv In Single Nuclei 10719mentioning

confidence: 99%

See 3 more Smart Citations

Tête à Tête of Tomato Yellow Leaf Curl Virus and Tomato Yellow Leaf Curl Sardinia Virus in Single Nuclei

Morilla

Krenz

Jeske

et al. 2004

J Virol

105

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Since 1997 two distinct geminivirus species, Tomato yellow leaf curl Sardinia virus (TYLCSV) and Tomato yellow leaf curl virus (TYLCV), have caused a similar yellow leaf curl disease in tomato, coexisted in the fields of southern Spain, and very frequently doubly infected single plants. Tomatoes as well as experimental test plants (e.g., Nicotiana benthamiana) showed enhanced symptoms upon mixed infections under greenhouse conditions. Viral DNA accumulated to a similar extent in singly and doubly infected plants. In situ tissue hybridization showed TYLCSV and TYLCV DNAs to be confined to the phloem in both hosts, irrespective of whether they were inoculated individually or in combination. The number of infected nuclei in singly or doubly infected plants was determined by in situ hybridization of purified nuclei. The percentage of nuclei containing viral DNA (i.e., 1.4% in tomato or 6% in N. benthamiana) was the same in plants infected with either TYLCSV, TYLCV, or both. In situ hybridization of doubly infected plants, with probes that discriminate between both DNAs, revealed that at least one-fifth of infected nuclei harbored DNAs from both virus species. Such a high number of coinfected nuclei may explain why recombination between different geminivirus DNAs occurs frequently. The impact of these findings for epidemiology and for resistance breeding concerning tomato yellow leaf curl diseases is discussed.

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“…Tissue sections of leaves, stems, axillary buds, and flowers were prepared as described previously (55,56).…”

Section: Methodsmentioning

confidence: 99%

Section: Vol 78 2004 Tylcv and Tylcsv In Single Nuclei 10719mentioning

confidence: 98%

Section: Purification Of Nucleimentioning

confidence: 99%

Section: Vol 78 2004 Tylcv and Tylcsv In Single Nuclei 10719mentioning

confidence: 99%

See 2 more Smart Citations

Tête à Tête of Tomato Yellow Leaf Curl Virus and Tomato Yellow Leaf Curl Sardinia Virus in Single Nuclei

Morilla

Krenz

Jeske

et al. 2004

J Virol

105

View full text Add to dashboard Cite

show abstract

“…Most geminiviruses are confined to the phloem, whereas some can emigrate to mesophyll cells in natural or model hosts (reviewed in reference 9). The Abutilon mosaic virus (AbMV), used in this study, is strictly phloem limited (10)(11)(12) and infects nuclei of companion and phloem parenchyma cells (13,14).…”

mentioning

confidence: 99%

A Population Genetics Perspective on Geminivirus Infection

Weigel

Pohl

Wege

et al. 2015

J Virol

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The selective accumulation of both DNA components of a bipartite geminivirus, Abutilon mosaic virus, was recorded during early systemic infection of Nicotiana benthamiana plants. Purified nuclei were diagnosed for viral DNA using hybridization specific for DNA A or DNA B to detect these individual genome components either alone or both simultaneously by dual-color staining. Although this virus needs both components for symptomatic infection, DNA A alone was transported to upper leaves, where it was imported into phloem nuclei and replicated autonomously. The coinfection with DNA A and DNA B revealed an independent spread of both molecules, which resulted in a stochastic distribution of DNA A-and DNA A/B-infected nuclei. A population genetics evaluation of the respective frequencies was compared to a model computation. This elucidated a surprisingly simple relationship between the initial frequencies of the viral DNA components and the number of susceptible cells during the course of early systemic infection. IMPORTANCEFor bipartite begomoviruses, DNA B-independent long-distance spread of DNA A has been described before, but it has never been shown whether viral DNA A alone invades nuclei of systemic tissues and replicates therein. This is demonstrated now for the first time. During infection with DNA A and DNA B, a similar solitary spread of DNA A can be recognized at early stages. We describe a population genetics model of how the hit probabilities of DNA A and DNA B for susceptible cells determine the relative frequencies of either genome component during the course of infection.

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Movement Proteins (BC1 and BV1) of Abutilon Mosaic Geminivirus Are Cotransported in and between Cells of Sink but Not of Source Leaves as Detected by Green Fluorescent Protein Tagging

2001

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Two movement proteins (BV1 and BC1) facilitate the intra- and intercellular transport of begomoviruses in plants. In contrast to other geminiviruses the movement protein BC1 of Abutilon mosaic virus (AbMV) remained in the supernatant after centrifuging plant extracts at 20,000 g. To test whether this unusual behavior results from a distinct intracellular distribution of the protein, the BC1 gene has been fused to the gene of green fluorescent protein (GFP). The resulting plasmids were delivered into nonhost plants (Allium cepa) as well as into mature and immature cells of host plants (Nicotiana tabacum, N. benthamiana) by biolistic bombardment for transient expression in planta. BC1 directed GFP to two different cellular sites. In the majority of nonhost cells as well as in mature cells of host leaves, BC1 was mainly localized in small punctate flecks at the cell periphery or, to a lesser extent, around the nucleus. In sink leaves of host plants, GFP:BC1 additionally developed disc-like structures in the cell periphery. Cobombardment of GFP:BC1 with its cognate infectious DNA A and B did not change their subcellular distribution patterns in source leaves but led to the formation of peculiar needle-like structures in sink leaves. The nuclear shuttle protein (BV1) of AbMV accumulated mainly inside the nuclei as shown by immunohistochemical staining and GFP tagging. In sink cells of host plants it was mobilized to the plasma membrane and to the nucleus of the neighboring cell by coexpressed BC1, GFP:BC1, BC1:GFP, or after cobombardment with the cognate viral DNA. Only under these conditions were GFP:BC1 and BC1:GFP also found in the recipient cell.

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Comparative Analysis of Tissue Tropism of Bipartite Geminiviruses

Cited by 56 publications

References 66 publications

Tête à Tête of Tomato Yellow Leaf Curl Virus and Tomato Yellow Leaf Curl Sardinia Virus in Single Nuclei

Tête à Tête of Tomato Yellow Leaf Curl Virus and Tomato Yellow Leaf Curl Sardinia Virus in Single Nuclei

A Population Genetics Perspective on Geminivirus Infection

Movement Proteins (BC1 and BV1) of Abutilon Mosaic Geminivirus Are Cotransported in and between Cells of Sink but Not of Source Leaves as Detected by Green Fluorescent Protein Tagging

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