Indexing Tools for Indian Citrus Ringspot Virus (ICRSV)

Technical advancement in plant virus diagnosis – an appraisal

“…and Chenopodiaceae and Solanaceae for nepoviruses such as SLRV, CLRV, etc. ), use of herbaceous plants for indexing is limited compared to woody indicators, as less than one-third of the known fruit tree viruses may get transmitted mechanically (Gilles and Bormans 1988;Sharma et al 2009). In spite of the fact that woody indexing is labour intensive, tedious and time consuming, expensive, requires weeks to months to obtain results, requires adequate facilities such as greenhouse for biological tests, fields for bioassay, store house, screen house and a diagnostic laboratory, it is compulsory in any certification programme as all diseases irrespective of causal agents are detected by grafting.…”

“…Initially, viral disease could be diagnosed quickly by visual examination of symptoms that they cause in different host species, by studying their vector transmission properties along with the study of their interaction with the hosts. Variants that have broad host range but are often symptomless or produce symptoms showing resemblance with other viruses require molecular tests for diagnosis as these techniques are more sensitive and reproducible and allow the typification of more isolates than bioassays (Garcia-Arenal et al 2001;Sharma et al 2009). For the development of efficient and stable diagnostic methods, the following factors should be considered: (1) sensitivity, i.e.…”