Increasing PHB production with an industrially scalable hardwood hydrolysate as a carbon source

Dietrich, Karolin; Oliveira-Filho, Edmar R.; Dumont, Marie‐Josée; Gomez, José Gregório Cabrera; Taciro, Marilda Keico; Silva, Luiziana Ferreira da; Orsat, Valérie; Rio, Luis F. Del

doi:10.1016/j.indcrop.2020.112703

Cited by 39 publications

(21 citation statements)

References 30 publications

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“…To overcome the death valley from research scale to market, we recently reported the production of PHB with wood hydrolysate from a pilot‐scale forest biorefinery. Forest biorefineries convert the cellulose, hemicellulose, and lignin into products, and in this case, PHB production is coupled to the recovery of a highly pure lignin fraction 10 . The process provides scalable amounts of sustainable PHB, while also optimizing the material recovery of the whole biomass feedstock.…”

Section: Introductionmentioning

confidence: 99%

“…Since the carbon source has the highest impact on the PHB price in industrial scale production, here the material properties of a PHB made from a promising carbon source were studied. The carbon source was a wood hydrolysate (WH) produced in a pilot scale forest biorefinery 10 . The material properties were compared with a PHB produced with a synthetic sugar solution (SH) 24 .…”

Section: Introductionmentioning

confidence: 99%

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In‐depth material characterization of polyhydroxybutyrate from a forest biorefinery

Dietrich

Dumont

Orsat

et al. 2021

J of Applied Polymer Sci

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Large-scale replacement of petroplastics with compostable plastics, like polyhydroxybutyrates (PHB) will contribute to elimination plastic pollution, decrease greenhouse gas emissions, and valorize local biomass resources. Lignocellulose hydrolysates have emerged as potentially sustainable carbon sources for PHB production. For industrial processing, it is necessary to know the polymer properties. Yet, most studies on PHB samples from lignocellulose report few material properties. PHB samples produced from a pilot scale hardwood holocellulose hydrolysate conversion process were characterized and compared with PHB from a sugar hydrolysate and a commercial PHB powder. PHB from hardwood holocellulose hydrolysate was found to be comparable with commercial PHB in all properties. Differential scanning calorimetry and thermal gravimetric analysis showed that all samples had similar thermal behavior, where the melting temperature was 176 C and the decomposition temperature was 293 C. From the melting enthalpy, all samples showed 63% crystallinity. Dynamic mechanical analysis showed a glass transition temperature at 5 C and a crystallization temperature of 57 C. Fourier transform infrared spectroscopy and nuclear magnetic resonance confirmed that the samples were homopolymers comprised of hydroxybutyrate units. The difference among the samples was the number average molecular mass, being lower for wood hydrolysate (246.4 kDa) than sugar hydrolysate (670.3 kDa).

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In‐depth material characterization of polyhydroxybutyrate from a forest biorefinery

Dietrich

Dumont

Orsat

et al. 2021

J of Applied Polymer Sci

Self Cite

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“…These authors compared a synthetic hydrolysate that consisted of equivalent substrates (acetate, xylose, glucose) with a wood hydrolysate. PHB formation was performed during 52 h HCDCs [ 219 ]. Interestingly, the PHB concentration (22 g/L) was relatively lower using the synthetic hydrolysate medium.…”

Section: Industrial/agro-industrial Waste For Production Of Scl-and Mcl-phamentioning

confidence: 99%

“…This can be explained by the presence of additional nutrients in wood hydrolysate and the corresponding specific growth rates that were higher on the wood hydrolysate (0.36 vs. 0.33 h −1 ). The cultivation on wood hydrolysate, the total biomass, %-PHB in the CDW and an overall productivity were 59.5 g/L, 58% and 0.72 g/L/h, respectively [ 219 ]. In comparison, the synthetic hydrolysate resulted in %-PHB in the CDW and an overall productivity of 55% and 0.46 g/L/h, respectively [ 219 ].…”

