Increases in concentrations of potassium and bicarbonate ions promote acquisition of motility in vitro by Japanese eel spermatozoa

Ohta, Hiromi; Ikeda, Kazuo; Izawa, Takeshi

doi:10.1002/(sici)1097-010x(19970201)277:2<171::aid-jez9>3.0.co;2-m

Cited by 52 publications

(39 citation statements)

References 18 publications

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“…Media used for the spermatozoa activation of the Japanese eel spermatozoa have a pH of 8.2 (Miura et al 1995;Ohta et al 1997a). This value is near to the 8.1-8.2 measured in the sperm (Ohta et al , 2001 and similar to the 8.1-8.2 used for sperm diluting media Izawa 1995, 1996;Ohta et al 1997bOhta et al , c, 2001.…”

Section: As Wellmentioning

confidence: 88%

Physio-Chemical Characteristics of Seminal Plasma and Development of Media and Methods for the Cryopreservation of European eel Sperm

Asturiano

Pérez

Garzón

et al. 2004

Fish Physiol Biochem

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The high sperm density, together with the short spermatozoa swimming time, makes European eel sperm manipulation and assessment for quality difficult. Two diluting media (K15 and K30) previously designed for Japanese eel sperm were tested. After 24 h, European eel sperm showed significant reduction in the percentage of motile spermatozoa after activation and different motility parameters (VAP, angular velocity; VCL, curvilinear velocity; VSL, straight line velocity; BCF, beating cross frequency), concluding that these media are not suitable to preserve the sperm of this species. After a hormonal treatment to induce spermiation, sperm volume, density and motility were recorded at weekly samplings. The variation of the osmolality (325-330 mOsm kg )1 ), pH (8.4-8.6) and the ionic composition (concentration of Na + , K + , Mg 2+ and Ca 2+ ) of the seminal plasma were registered. Physio-chemical results were related with sperm quality throughout the treatment, to determine which must be the suitable characteristics of one extender for the sperm of this species, and to find the best conditions to obtain suitable cryopreservation media for European eel sperm. K + concentration increased, while Ca 2+ and Mg 2+ concentrations showed a progressive reduction in correlation with the sperm quality improvement. Na + showed a decreasing, but not significant tendency. P1 and P2 freezing media were designed considering the physio-chemical parameters as well as the ionic composition shown by the best quality sperm samples, and then compared with the previously described solutions, TNK and K30. Sperm quality was determined, checking the percentage of motile spermatozoa and motility parameters using computer-assisted sperm analysis (CASA) software. Samples were frozen after dilution (1:5, 1:20, 1:100) in different freezing media supplemented with 10% dimethyl sulfoxide (DMSO). After thawing, samples frozen with low dilution ratio (1:5) in TNK and P1 media showed higher, although not significant, spermatozoa survival (35.5±14.5 and 36.6±6.7%). The addition of L-a-phosphatidylcholine to the media seems to have a positive effect, as reported in the Japanese eel.

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Section: As Wellmentioning

confidence: 88%

Physio-Chemical Characteristics of Seminal Plasma and Development of Media and Methods for the Cryopreservation of European eel Sperm

Asturiano

Pérez

Garzón

et al. 2004

Fish Physiol Biochem

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“…1). Osmolarity of cryoprotective solution was higher than SSW (Table 1); such factor would have been influencing motility and time of activity of A. purpuratus sperm like it was observed in other species (Morisawa & Suzuki, 1980;Chambeyron & Zohar, 1990;Ohta et al, 1997). Minimal or no toxicity of the cryoprotective solution on A. purpuratus sperm could have been favored by inclusion of cryoaditives (sucrose and egg hen yolk), since spermatic motility in tests performed with only ME 2 SO, was always less than fresh sperm (Dupré et al, 1999).…”

Section: Discussionmentioning

confidence: 99%

Morphological alterations in cryopreserved spermatozoa of scallop Argopecten purpuratus

