Increasing the efficiency of recurrent selection for angular leaf spot resistance in common bean

Librelon, Samira Santiago; Pádua, Paula Furtado de; Abreu, Ângela de Fátima Barbosa; Ramalho, Magno Antônio Patto; Souza, Elaine Aparecida de

doi:10.1002/csc2.20010

Cited by 11 publications

(14 citation statements)

References 33 publications

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“…Regarding estimated heritability, similar values were reported by Oblessuc et al (2012), who estimated h 2 ranging from 0.51 to 0.81 for ALS resistance evaluated in different environments. Bassi et al (2017) reported similar heritability for the AS population in assessing resistance to ALS at the V3 stage (h 2 = 0.62), and Librelon et al (2020) as well for the recurrent selection progenies evaluated in the V2 stage (h 2 = 0.64). A smaller number of genotypes were considered resistant in evaluation in the V2 stage, and it may be considered a phenological stage for moderate selection.…”

Section: Degrees Of Resistance Throughout the Plant Growthmentioning

confidence: 77%

“…While only 5 loci have been reported by the BIC Genetics Committee, several authors have detected quantitative control for the disease, and numerous QTL have already been identified, showing the complex inheritance of ALS resistance (López et al, 2003;Caixeta et al, 2005;Oblessuc et al, 2012;Keller et al, 2015;Perseguini et al, 2016;Bassi et al, 2017;Pereira L. A. et al, 2019Librelon et al, 2020). In a recent study, bean accessions evaluated in three PGS and environments regarding degree of resistance to ALS exhibited different resistance levels, as the same cultivar considered resistant in one PGS showed susceptibility in another (Pereira R. et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Angular Leaf Spot Resistance Loci Associated With Different Plant Growth Stages in Common Bean

et al. 2021

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Angular leaf spot (ALS) is a disease that causes major yield losses in the common bean crop. Studies based on different isolates and populations have already been carried out to elucidate the genetic mechanisms of resistance to ALS. However, understanding of the interaction of this resistance with the reproductive stages of common bean is lacking. The aim of the present study was to identify ALS resistance loci at different plant growth stages (PGS) by association and linkage mapping approaches. An BC2F3 inter-gene pool cross population (AND 277 × IAC-Milênio – AM population) profiled with 1,091 SNPs from genotyping by sequencing (GBS) was used for linkage mapping, and a carioca diversity panel (CDP) genotyped by 5,398 SNPs from BeadChip assay technology was used for association mapping. Both populations were evaluated for ALS resistance at the V2 and V3 PGSs (controlled conditions) and R8 PGS (field conditions). Different QTL (quantitative trait loci) were detected for the three PGSs and both populations, showing a different quantitative profile of the disease at different plant growth stages. For the three PGS, multiple interval mapping (MIM) identified seven significant QTL, and the Genome-wide association study (GWAS) identified fourteen associate SNPs. Several loci validated regions of previous studies, and Phg-1, Phg-2, Phg-4, and Phg-5, among the 5 loci of greatest effects reported in the literature, were detected in the CDP. The AND 277 cultivar contained both the Phg-1 and the Phg-5 QTL, which is reported for the first time in the descendant cultivar CAL143 as ALS10.1UC. The novel QTL named ALS11.1AM was located at the beginning of chromosome Pv11. Gene annotation revealed several putative resistance genes involved in the ALS response at the three PGSs, and with the markers and loci identified, new specific molecular markers can be developed, representing a powerful tool for common bean crop improvement and for gain in ALS resistance.

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Section: Degrees Of Resistance Throughout the Plant Growthmentioning

confidence: 77%

Section: Introductionmentioning

confidence: 99%

Angular Leaf Spot Resistance Loci Associated With Different Plant Growth Stages in Common Bean

et al. 2021

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“…In addition to these biparental crosses, another alternative would be genetic improvement by means of recurrent selection of populations developed from multiple crosses among these cultivars. Recurrent selection proved to be an effective method for improving common beans for seed yield and several other traits of interest (Librelon, Pádua, Abreu, Ramalho, & Souza, 2020;Lopes et al, 2019;Ramalho, Abreu, & dos Santos, 2005;Singh, Terán, Muñoz, & Takegami, 1999). Although there is few information on the genetic improvement of common beans for NUsE, several studies have reported genetic progress for NUsE and low-N tolerance using recurrent selection in other crops (Bukan, Šarčević, Gunjača, Bolarić, & Kozumplik, 2009;Lafitte et al, 1994;Ranjan & Yadav, 2019;Udo, Ajala, & Olaniyan, 2017).…”

