Increasing litter size in a sheep breed by marker-assisted selection of BMPR1B A746G mutation

Sun, Hongxin; Tian, Shujun; Xiang, Hai; Zhou, Liansheng; Dun, Weitao; Zhao, Xingbo

doi:10.1007/s12041-015-0470-8

Cited by 9 publications

(9 citation statements)

References 15 publications

(13 reference statements)

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“…With deep research into high-fecundity genes in sheep, the FecB gene has been increasingly applied to the cultivation of new sheep varieties. For example, Chen et al (2015) used the FecB effect to cross small-tail Han (STH) sheep with Dorper sheep, and the average litter size in the hybrid offspring was significantly higher than in Dorper sheep (P < 0.05). CRISPR/Cas9 technology has also been applied to sheep embryos, establishing a technical basis for editing the sheep BMPR1B gene (Zhang et al, 2017;Rui et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

The expression and mutation of BMPR1B and its association with litter size in small-tail Han sheep (Ovis aries)

Wen

Guo

et al. 2021

Arch. Anim. Breed.

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Abstract. Previous studies have shown that BMPR1B promotes follicular development and ovarian granulosa cell proliferation, thereby affecting ovulation in mammals. In this study, the expression and polymorphism of the BMPR1B gene associated with litter size in small-tail Han (STH) sheep were determined. The expression of BMPR1B was detected in 14 tissues of STH sheep during the follicular phase as well as in the hypothalamic–pituitary–gonadal (HPG) axis of monotocous and polytocous STH sheep during the follicular and luteal phases using quantitative polymerase chain reaction (qPCR). Sequenom MassARRAY® single nucleotide polymorphism (SNP) technology was also used to detect the polymorphism of SNPs in seven sheep breeds. Here, BMPR1B was highly expressed in hypothalamus, ovary, uterus, and oviduct tissue during the follicular phase, and BMPR1B was expressed significantly more in the hypothalamus of polytocous ewes than in monotocous ewes during both the follicular and luteal phases (P<0.05). For genotyping, we found that genotype and allele frequencies of three loci of the BMPR1B gene were extremely significantly different (P<0.01) between the monotocous and polytocous groups. Association analysis results showed that the g.29380965A>G locus had significant negative effects on the litter size of STH sheep, and the combination of g.29380965A>G and FecB (Fec – fecundity and B – Booroola; A746G) at the BMPR1B gene showed that the litter size of AG–GG, AA–GG, and GG–GG genotypes was significantly higher compared with other genotypes (P<0.05). This is the first study to find a new molecular marker affecting litter size and to systematically analyze the expression of BMPR1B in different fecundity and physiological periods of STH sheep.

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Section: Introductionmentioning

confidence: 99%

The expression and mutation of BMPR1B and its association with litter size in small-tail Han sheep (Ovis aries)

Wen

Guo

et al. 2021

Arch. Anim. Breed.

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“…The results of the current meta-analysis were consistent with Fogarty (2009) conclusions that the FecB gene increases the litter size, but he stated that with the rst copy of this gene the litter size increased by 0.7. The nding of the study can be clari ed in particular, assuming that the FecB gene is not the only factor that controls the litter size, it includes many other factors, such as correlative functional genes, maternal inputs and environmental effects (Davis et al, 2006;Chen et al, 2015). Also, the ndings of the current meta-analysis study suggest that certain other sheep breeding molecular markers, including the BMP15, GDF9 genes and others, will also have to be assessed.…”

Section: Discussionmentioning

confidence: 99%

A Meta-analysis Study of Association Between FecB Polymorphism and Litter Size in Sheep

Barazandeh

Mokhtari

Roudbari

et al. 2021

Preprint

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Litter size is perhaps the main commercial trait since it has an observable effect on benefit in the sheep industry. Fecundity genes, play a vital role in expression of litter size. One of the most popular of these genes is the Booroola gene (FecB). In many past researches there was a dependency between the BMPR1B gene polymorphism and litter size of sheep. In the current study, a meta-analysis directed by coalescing outcoming of 9902 cases of 26 published research wherein various breeds of sheep to assess the influence of the FecB gene on litter size utilizing additive, recessive, dominant, and co-dominant genetic models. The random effects model was used for data analysis according to the Cochran Q test and I2 quantity. Under additive (SMD = 0.528), dominant (SMD = 0.468) and recessive (SMD = 0.250) models, the significant effect (P<0.01) of FecB genotypes has been identified. Furthermore, under the co-dominant (SMD = -0.050, P = 0.3332) model, the association between FecB genotypes and litter size trait had not been detected. A growth in litter size by about 0.47 lambs (Dominant model) was associated with the first copy of the FecB gene and 0.25 lambs (Recessive model) with the second copy of this gene. Consequences of the current study support the idea that BMPR1B fundamentally influenced litter size and subsequently it may be utilized for Marker-assisted selection programmers for improved genetic merit of reproductive futures and furthermore insert this gene by crossbreeding in low prolific breeds may improve reproductive characteristics.

