Incubation time after pulsed electric field treatment of microalgae enhances the efficiency of extraction processes and enables the reduction of specific treatment energy

Silve, Aude; Kian, Chua Boon; Papachristou, Ioannis; Kubisch, Christin; Nazarova, N. I.; Wüstner, Rüdiger; Leber, K.; Sträßner, R.; Frey, Wolfgang

doi:10.1016/j.biortech.2018.08.060

Cited by 48 publications

(56 citation statements)

References 41 publications

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“…Nevertheless, new studies also show that the mass transfer is further enhanced by simply incubating the permeabilized cells after the PEF treatment. The associated decrease of the transport resistance is believed to be caused by an enzymatically driven autolysis in response to PEFinduced cell death (Silve et al, 2018a;Jaeschke et al, 2019;Scherer et al, 2019). Therefore, effects on short and on long time scales should be considered for the goal of improving the efficiency of microalgae biorefining by applying PEF technology.…”

Section: Discussion Of Kinetic Modeling and Numerical Simulation As Tmentioning

confidence: 99%

Kinetic Modeling and Numerical Simulation as Tools to Scale Microalgae Cell Membrane Permeabilization by Means of Pulsed Electric Fields (PEF) From Lab to Pilot Plants

Knappert

McHardy

Rauh

2020

Front. Bioeng. Biotechnol.

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Pulsed Electric Fields (PEF) is a promising technology for the gentle and energy efficient disruption of microalgae cells such as Chlorella vulgaris. The technology is based on the exposure of cells to a high voltage electric field, which causes the permeabilization of the cell membrane. Due to the dependency of the effective treatment conditions on the specific design of the treatment chamber, it is difficult to compare data obtained in different chambers or at different scales, e.g., lab or pilot scale. This problem can be overcome by the help of numerical simulation since it enables the accessibility to the local treatment conditions (electric field strength, temperature, flow field) inside a treatment chamber. To date, no kinetic models for the cell membrane permeabilization of microalgae are available what makes it difficult to decide if and in what extent local treatment conditions have an impact on the permeabilization. Therefore, a kinetic model for the perforation of microalgae cells of the species Chlorella vulgaris was developed in the present work. The model describes the fraction of perforated cells as a function of the electric field strength, the temperature and the treatment time by using data which were obtained in a milliliter scale batchwise treatment chamber. Thereafter, the model was implemented in a CFD simulation of a pilot-scale continuous treatment chamber with colinear electrode arrangement. The numerical results were compared to experimental measurements of cell permeabilization in a similar continuous treatment chamber. The predicted values and the experimental data agree reasonably well what demonstrates the validity of the proposed model. Therefore, it can be applied to any possible treatment chamber geometry and can be used as a tool for scaling cell permeabilization of microalgae by means of PEF from lab to pilot scale. The present work provides the first contribution showing the applicability of kinetic modeling and numerical simulation for designing PEF processes for the purpose of biorefining microalgae biomass. This can help to develop new processes and to reduce the costs for the development of new treatment chamber designs.

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Section: Discussion Of Kinetic Modeling and Numerical Simulation As Tmentioning

confidence: 99%

Kinetic Modeling and Numerical Simulation as Tools to Scale Microalgae Cell Membrane Permeabilization by Means of Pulsed Electric Fields (PEF) From Lab to Pilot Plants

Knappert

McHardy

Rauh

2020

Front. Bioeng. Biotechnol.

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“…Silve et al. () incubated PEF‐treated cells of Auxenochlorella protothecoides in aqueous media under inert conditions, thereby enhancing the efficiency of the subsequent extraction of lipids in solvents while reducing specific treatment energy. Almost total extraction was achieved after a 20 hr incubation period at 25 °C, while incubation on ice was still beneficial but less efficient than at 25 °C.…”

Section: Pulsed Electric Fields Technology For Enhancing Extraction Omentioning

confidence: 99%

“…And, more recently, Gonçalves, Alvim-Ferraz, Martins, Simões, and Pires (2016) evaluated PEF for the collecting of lipids in an economically feasible method associated with microalgae cultivation in wastewater. Silve et al (2018) incubated PEF-treated cells of Auxenochlorella protothecoides in aqueous media under inert conditions, thereby enhancing the efficiency of the subsequent extraction of lipids in solvents while reducing specific treatment energy. Almost total extraction was achieved after a 20 hr incubation period at 25 • C, while incubation on ice was still beneficial but less efficient than at 25 • C. They suggested that the spontaneous release of ions and carbohydrates due to electroporation facilitated subsequent successful lipid extraction, although a direct causality between the two phenomena was not demonstrated.…”