Section: Crop Sciencementioning

confidence: 99%

Genetic diversity among Brazilian carioca common bean cultivars for nitrogen use efficiency

et al. 2021

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The objective of this study was to determine the genetic diversity among Brazilian carioca common bean (Phaseolus vulgaris L.) cultivars for traits related to nitrogen use efficiency (NUsE). An additional objective was to identify potential parents and promising parental combinations to assist common bean breeding programs in the development of cultivars with improved NUsE. The experiments were carried out at the Experimental Stations of the Instituto de Desenvolvimento Rural do Paraná (IDR-Paraná) in Londrina and Ponta Grossa, Brazil, during the rainy season in 2017. Thirty-nine common bean cultivars were evaluated under high (40 kg N ha −1 ) and low N (0 kg N ha −1 ) applied as top-dressing. The cultivars were genotyped using the genotyping-by-sequencing (GBS) approach with restriction enzyme CviAII. A total of 23,823 single nucleotide polymorphism (SNP) markers were identified throughout the common bean genome. A wide genetic variability was observed for NUsErelated traits under high and low N conditions. In addition, correlations were found between shoot dry biomass and NUsE, N uptake efficiency (NUpE), and N utilization efficiency (NUtE) under low N, indicating the possibility of indirect selection of these traits by the plant shoot dry biomass. Interestingly, the most low-N-tolerant cultivars were not necessarily the most responsive and/or NUsE, suggesting that different morphophysiological mechanisms control these traits. The cultivars that stood out positively in this study can be used as parents in breeding programs aimed at developing cultivars that are adapted to N limitation.

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“…Angular leaf spot, caused by the fungus Pseudocercospora griseola (Sacc.) Crous and U. Braun, is one of the most serious diseases in common beans; it may cause yield losses of up to 80% (Nay et al, 2019a;Librelon et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Precise assessment of angular leaf spot severity using the Leaf Doctor app for common beans

Almeida¹,

Bonfante²,

Paulino³

et al. 2021

Afr. J. Biotechnol.

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Angular leaf spot (ALS) is one of the most severe diseases of common bean. However, there is as yet no standardization of the phenological stage, inoculum concentration, diagrammatic scale (DS), and day of assessment for screening ALS resistance in bean lines. The present study aimed to evaluate the potential for assessment of ALS severity in common beans using the Leaf Doctor app and inoculation at the V2 phenological stage under two inoculation concentrations. The experiment was carried out in a completely randomized design in a 3 × 2 × 2 factorial arrangement with three cultivars (AND 277, IAC-Milênio, and IAC-Carioca), two monosporic isolates (31-31 and 10-59), and two inoculum concentrations (2 × 10 4 and 4 × 10 4 conidia/mL). Disease severity was evaluated from 11 to 15 days after inoculation (d.a.i) using a diagrammatic scale and the Leaf Doctor. The results showed that 15 d.a.i., the concentration of the inoculum was no longer significant and that the isolates showed no significant difference in terms of pathogenicity. Moreover, in 15 d.a.i. it was possible to separate the cultivars into three phenotypic classes: resistant (AND-277), moderately susceptible (IAC-Milênio) and susceptible (IAC-Carioca). Considering the diagrammatic scale of notes, the cultivar AND 277 had a score of 2.3, while for the image evaluation, it was observed that only 0.65% of the total leaf area was affected by the disease. For this reason, 15 d.a.i. was recommended as the better day for evaluation, and the concentration of 2 × 10 4 conidia/mL may be adopted in order to reduce the amount of inoculum needed for the experiment. In addition, the evaluation with the Leaf Doctor app increases accuracy and improves discrimination between resistant and susceptible cultivars.

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Increasing the efficiency of recurrent selection for angular leaf spot resistance in common bean

Cited by 11 publications

References 33 publications

Angular Leaf Spot Resistance Loci Associated With Different Plant Growth Stages in Common Bean

Angular Leaf Spot Resistance Loci Associated With Different Plant Growth Stages in Common Bean

Genetic diversity among Brazilian carioca common bean cultivars for nitrogen use efficiency

Precise assessment of angular leaf spot severity using the Leaf Doctor app for common beans

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