Independent regulation of cellular properties in thermosensitive transformation mutants of mouse fibroblasts

Ps, Rudland; Pearlstein, Edward; Kamely, Daphne; Nutt, Max; Eckhart, Walter

doi:10.1038/256043a0

Cited by 7 publications

(9 citation statements)

References 22 publications

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“…The difference in serum-dependent caseinolytic activity (probably plasminogen activator) was not so large as for simian virus-transformed and nontransformed mouse 3T3 fibroblasts (Table I) (28).…”

Section: Cell Propertiesmentioning

confidence: 78%

“…GROWTH IN AGAROSE: The slow-sticking epithelial fractions as described above were plated at 10"~/ 3.5-cm dish in quadruplicate in DEM and 10% FCS. Cells were overlayed with a suspension of 0.33% agarose and 10% FCS in DEM for 2 wk at 37~ (28). By this time, any clumps of normal cells which were obtained immediately after plating had disintegrated.…”

Section: Casein Synthesismentioning

confidence: 99%

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Mitogenic activity of pituitary hormones on cell cultures of normal and carcinogen-induced tumor epithelium from rat mammary glands

Ps¹,

Hallowes²,

Durbin³

et al. 1977

The Journal of Cell Biology

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Cell suspensions containing normal or tumor epithelium were readily obtained by enzymatically digesting rat mammary glands from perphenazine-treated (prolactin-hypersecreting) cycling, female virgin animals or hormone-responsive mammary tumors from animals treated with dimethylbenzanthracene. Cell suspensions were fractionated into predominantly epithelial and predominantly stromal cells by their differential rates of attachment to culture dishes. Both normal mammary and tumor epithelial cells were characterized by the presence of specific celljunctional complexes, desmosome-like structures, surface microvilli, and their ability to synthesize casein. Serum-dependent protease activity was greater in cultures derived from tumors, and cells from such cultures grew in agarose whereas those from the non-neoplastic gland did not. The addition of prolactin to the culture medium stimulated DNA synthesis in primary or secondary epithelial cultures from tumors, whereas additional insulin and hydrocortisone with prolactin were required for similar levels of DNA synthesis in cultures from nonneoplastic glands. The fraction of cells synthesizing DNA was, however, smaller than that with 10% serum measured in the same time period. Both growth hormone and epidermal growth factor stimulated DNA synthesis but to a lesser extent than did prolactin. Prolactin with hydrocortisone and insulin was relatively inactive in promoting DNA synthesis of the nonepithelial cells whereas pituitary fibroblast growth factor was more active. These mitogenic effects were obtained when the hormones were added to the medium at near physiological concentrations, and paralleled the known activities of the hormones in control of mammary gland growth and development in the rat.Growth and development of the mammary gland in mice and rats occur mainly by a process of cell multiplication rather than by hypertrophy of existing cellular units (9). The hormones which control this process in vivo have largely been identified by means of a series of endocrine gland-ablation and hormone-replacement experiments, and they include prolactin, growth hormone together with estrogens, glucocorticoids, and progesterone (18,21,34,35). In organ culture, however, Topper and others have failed to show any mitogenic effect of prolactin or growth hormone although high, nonphysiological concentrations of insulin and a corticoid were shown to stimulate thymidine incorporation into DNA (19,22,33,38,40). Since pretreatment of virgin mice with prolactin

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Section: Cell Propertiesmentioning

confidence: 78%

Section: Casein Synthesismentioning

confidence: 99%

Mitogenic activity of pituitary hormones on cell cultures of normal and carcinogen-induced tumor epithelium from rat mammary glands

