The expression and mutation of &amp;lt;i&amp;gt;BMPR1B&amp;lt;/i&amp;gt; and its association with litter size in small-tail Han sheep (&amp;lt;i&amp;gt;Ovis aries&amp;lt;/i&amp;gt;)

Wen, Yuliang; Guo, Xiaofei; Ma, Lin; Zhang, Xiaosheng; Zhang, Jinlong; Zhao, Shengguo; Chu, Mingxing

doi:10.5194/aab-64-211-2021

Cited by 31 publications

(19 citation statements)

References 53 publications

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“…In addition, the FecB mutation was absent in Thoka, Woodlands, Olkuska, Lacaune, Belclare, and Cambridge sheep breeds [32] This FecB mutation effects not only on litter size trait but also reported to have effects on other traits related with reproductive efficiency of ewes such as fertility and embryo survival, lamb survival, lamb growth, carcass quality, wool production that supports the present findings [35]. Moreover, the g.29380965A>G locus of BMPR1B gene found to had negative effect on the litter size of STH sheep of China, but combination with FecB genotype produce significantly (P<0.05) higher litter size [36].…”

Section: Discussionsupporting

confidence: 85%

Molecular characterization of multidrug-resistant Escherichia coli isolated from human urine infections with their antibiogram profile

Haque¹,

Islam²,

Miah³

et al. 2023

J Adv Biotechnol Exp Ther

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Urinary tract infections (UTIs) are the leading cause of hospitalization due to bacterial infection, and the frequency of multidrug-resistant Escherichia coli isolates from these infections is increasing worldwide. The current study aims to isolate and characterize antibiotic-resistant Escherichia coli and their antibiogram typing from urine samples of humans. From April to December 2019, a total of 60 human urine samples were collected aseptically and treated to primary isolation by propagation in nutrient broth followed by culture on various agar media. Gram’s staining, string techniques, biochemical characterization, PCR, and Sanger sequencing were performed to confirm E. coli. The Kirby-Bauer disk diffusion technique was used to test the susceptibility of all bacterial strains to thirteen typically prescribed antibiotics. The overall prevalence of E. coli in UTIs was 66.67%. Three variations were noted in E. coli, all of which were single substitutions (A>T, C>T, and T>A). Phylogenetic analysis of the 16S rRNA revealed that the E. coli discovered in this study belonged to the genus Escherichia, but was distinct from those identified in other countries. The antibiograms revealed that all the isolates (100%) were resistant to penicillin, ampicillin, and amoxicillin; 94.87% to doxycycline; 79.16% to gentamycin; 75.48% to ciprofloxacin; 73.07% to erythromycin; 71.66% to levofloxacin; 47.36% to ceftriaxone; and 46.66% to tetracycline. In contrast, all E. coli strains were sensitive to amikacin (95%), vancomycin (92.50%), and azithromycin (92.50%). People with a urinary tract infection (UTI) often have multidrug-resistant E. coli in their urine samples, which calls for a one-health strategy to deal with this rapidly changing condition.

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Section: Discussionsupporting

confidence: 85%

Molecular characterization of multidrug-resistant Escherichia coli isolated from human urine infections with their antibiogram profile

Haque¹,

Islam²,

Miah³

et al. 2023

J Adv Biotechnol Exp Ther

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“…For example, many studies have shown that BMPR1B is associated with litter size in animals [32][33][34]. It is the main gene that affects the ovulation rate and litter size in sheep [35][36][37]. In addition, a series of studies have confirmed that the BMPR1B can affect follicular development, ovulation [38], and cause ovarian insufficiency [39].…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Selective Analysis of Boer Goat to Investigate the Dynamic Heredity Evolution under Different Stages

Yuan

Zhang

Liu

et al. 2022

Animals

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Boer goats, as kemp in meat-type goats, are selected and bred from African indigenous goats under a long period of artificial selection. Their advantages in multiple economic traits, particularly their plump growth, have attracted worldwide attention. The current study displayed the genome-wide selection signature analyses of South African indigenous goat (AF), African Boer (BH), and Australian Boer (AS) to investigate the hereditary basis of artificial selection in different stages. Four methods (principal component analysis, nucleotide diversity, linkage disequilibrium decay, and neighbor-joining tree) implied the genomic diversity changes with different artificial selection intensities in Boer goats. In addition, the θπ, FST, and XP-CLR methods were used to search for the candidate signatures of positive selection in Boer goats. Consequently, 339 (BH vs. AF) and 295 (AS vs. BH) candidate genes were obtained from SNP data. Especially, 10 genes (e.g., BMPR1B, DNER, ITGAL, and KIT) under selection in both groups were identified. Functional annotation analysis revealed that these genes are potentially responsible for reproduction, metabolism, growth, and development. This study used genome-wide sequencing data to identify inheritance by artificial selection. The results of the current study are valuable for future molecular-assisted breeding and genetic improvement of goats.

