Independent activation of the BoMYB2 gene leading to purple traits in Brassica oleracea

Yan, Chenghuan; An, Guanghui; Zhu, Ting; Zhang, Weiyi; Zhang, Lei; Peng, Liying; Chen, Jiongjiong

doi:10.1007/s00122-018-3245-9

Cited by 62 publications

(55 citation statements)

References 53 publications

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“…Anthocyanin biosynthesis and regulation pathway has been well studied in Cruciferae plants including Arabidopsis, Brassica rapa, Brassica oleracea, Brassica napus, etc. (Guo et al, 2014;Xie Y. et al, 2016;Goswami et al, 2018;Yan et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Genetic and Comparative Transcriptome Analysis Revealed DEGs Involved in the Purple Leaf Formation in Brassica juncea

et al. 2020

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Brassica juncea is an important dietary vegetable cultivated and consumed in China for its edible stalks and leaves. The purple leaf mustard, which is rich in anthocyanins, is eye-catching and delivers valuable nutrition. However, the molecular mechanism involved in anthocyanin biosynthesis has not been well studied in B. juncea. Here, histological and transcriptome analyses were used to characterize the purple leaf color and gene expression profiles. Free-hand section analysis showed that the anthocyanin was mainly accumulated in the adaxial epidermal leaf cells. The anthocyanin content in the purple leaves was significantly higher than that in the green leaves. To investigate the critical genes and pathways involved in anthocyanin biosynthesis and accumulation, the transcriptome analysis was used to identify the differentially expressed genes (DEGs) between the purple and green leaves from the backcrossed BC3 segregation population in B. juncea. A total of 2,286 different expressed genes were identified between the purple and green leaves. Among them, 1,593 DEGs were up-regulated and 693 DEGs were down-regulated. There were 213 differently expressed transcription factors among them. The MYB and bHLH transcription factors, which may regulate anthocyanin biosynthesis, were up-regulated in the purple leaves. Interestingly, most of the genes involved in plant-pathogen interaction pathway were also up-regulated in the purple leaves. The late biosynthetic genes involved in anthocyanin biosynthesis were highly upregulated in the purple leaves of B. juncea. The up regulation of BjTT8 and BjMYC2 and anthocyanin biosynthetic genes (BjC4H, BjDFR, and BjANS) may activate the purple leaf formation in B. juncea. This study may help to understand the transcriptional regulation of anthocyanin biosynthesis in B. juncea.

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Section: Introductionmentioning

confidence: 99%

Genetic and Comparative Transcriptome Analysis Revealed DEGs Involved in the Purple Leaf Formation in Brassica juncea

et al. 2020

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“…Similarly, only 213 DEGs between two parents were identified via transcriptome sequencing. This number is far less than that gained in several recent transcriptome studies on Brassica oleracea species [19,20], but similar to the 306 DEGs uncovered between a pair of near-isogenic lines of cotton [21]. Such a limited number of candidate SNPs and DEGs were attributed to the highly uniform genetic background between two parents, which could have filtered out a lot of false positives, and therefore significantly enhanced the efficiency of BSA-seq and RNA-seq analysis.…”

Section: Discussionmentioning

confidence: 80%

Identification of Candidate Genes Involved in Curd Riceyness in Cauliflower

Zhao

Sheng

et al. 2020

IJMS

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“Riceyness” refers to the precocious development of flower bud initials over the curd surface of cauliflower, and it is regarded as undesirable for the market. The present study aimed to identify the candidate loci and genes responsible for the morphological difference in riceyness between a pair of cauliflower sister lines. Genetic analysis revealed that riceyness is controlled by a single dominant locus. An F2 population derived from the cross between these sister lines was used to construct “riceyness” and “non-riceyness” bulks, and then it was subjected to BSA-seq. On the basis of the results of Δ(SNP-index) analysis, a 4.0 Mb candidate region including 22 putative SNPs was mapped on chromosome C04. Combining the RNA-seq, gene function annotation, and target sequence analysis among two parents and other breeding lines, an orthologous gene of the Arabidopsis gene SOC1, Bo4g024850 was presumed as the candidate gene, and an upstream SNP likely resulted in riceyness phenotype via influencing the expression levels of Bo4g024850. These results are helpful to understand the genetic mechanism regulating riceyness, and to facilitate the molecular improvement on cauliflower curds.