Ps¹,

Hallowes²,

Durbin³

et al. 1977

The Journal of Cell Biology

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“…Two cell systems which can be reproducibly transformed in vitro with chemical carcinogens have been studied; Syrian hamster embryo cell cultures and guinea-pig fetal cell cultures. Plasminogen activator synthesis is of interest because of the evidence that cells transformed in vitro by viruses or chemical carcinogens frequently synthesize much more of this protease than their normal counterparts (Christman et al, 1975;Goldberg, 1974;Laug et al, 1975;Ossowski er al., 1973;Pollack and Rifkin, 1975;Pollack et al, 1975;Rudland et al, 1975;Unkeless et al, 1973). Exceptions to this association have been observed (Gunn et al, 1977;Hynes and Pearlstein, 1976;Jones et al, 1976;Montesano et al, 1977;Mott et al, 1974;Pearlstein et al, 1976;Rifkin and Pollack, 1977;Rohrlich and Rifkin, 1977;Wilson and Reich, 1979).…”

mentioning

confidence: 94%

Plasminogen activator synthesis accompanying chemical carcinogen‐induced in vitro transformation of syrian hamster and guinea‐pig fetal cells

Slsskin

Weinstein

Evans

et al. 1980

Intl Journal of Cancer

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The production of the extracellular protease, plasminogen activator, in relationship to growth in semi-solid medium and tumorigenicity has been studied in Syrian hamster embryo and strain 2 guinea-pig embryo cell culture models of chemical carcinogenesis. Whereas normal hamster and guinea-pig embryo cells had negligible levels of plasminogen activator, neoplastically transformed cells derived from cultures exposed to chemical carcinogens had high levels of plasminogen activator and grew as progressively enlarging colonies in soft agar. The development of plasminogen activator secretion in relation to neoplastic transformation was further studied in the guinea-pig cells where the latent period between carcinogen exposure and neoplastic transformation ranged from 4 months to more than one year. The production of plasminogen activator in this system also exhibited a long latent period, and acquisition of extracellular plasminogen activator correlated temporally with growth in agar and tumorigenicity. Plasminogen activator and growth in semi-solid agar in concert are useful markers for identification of neoplastic cells transformed in culture following exposure to a chemical carcinogen.

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“…Evidence exists for an association between high levels of activator and the permissive temperature in virustransformed temperature-sensitive mutants (Unkeless et al, 1973;Rudland et al, 1975). Furthermore a number of features of transformation have been shown to be dependent upon or inducible by plasminogen activator.…”

mentioning

confidence: 99%

“…Furthermore a number of features of transformation have been shown to be dependent upon or inducible by plasminogen activator. These include transformed morphology (Ossowski et at., 1973a(Ossowski et at., , 1973bWeber, 1975;Urquhart et al, 1978), agglutination with plant lectins (Whur et al, 1976), growth in soft agar and loss of anchorage dependence (Ossowski et al, 1973b;Pollack et al, 1974;Laug et al, 1975;Rudland et al, 1975;Kamely, 1976), metastasis (Peterson et al, 1973), migration and invasiveness (Ossowski et al, 1973b), altered cell adhesion (Weber, 1975) and the onset of DNA synthesis in previously quiescent confluent monolayers (Urquhart et al, 1978). There are, however, reports of an absence of correlation between plasminogen activation and malignancy or transformation (Hisazumi & Fukushima, 1973;Mott et al, 1974;Chibber et al, 1975), transformed morphology (Chen & Buchanan, 1.975;Barrett et al, 1977), increased hexose uptake (Chen & Buchanan, 1975;Weber, 1975), growth in soft agar (Jones et al, 1975c) and growth inhibition in transformed cells (Chou et al, 1974;Roblin et al, 1975).…”

mentioning

confidence: 99%

Plasminogen activation transforms the morphology of quiescent 3T3 cell monolayers and initiates growth

Whur¹,

Silcox²,

Boston³

et al. 1979

Br J Cancer

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Independent regulation of cellular properties in thermosensitive transformation mutants of mouse fibroblasts

Cited by 7 publications

References 22 publications

Mitogenic activity of pituitary hormones on cell cultures of normal and carcinogen-induced tumor epithelium from rat mammary glands

Mitogenic activity of pituitary hormones on cell cultures of normal and carcinogen-induced tumor epithelium from rat mammary glands

Plasminogen activator synthesis accompanying chemical carcinogen‐induced in vitro transformation of syrian hamster and guinea‐pig fetal cells

Plasminogen activation transforms the morphology of quiescent 3T3 cell monolayers and initiates growth

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