Incremento De La Expresión De Tlr4 Y Efecto Antioxidante Del Ácido Acetilsalicílico en Conejos Con Dieta Alta en Grasas

Abstract

RESUMENIntroducción: La obesidad y el desarrollo de enfermedades hepáticas que se caracterizan por el aumento y acumulación de lípidos en tejidos y sangre, inflamación y estrés oxidante, son actualmente una epidemia mundial, y en la población mexicana es cada vez mayor el número de jóvenes afectados. Este aumento ha conducido a la investigación médica hacia la detección temprana del síndrome metabólico, que se emplea como indicador de síntomas que pueden ser de riesgo para la salud y conducir a enfermedades hepáticas. Objetivo: Los objetivos del presente trabajo fueron: evaluar en un modelo de dislipidemia en conejos jóvenes alimentados con una dieta alta en grasa (ácido palmítico al 20%), la producción de especies reactivas del oxígeno y cambios en la expresión de genes TLR4, COX2y de !L-1β como marcadores de inflamación y de estrés oxidante, así como evaluar el efecto del ácido acetilsalicílico en la producción de radicales libres y en la expresión de estos genes. Resultados: En los conejos alimentados con exceso de grasa aumentaron los niveles de triglicéridos (p<0.05), la expresión de TLR4 y las especies reactivas del oxígeno, aunque éstas últimas no de forma significativa. La administración de ácido acetilsalicílico en dosis antiinflamatorias disminuyó la producción de especies reactivas del oxígeno y la expresión de TLR4. Discusión: La ingesta elevada de grasa en conejos jóvenes por un período corto de tiempo conduce a la dislipidemia y a la sobreexpresión de TLR4, gen clave de la respuesta inflamatoria y vinculada al aumento de las especies reactivas del oxígeno. Los resultados indican que el ácido acetilsalicílico tiene efecto antioxidante. Palabras Clave: Obesidad, dislipidemia, inflamación, estrés oxidante, hígado graso no alcohólico (HGNA). ABSTRACT Introduction:The obesity and liver diseases progression are characterized by the increase and accumulation of lipids in tissues and blood, inflammation and oxidative stress. These diseases are now a worldwide epidemic, and the number of young people affected is increasing in the Mexican population. This increase has led to medical research towards the early detection of the metabolic syndrome, which is used as an indicator of symptoms that may be at risk for health and lead to liver disease. Objective: The objectives of the present study were to evaluate in a model of dyslipidemia in young rabbits fed three months with a diet high in fat (20% palmitic acid), the production of reactive oxygen species and changes in TLR4, COX2 and !L-1β gene expression, as markers of inflammation and oxidative stress; also to evaluate the effect of acetylsalicylic acid on the production of free radicals and on the expression of these genes. Results: In rabbits fed with excess of fat, significantly increased the levels of triglycerides (p<0.05), TLR4 expression, and reactive oxygen species, although in the latter, not significantly. The administration of acetylsalicylic acid in anti-inflammatory doses decreased the production of reactive oxygen species and the expression of TL...

Denitrase activity of Debaryomyces�hansenii reduces the oxidized compound 3‑nitrotyrosine in mice liver with colitis

“…In the present study, we provided evidence that colitis can affect other organs such as liver, given that we observed an increase of ROS production in the crude extracts of livers from mice with colitis. In this respect, research is scarce, due to the complexity of evaluating that a disease such as colitis or obesity in animal models lead to oxidative stress condition in the liver (16,34).…”

“…The cascade of TLR4 can induce alterations of the mitochondrial function and thus the increase of ROS production. Also, during the consumption of fat-rich diets, it has been shown there is an overexpression of TLR4 gene (15) and production of ROS (16).…”

Inducción y evaluación del síndrome metabólico en modelo animal: revisión bibliográfica

“…The effects of diets rich in different fatty acids using lard, shear butter, olive oil, safflower oil and soybean oil have been studied in mice, the diets were designed to resemble human diets, results showed differences in body weight after twelve weeks, the animals consuming diets with soybean oil and lard [15]. The use of 20% palmitic acid in the feeding of young rabbits has shown an increase in triglyceride levels, cholesterol levels, as well as the presence of dyslipidaemia, a condition in which oxidative stress due to peroxidation of lipids has been reported, when there is an overproduction of reactive oxygen species and the capacity of antioxidant systems is exceeded, an alteration in cellular functions is generated later organs and tissues [5].…”

“…Therefore, to determine the presence of dyslipidaemia in young fat-fed rabbits, lipid profiles have been obtained comparing the average values of these in animals fed a normal, fat-free diet and in rabbits fed a high fat diet, elevated triglyceride levels and a decrease in HDL being the most indicative of the presence of MS [5].…”