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“…There are multiple examples where recruitment of a TE in the promoter region of MYB10 orthologs leads to up-regulation of MYB expression and anthocyanin accumulation in other species such as orange, apple, pepper or brassica (Butelli et al, 2012;Jung et al, 2019;Yan et al, 2019;Zhang et al, 2019). Remarkably, in B. oleracea and pepper, the TE identified to enhance the expression of the host MYB gene is, as FaEnSpm-2, a CACTA element (Yan et al, 2019;Jung et al, 2019). To investigate the association of FaEnSpm-2 with red flesh color, we profiled global gene expression using RNA-seq in fruits from 'Senga Sengana' and the six white-fleshed accessions previously genotyped in this study except for USA2.…”

Section: Expression Of Myb10 and Anthocyanin Biosynthetic Genes Are Umentioning

confidence: 99%

Allelic Variation ofMYB10is the Major Force Controlling Natural Variation of Skin and Flesh Color in Strawberry (Fragariaspp.) fruit

Castillejo

Waurich

Ramos

et al. 2020

Preprint

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Anthocyanins are the principal color-producing compounds synthesized in developing fruits of strawberry (Fragaria spp.). Substantial natural variation in color have been observed in fruits of diploid and octoploid accessions, resulting from distinct accumulation and distribution of anthocyanins in fruits. Anthocyanin biosynthesis is controlled by a clade of R2R3 MYB transcription factors, among which MYB10 has been shown as the main activator in strawberry fruit. Here, we show that MYB10 mutations cause most of the anthocyanin variation observed in diploid woodland strawberry (F. vesca) and octoploid cultivated strawberry (F. ×ananassa). Using a mapping-by-sequencing approach, we identified a gypsytransposon insertion in MYB10 that truncates the protein and knocks out anthocyanin biosynthesis in a white-fruited F. vesca ecotype. Two additional lossof-function MYB10 mutations were identified among geographically diverse whitefruited F. vesca ecotypes. Genetic and transcriptomic analyses in octoploid Fragaria spp. revealed that FaMYB10-2, one of three MYB10 homoeologs identified, residing in the F. iinumae-derived subgenome, regulates the biosynthesis of anthocyanins in developing fruit. Furthermore, independent mutations in MYB10-2 are the underlying cause of natural variation in fruit skin and flesh color in octoploid strawberry. We identified a CACTA-like transposon (FaEnSpm-2) insertion in the MYB10-2 promoter of red-fleshed accessions that was associated with enhanced expression and anthocyanin accumulation. Our findings suggest that putative cis regulatory elements provided by FaEnSpm-2 are required for high and ectopic MYB10-2 expression and induction of anthocyanin biosynthesis in fruit flesh. We developed MYB10-2 (sub-genome) specific DNA markers for marker-assisted selection that accurately predicted anthocyanin phenotypes in octoploid segregating populations.

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Independent activation of the BoMYB2 gene leading to purple traits in Brassica oleracea

Cited by 62 publications

References 53 publications

Genetic and Comparative Transcriptome Analysis Revealed DEGs Involved in the Purple Leaf Formation in Brassica juncea

Genetic and Comparative Transcriptome Analysis Revealed DEGs Involved in the Purple Leaf Formation in Brassica juncea

Identification of Candidate Genes Involved in Curd Riceyness in Cauliflower

Allelic Variation ofMYB10is the Major Force Controlling Natural Variation of Skin and Flesh Color in Strawberry (Fragariaspp.) fruit

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