Increasing electrospun scaffold pore size with tailored collectors for improved cell penetration

Vaquette, Cédryck; Cooper-White, Justin J.

doi:10.1016/j.actbio.2011.02.036

Cited by 222 publications

(186 citation statements)

References 33 publications

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“…Still, not only the size of the surface features matters, but also their aspect ratio, distribution and packing density [53]. Concerning electrospun fibers, aspect ratio doesn't vary with fiber diameter, but to some extent, it is possible to adjust the fiber distribution and density [56]. This could be an additional way of tuning the thrombogenicity of electrospun fibers.…”

Section: Discussionmentioning

confidence: 99%

Influence of the fiber diameter and surface roughness of electrospun vascular grafts on blood activation

et al. 2012

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Section: Discussionmentioning

confidence: 99%

Influence of the fiber diameter and surface roughness of electrospun vascular grafts on blood activation

et al. 2012

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“…26 The inter fiber pore size and the distribution of the pores can be easily achieved by sacrificial components or adjusting the electrospinning conditions. 27 The HFDF cells seeded on the PPCL mat also showed similar infiltration into the inter fiber space with even distribution of cells.…”

Section: Morphological Evaluation Of Coculture On Ppclmentioning

confidence: 81%

Self Organized Skin Equivalent by Epithelial and Fibroblast Cells on Polycaprolactone Electrospun Mat with Porous Fibers

Kumar¹

2017

ATROA

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Tissue engineered skin substitutes are used in chronic, partial or full thickness wounds, and serve to augment the growth and repair of the skin at the wound site, by acting as a temporary or permanent skin replacement. In this study Polycaprolactone a biodegradable polymer was electrospun in a binary solvent system to form nonwoven mats with porous fibers (PPCL). These mats were hydrolysed with alkali to make them more hydrophilic and promote cell adhesion. The electrospun mat served as a scaffold for culture of human keratinocyte (HaCaT) and rabbit hair follicle dermal fibroblast cells (HFDF) cells. F-actin staining and SEM analysis showed that the cells exhibited their characteristic morphology, with good adhesion and spreading. Live dead staining confirmed the viability of the cells on the PPCL mat. A bioengineered construct of HaCaT and HFDF cells cultured on either side of the PPCL mat was generated in vitro. Light microscopy of Toluidine blue stained cryo sections of the construct reveal that the HaCaT cells formed intact multilayers, while the fibroblasts showed a more distributed growth pattern with migration into the mat, hence emulating the epidermis and dermis of the skin. The results were substantiated in the SEM micrographs and fluorescent images of DiI and DiO labeled cocultures. The PPCL mat cocultured with HaCaT and HFDF cells thus finds potential as a self organized tissue engineered skin substitute..

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“…This resulted in a higher density of fibers which are aligned on the wire than randomly oriented, scattered fibers at the gaps between the wires. Where the distance between the wires get larger, the difference in the density of fibers on the wires and the space between the wires increases 13 . Electrospinning jet using polycaprolactone solution was able to discern conductive lines with widths of 350 µm and spacing of 1.7 mm 14 .…”

Section: Introductionmentioning

confidence: 99%

Templated Roughness on the Surface of Polyamide Nanofibrous Mat by Mesh Electrospinning

Saadatmand¹,

Yazdanshenas²,

Khajavi³

et al. 2017

Orient. J. Chem

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In this study, it was aimed to present a nanofibrous scaffold with intentional roughness through a new electrospinning method called "mesh electrospinning method". Firstly, polyamide nylon 6 granules were dissolved in formic acid in different concentration (10-35 %w/v). A mesh template was placed between tip and collector in different distance (4 positions) in a conventional electrospinning line. The number of mesh layers and effects of processing parameters also considered. As it was expected, the results show that, increasing the polymer solution lead to increase fiber diameter, from 84 nm for 20% to 265 nm for 30%. The results showed that the layer just formed when the mesh was placed just on the collector. On the other hand, with increasing the number of mesh layers (1&3), roughness decreased (55-60%). It was also observed that the produced roughness is governed by the geometry of the mesh. To consider the ability for controlling the roughness, the produced roughness can be applied for drug delivery purposes transdermal drug delivery.

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Increasing electrospun scaffold pore size with tailored collectors for improved cell penetration

Cited by 222 publications

References 33 publications

Influence of the fiber diameter and surface roughness of electrospun vascular grafts on blood activation

Influence of the fiber diameter and surface roughness of electrospun vascular grafts on blood activation

Self Organized Skin Equivalent by Epithelial and Fibroblast Cells on Polycaprolactone Electrospun Mat with Porous Fibers

Templated Roughness on the Surface of Polyamide Nanofibrous Mat by Mesh Electrospinning

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