Section: Industrial/agro-industrial Waste For Production Of Scl-and Mcl-phamentioning

confidence: 99%

“…The cultivation on wood hydrolysate, the total biomass, %-PHB in the CDW and an overall productivity were 59.5 g/L, 58% and 0.72 g/L/h, respectively [ 219 ]. In comparison, the synthetic hydrolysate resulted in %-PHB in the CDW and an overall productivity of 55% and 0.46 g/L/h, respectively [ 219 ]. This report on the conversion of hard wood hydrolysate is promising but, as in the above work by Sathiyanarayanan et al on PIW, further process and potentially cell engineering will be needed to achieve industrially relevant product titers and productivity.…”

Section: Industrial/agro-industrial Waste For Production Of Scl-and Mcl-phamentioning

confidence: 99%

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Emergent Approaches to Efficient and Sustainable Polyhydroxyalkanoate Production

2021

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Petroleum-derived plastics dominate currently used plastic materials. These plastics are derived from finite fossil carbon sources and were not designed for recycling or biodegradation. With the ever-increasing quantities of plastic wastes entering landfills and polluting our environment, there is an urgent need for fundamental change. One component to that change is developing cost-effective plastics derived from readily renewable resources that offer chemical or biological recycling and can be designed to have properties that not only allow the replacement of current plastics but also offer new application opportunities. Polyhydroxyalkanoates (PHAs) remain a promising candidate for commodity bioplastic production, despite the many decades of efforts by academicians and industrial scientists that have not yet achieved that goal. This article focuses on defining obstacles and solutions to overcome cost-performance metrics that are not sufficiently competitive with current commodity thermoplastics. To that end, this review describes various process innovations that build on fed-batch and semi-continuous modes of operation as well as methods that lead to high cell density cultivations. Also, we discuss work to move from costly to lower cost substrates such as lignocellulose-derived hydrolysates, metabolic engineering of organisms that provide higher substrate conversion rates, the potential of halophiles to provide low-cost platforms in non-sterile environments for PHA formation, and work that uses mixed culture strategies to overcome obstacles of using waste substrates. We also describe historical problems and potential solutions to downstream processing for PHA isolation that, along with feedstock costs, have been an Achilles heel towards the realization of cost-efficient processes. Finally, future directions for efficient PHA production and relevant structural variations are discussed.

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A review of Canadian wood conversion technologies for the production of fuels and chemicals

et al. 2023

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Canada has 347 million ha of forest cover, contributing to the potential large availability of wood‐based resources. Although Canada's forest sector contributed $23.7 billion to the national nominal gross domestic product (GDP) in 2019, the GDP contribution of the wood product manufacturing subsector shrank by 6%. To reposition the Canadian forest industry, new forest management practices and wood‐based conversion technologies should be applied. In this context, the use of woody biomass in biorefineries to produce clean energy, fuels, and chemicals is becoming increasingly significant. There is a need to understand the current status and challenges of the wood‐based biomass conversion technologies that have been and are being developed in Canada. This information will help decision‐makers in formulating and implementing forest sector‐related policies for a sustainable bioeconomy in Canada. This study is focused on a review of Canadian woody biomass conversion technologies. Our critical review identified considerable potential biomass conversion technologies specialized for woody feedstock, all in the Canadian setting. We focused on the prospects of revitalizing Canada's pulp and paper industry through the integration of pre‐treatment processes and biochemical technologies. The thermochemical conversion pathway was identified as the dominant route for woody feedstock valorization. The review also identified pathways with the potential to diversify the existing product mix that generate products from wood streams, such as chemicals and biomaterials. Most of the biochemical and thermochemical research done in institutional and multi‐institutional research collaborations from laboratory scale to industrial scale will boost the chances of the commercialization of a wood‐based biorefinery in Canada.

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Increasing PHB production with an industrially scalable hardwood hydrolysate as a carbon source

Cited by 39 publications

References 30 publications

In‐depth material characterization of polyhydroxybutyrate from a forest biorefinery

In‐depth material characterization of polyhydroxybutyrate from a forest biorefinery

Emergent Approaches to Efficient and Sustainable Polyhydroxyalkanoate Production

A review of Canadian wood conversion technologies for the production of fuels and chemicals

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