Section: Pef-assisted Extraction From Microalgaementioning

confidence: 99%

Pulsed electric field‐assisted extraction of valuable compounds from microorganisms

Martínez

Delso

Álvarez

et al. 2020

Comp Rev Food Sci Food Safe

113

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Microorganisms (bacteria, yeast, and microalgae) are a promising resource for products of high value such as nutrients, pigments, and enzymes. The majority of these compounds of interest remain inside the cell, thus making it necessary to extract and purify them before use. This review presents the challenges and opportunities in the production of these compounds, the microbial structure and the location of target compounds in the cells, the different procedures proposed for improving extraction of these compounds, and pulsed electric field (PEF)‐assisted extraction as alternative to these procedures. PEF is a nonthermal technology that produces a precise action on the cytoplasmic membrane improving the selective release of intracellular compounds while avoiding undesirable consequences of heating on the characteristics and purity of the extracts. PEF pretreatment with low energetic requirements allows for high extraction yields. However, PEF parameters should be tailored to each microbial cell, according to their structure, size, and other factors affecting efficiency. Furthermore, the recent discovery of the triggering effect of enzymatic activity during cell incubation after electroporation opens up the possibility of new implementations of PEF for the recovery of compounds that are bounded or assembled in structures. Similarly, PEF parameters and suspension storage conditions need to be optimized to reach the desired effect. PEF can be applied in continuous flow and is adaptable to industrial equipment, making it feasible for scale‐up to large processing capacities.

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“…Cells were harvested by centrifugation at 10000 ×g, 12 min at 10 °C. Since the high initial conductivity of the suspension at 22 mS•cm -1 can increase the energy requirement of PEF treatment (Silve et al, 2018), cells were washed using sodium-phosphate buffer pH 7.2 at 0.1 mS•cm -1 , in order to reduce the conductivity of the cyanobacterial suspension. The conductivity of the suspension was adjusted to 1.7 mS•cm -1 using sodium-phosphate buffer and the medium.…”

Section: Cyanobacteria Biomassmentioning

confidence: 99%

Impact of incubation conditions on protein and C-Phycocyanin recovery from Arthrospira platensis post- pulsed electric field treatment

Akaberi

Krust

Müller

et al. 2020

Bioresource Technology

Self Cite

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Pulsed electric field (PEF) was conducted for the extraction of proteins/C-Phycocyanins from Arthrospira platensis. The cyanobacterial suspension was treated with 1 µs long pulses at an electric field strength of 40 kV•cm-1 and a treatment energy of 114 kJ•kgsus −1 and 56 kJ•kgsus −1. For benchmarking, additional biomass was processed by high pressure homogenization. Homogeneity of the suspension prior to PEF-treatment influenced the protein/C-phycocyanin extraction efficiency. Stability of C-Phycocyanin during post-PEF incubation time was affected by incubation temperature and pH of the external medium. Biomass concentration severely affect proteins/C-Phycocyanins extraction yield via PEF-treatment. The optimum conditions for extraction of proteins/C-Phycocyanin was obtained at 23 °C while incubating in pH 8-buffer. The energy demand for PEF-treatment amounts to 0.56 MJ•kgdw-1 when processing biomass at 100 gdw•kgsus −1. PEF treatment enhances the C-Phycocyanin purity ratio, thus, it can be suggested as preferential downstream processing method for the production of C-Phycocyanin from A. platensis biomass.

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Incubation time after pulsed electric field treatment of microalgae enhances the efficiency of extraction processes and enables the reduction of specific treatment energy

Cited by 48 publications

References 41 publications

Kinetic Modeling and Numerical Simulation as Tools to Scale Microalgae Cell Membrane Permeabilization by Means of Pulsed Electric Fields (PEF) From Lab to Pilot Plants

Kinetic Modeling and Numerical Simulation as Tools to Scale Microalgae Cell Membrane Permeabilization by Means of Pulsed Electric Fields (PEF) From Lab to Pilot Plants

Pulsed electric field‐assisted extraction of valuable compounds from microorganisms

Impact of incubation conditions on protein and C-Phycocyanin recovery from Arthrospira platensis post- pulsed electric field treatment

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