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“…The point mutation (A turn to G) at base 746 of the coding region of BMPR‐1B, changing a glutamine to an arginine, is associated with the hyperprolific profile of Booroola ewes (Souza et al., 2001; Wilson et al., 2001). Thus, the FecB mutation has become one of the candidates for breeding sheep for high prolificacy using marker‐assisted selection (Chen et al., 2015; Hua & Yang, 2009).…”

Section: Introductionmentioning

confidence: 99%

“…Despite the thriving sheep farming in the Xilingol region, SNPs in the FecB locus remain unknown in the above three breeds. The hyperprolific effect of the FecB mutation can serve as a genetic marker in marker‐assisted selection strategies to increase litter size of sheep (Chen et al., 2015; Wang et al., 2018). SNPs in the FecB locus are usually identified using PCR‐RFLP (Chu et al., 2007; Mahdavi et al., 2014), PCR‐SSCP (Chu et al., 2011; Mulsant et al., 2001), pyrosequencing (Souza et al., 2001), ARMS PCR (Ahlawat et al., 2014), the KASPar method (Wang et al., 2018), and Taq Man real‐time PCR (Woodward, 2014).…”

Section: Introductionmentioning

confidence: 99%

Identifying FecB genotypes in the muscle from sheep breeds indigenous to Xilingol, and establishment of a TaqMan real‐time PCR technique to distinguish FecB alleles

Guo¹,

Li²,

Liu³

et al. 2022

Food Science & Nutrition

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The muscle from Xilingol indigenous sheep breeds are famous in China, and the FecB genotype in this population remains uncharacterized. In this study, SNPs in the FecB locus were investigated by pyrosequencing, and an optimized PCR‐RFLP technique was generated to identify SNPs. In addition, an efficient technique for high‐throughput identification of SNPs in FecB was optimized using TaqMan real‐time PCR and breed‐conservative primers and SNP‐specific probes. By genotyping the FecB locus in the muscle of Xilingol indigenous sheep breeds using a novel TaqMan real‐time PCR assay, our study has generated the groundwork for the authentication of Xilingol mutton based on the specific gene and the prolificacy‐oriented breeding of Xilingol sheep using marker‐assisted selection strategies in the future.

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Increasing litter size in a sheep breed by marker-assisted selection of BMPR1B A746G mutation

Abstract: 2015 Increasing litter size in a sheep breed by marker-assisted selection of BMPR1B A746G mutation. J. Genet. 94, xx-xx]

Cited by 9 publications

References 15 publications

The expression and mutation of <i>BMPR1B</i> and its association with litter size in small-tail Han sheep (<i>Ovis aries</i>)

The expression and mutation of <i>BMPR1B</i> and its association with litter size in small-tail Han sheep (<i>Ovis aries</i>)

A Meta-analysis Study of Association Between FecB Polymorphism and Litter Size in Sheep

Identifying FecB genotypes in the muscle from sheep breeds indigenous to Xilingol, and establishment of a TaqMan real‐time PCR technique to distinguish FecB alleles

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Increasing litter size in a sheep breed by marker-assisted selection of BMPR1B A746G mutation

Abstract: 2015 Increasing litter size in a sheep breed by marker-assisted selection of BMPR1B A746G mutation. J. Genet. 94, xx-xx]

Cited by 9 publications

References 15 publications

The expression and mutation of &lt;i&gt;BMPR1B&lt;/i&gt; and its association with litter size in small-tail Han sheep (&lt;i&gt;Ovis aries&lt;/i&gt;)

The expression and mutation of &lt;i&gt;BMPR1B&lt;/i&gt; and its association with litter size in small-tail Han sheep (&lt;i&gt;Ovis aries&lt;/i&gt;)

A Meta-analysis Study of Association Between FecB Polymorphism and Litter Size in Sheep

Identifying FecB genotypes in the muscle from sheep breeds indigenous to Xilingol, and establishment of a TaqMan real‐time PCR technique to distinguish FecB alleles

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The expression and mutation of <i>BMPR1B</i> and its association with litter size in small-tail Han sheep (<i>Ovis aries</i>)

The expression and mutation of <i>BMPR1B</i> and its association with litter size in small-tail Han sheep (<i>Ovis aries</i>)