Increases in miR-124-3p in Microglial Exosomes Confer Neuroprotective Effects by Targeting FIP200-Mediated Neuronal Autophagy Following Traumatic Brain Injury

Li, Dai; Huang, Shan; Yin, Zhenyu; Zhu, Jia‐Lin; Ge, Xintong; Han, Zhaoli; Tan, Jin; Zhang, Shishuang; Zhao, Jing; Chen, Fanglian; Wang, Haichen; Lei, Ping

doi:10.1007/s11064-019-02825-1

Cited by 89 publications

(48 citation statements)

References 77 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In support of these findings, Yang et al (182) showed that EV miR-124 treatment enhanced hippocampal neurogenesis and functional recovery by promoting the M2 polarization of microglia, the effect of which was produced through inhibition of the Tolllike receptor 4 (TLR4 pathway). In a subsequent study, Li et al (181) showed that increased miR-124-3p in MDE promoted neurite outgrowth via miR-124-3p transfer into neurons, thereby inhibiting neuronal autophagy and protecting again against nerve injury.…”

Section: Microglial Extracellular Vesiclesmentioning

confidence: 98%

Extracellular Vesicle Proteins and MicroRNAs as Biomarkers for Traumatic Brain Injury

et al. 2020

View full text Add to dashboard Cite

Traumatic brain injury (TBI) is a heterogeneous condition, associated with diverse etiologies, clinical presentations and degrees of severity, and may result in chronic neurobehavioral sequelae. The field of TBI biomarkers is rapidly evolving to address the many facets of TBI pathology and improve its clinical management. Recent years have witnessed a marked increase in the number of publications and interest in the role of extracellular vesicles (EVs), which include exosomes, cell signaling, immune responses, and as biomarkers in a number of pathologies. Exosomes have a well-defined lipid bilayer with surface markers that reflect the cell of origin and an aqueous core that contains a variety of biological material including proteins (e.g., cytokines and growth factors) and nucleic acids (e.g., microRNAs). The presence of proteins associated with neurodegenerative changes such as amyloid-β, α-synuclein and phosphorylated tau in exosomes suggests a role in the initiation and propagation of neurological diseases. However, mechanisms of cell communication involving exosomes in the brain and their role in TBI pathology are poorly understood. Exosomes are promising TBI biomarkers as they can cross the blood-brain barrier and can be isolated from peripheral fluids, including serum, saliva, sweat, and urine. Exosomal content is protected from enzymatic degradation by exosome membranes and reflects the internal environment of their cell of origin, offering insights into tissue-specific pathological processes. Challenges in the clinical use of exosomal cargo as biomarkers include difficulty in isolating pure exosomes, variable yields of the isolation processes, quantification of vesicles, and lack of specificity of exosomal markers. Moreover, there is no consensus regarding nomenclature and characteristics of EV subtypes. In this review, we discuss current technical limitations and challenges of using exosomes and other EVs as blood-based biomarkers, highlighting their potential as diagnostic and prognostic tools in TBI.

show abstract

Section: Microglial Extracellular Vesiclesmentioning

confidence: 98%

Extracellular Vesicle Proteins and MicroRNAs as Biomarkers for Traumatic Brain Injury

et al. 2020

View full text Add to dashboard Cite

show abstract

“…However, brain extract from a mouse with Traumatic Brain Injury (TBI) can be used to influence microglial cells to secrete miR-124-3p enriched exosomes. These miR-124-3p enriched exosomes can treat TBI by targeting focal adhesion kinase family-interacting protein (FIP200) [ 47 ].…”

Section: Disease Treatment Using Mirna-enriched Evsmentioning

confidence: 99%

Therapeutic miRNA-Enriched Extracellular Vesicles: Current Approaches and Future Prospects

Munir

Yoon

Ryu

2020

Cells

View full text Add to dashboard Cite

Extracellular vesicles (EVs) are 50–300 nm vesicles secreted by eukaryotic cells. They can carry cargo (including miRNA) from the donor cell to the recipient cell. miRNAs in EVs can change the translational profile of the recipient cell and modulate cellular morphology. This endogenous mechanism has attracted the attention of the drug-delivery community in the last few years. EVs can be enriched with exogenous therapeutic miRNAs and used for treatment of diseases by targeting pathological recipient cells. However, there are some obstacles that need to be addressed before introducing therapeutic miRNA-enriched EVs in clinics. Here, we focused on the progress in the field of therapeutic miRNA enriched EVs, highlighted important areas where research is needed, and discussed the potential to use them as therapeutic miRNA carriers in the future.

show abstract

“…MiR-21-5p was able to directly affect the Rab11a 3′UTR region to reduce its translation and further suppress neuronal autophagy mediated by Rab11a [ 169 ]. Another study by Li D et al provided evidence that these effects were achieved mainly via upregulation of exosomal miR-124-3p, and that focal adhesion kinase family interacting protein of 200 kDa (FIP200), which plays a key role in trauma-induced autophagy [ 170 ].…”

Section: Extracellular Vesicles As Therapy In Tbimentioning

confidence: 99%

Extracellular Vesicles miRNA Cargo for Microglia Polarization in Traumatic Brain Injury

2020

View full text Add to dashboard Cite

Traumatic brain injury (TBI) is one of the major causes of death and disability worldwide, and despite its high dissemination, effective pharmacotherapies are lacking. TBI can be divided into two phases: the instantaneous primary mechanical injury, which occurs at the moment of insult, and the delayed secondary injury, which involves a cascade of biological processes that lead to neuroinflammation. Neuroinflammation is a hallmark of both acute and chronic TBI, and it is considered to be one of the major determinants of the outcome and progression of disease. In TBI one of the emerging mechanisms for cell–cell communication involved in the immune response regulation is represented by Extracellular Vesicles (EVs). These latter are produced by all cell types and are considered a fingerprint of their generating cells. Exosomes are the most studied nanosized vesicles and can carry a variety of molecular constituents of their cell of origin, including microRNAs (miRNAs). Several miRNAs have been shown to target key neuropathophysiological pathways involved in TBI. The focus of this review is to analyze exosomes and their miRNA cargo to modulate TBI neuroinflammation providing new strategies for prevent long-term progression of disease.

show abstract

Increases in miR-124-3p in Microglial Exosomes Confer Neuroprotective Effects by Targeting FIP200-Mediated Neuronal Autophagy Following Traumatic Brain Injury

Cited by 89 publications

References 77 publications

Extracellular Vesicle Proteins and MicroRNAs as Biomarkers for Traumatic Brain Injury

Extracellular Vesicle Proteins and MicroRNAs as Biomarkers for Traumatic Brain Injury

Therapeutic miRNA-Enriched Extracellular Vesicles: Current Approaches and Future Prospects

Extracellular Vesicles miRNA Cargo for Microglia Polarization in Traumatic Brain Injury

Contact Info

Product

Resources

About