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“…BMPR1B defects in mice have been shown to cause infertility [ 58 ], and high expression of BMPR1B in ovaries has been related to highly prolific sheep and goats [ 20 , 59 ]. In this study, the SINE-insertion individuals exhibited higher expression of BMPR1B in ovary and greater first litter size in comparison to individuals without SINE insertion.…”

Section: Discussionmentioning

confidence: 99%

“…However, the expression difference was not found in back fat and muscle among these genotypes. It is possible that the expression of BMPR1B in the tissues of brain was relatively high compared with other tissues, including the ovary [ 20 , 23 ], which made it play a major role through modulating the hypothalamic–pituitary axis to affect the growth and development.…”

Section: Discussionmentioning

confidence: 99%

“…Since then, a number of studies have been performed to decipher the relationship between this causal mutation and the prolificacy of sheep or goats [ 15 , 16 , 17 ]. The expression difference among these mutation allele individuals has been identified, in order to further elucidate the mechanism of the A746G mutation on biological processes [ 18 , 19 , 20 ]. The gene editing technology on this point mutation was used to successfully obtain the FecB-mutated sheep [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

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Two Retrotransposon Elements in Intron of Porcine BMPR1B Is Associated with Phenotypic Variation

Chi

Zheng

et al. 2022

Life

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It has been established that through binding to bone morphogenetic proteins (BMPs), bone morphogenetic protein receptor I B (BMPR1B) can mediate transforming growth factor β (TGF-β) signal transduction, and is involved in the regulation of several biological processes, such as bone and muscle formation and homeostasis, as well as folliculogenesis. Also known as FecB, BMPR1B has been reported as the major gene for sheep prolificacy. A number of previous studies have analyzed the relationship between single nucleotide polymorphisms (SNPs) in this gene and its related performance. In recent years, with the illustration of the effect of retrotransposon insertion on the expression of the proximal genes or phenotypic variation, retrotransposon insertion polymorphisms (RIPs) have been used as a novel type of molecular marker in the evaluation of evolution, population structure and breeding of plant and domestic animals. In this study, the RIPs in porcine BMPR1B gene were excavated, and thereafter verified using a comparative genome and polymerase chain reaction (PCR). The potential effects of phenotype, gene expression and functions related to RIPs were also explored. The results showed that 13 distinct RIPs were identified in introns of porcine BMPR1B. Among these, only BMPR1B-SINE-RIP9 and BMPR1B-LINE-RIP13 displayed a close relationship with the growth traits of Large White pigs. Moreover, the total number of BMPR1B-SINE+/+-RIP9 individuals born was found to be significantly higher than that of SINE−/− (p < 0.05). These two RIPs showed an obvious distribution pattern among Chinese indigenous breeds and Western commercial breeds. The expression of BMPR1B in ovaries of adult BMPR1B-SINE+/+-RIP9 Sushan pigs was found to be significantly higher in comparison to those of BMPR1B-SINE−/−-RIP9 (p < 0.05). SINE insertion of BMPR1B-SINE-RIP9 and LINE insertion of BMPR1B-LINE-RIP13 were observed to significantly increase the activity of Octamer binding transcription factor 4 (OCT4) minipromoter in CHO and C2C12 cells (p < 0.01). Therefore, these two RIPs could serve as useful molecular markers for modulating the growth or reproductive traits in assisted selection of pig breeding, while the mechanisms of the insertion function should be studied further.

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The expression and mutation of <i>BMPR1B</i> and its association with litter size in small-tail Han sheep (<i>Ovis aries</i>)

Cited by 31 publications

References 53 publications

Molecular characterization of multidrug-resistant Escherichia coli isolated from human urine infections with their antibiogram profile

Molecular characterization of multidrug-resistant Escherichia coli isolated from human urine infections with their antibiogram profile

Genome-Wide Selective Analysis of Boer Goat to Investigate the Dynamic Heredity Evolution under Different Stages

Two Retrotransposon Elements in Intron of Porcine BMPR1B Is Associated with Phenotypic Variation

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