Espinoza

Valdivia

Dupré

2010

lajar

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ABSTRACT. The present work identifies and quantifies the morphological alterations of scallop Argopecten purpuratus spermatozoa caused by long-term cryopreservation. Percentages of motility, fertilization and injured spermatozoa were quantified by optic microscopy and scanned electron microscopy. These parameters were evaluated in sperm without treatment (CTR), spermatozoa incubated in cryoprotective solution but not freezed (ICS) and freezed-thawed spermatozoa (FTS). Spermatozoa of ICS treatment remained motile longer than those of CTR, whereas those of FTS treatment were lowest. Morphology of the spermatozoa was affected in several ways by the freeze-thawing treatment; some had their head deformed or swollen, others had their cell membrane folded or broken; acrosome reaction; anomalous positions or absence of mitochondria as well as broken, stiff or loss of lineal structure of tail. CTR and ICS treatments had higher percentages of undamaged sperm (87.7% and 79.0% respectively), while FTS samples had 14.2% of undamaged sperm. The tail was the spermatic structure most commonly injured in FTS (77.0%), the percentage of sperm with head injury was 55.1% and with acrosome reaction was 28.7%, whereas middle piece was affected in 23.9% of sperm. Percentages of fertilization were 68.3%, 67.9% and 58.2% for CTR, ICS and FTS respectively, which were not significantly different. There was a higher correlation between injuries and motility than between injuries and fertilization success. Correlation between motility and fertilization was low (0.605 and 0.668 with motility at 5 and 30 min, respectively). Keywords: cryopreservation, Argopecten purpuratus, scallop, sperm, Chile. Alteraciones morfológicas en espermatozoides criopreservados de concha de abanicoArgopecten purpuratus RESUMEN. El presente trabajo identifica y cuantifica las alteraciones morfológicas en espermatozoides de concha de abanico A. purpuratus causadas por la criopreservación en nitrógeno líquido. Porcentajes de motilidad, fecundación de ovocitos frescos y espermatozoides lesionados (en cabeza, acrosoma, pieza media y flagelo) fueron determinados bajo microscopía óptica y electrónica de barrido. Estos parámetros fueron evaluados en un control sin tratamiento (CTR), espermatozoides incubados en solución crioprotectora pero sin congelamiento (ICS) y espermatozoides congelados-descongelados (FTS). Los espermatozoides del tratamiento ICS mostraron mayor motilidad que de CTR, mientras que la motilidad de los espermatozoides del tratamiento FTS fue la más baja. La morfología externa de los espermatozoides fue afectada de varias formas por el congelamiento-descongelamiento; cabeza deformada o hinchada, membrana celular plegada o rota, reacción acrosómica, anormal posición o ausencia de las mitocondrias, ruptura, rigidez o pérdida de la estructura lineal del flagelo. El CTR e ICS presentó los mayores porcentajes de espermatozoides ilesos (87,7% y 79,0% respectivamente), mientras que las muestras de FTS tuvieron 14,2% de espermatozoides ilesos. El flagelo fue la e...

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“…An understanding of the physical nature and chemical constituents of SVP is fundamental to know its functional role in sperm storage and motility, and fertilization. Several studies (Table 1) have described the physical and chemical composition of testicular fluid or seminal plasma (Kruger et al 1984;Lahnsteiner et al , 1993aLahnsteiner et al , 1994Lahnsteiner et al , 1995Lahnsteiner et al , 1996Kara et al 1996;Ohta et al 1997;Tan-Fermin et al 1999), but only a few are available on seminal vesicle fluid (SVF) (Steyn and van Vuren 1987;Lahnsteiner et al 1992a;Chowdhury and Joy 2001a;Mansour et al 2004). However, a comprehensive analysis of SV secretion is not yet available.…”

Section: Secretionsmentioning

confidence: 99%

“…Various lines of evidence from light and electron microscopic, histochemical/histoenzymological, and physiological studies strongly indicate that the efferent duct system is not a mere passive exit duct, but participates in several functions related to the secretion and ionic regulation of seminal fluid, steroid production, the storage of spermatozoa, the secretion of nutrients and pheromones, and the synthesis of proteins and enzymes (Kruger et al 1984;Lahnsteiner et al , 1992aLahnsteiner et al , 1993aLahnsteiner et al , 1994Lahnsteiner et al , 1995Lahnsteiner et al , 1996Linhart et al 1991;Billard et al 1995;Kara et al 1996;Ohta et al 1997;Lahnsteiner 2003).…”

Section: Introductionmentioning

confidence: 99%

“…Such modifications have been reported in blennies, gobies, charids, pimelodids, and catfishes and are described variously as testicular glands, testicular blind pouches, and seminal vesicles or sperm duct glands (Colombo and Burighel 1974;Patzner and Seiwald 1987a, b;de Jonge et al 1989;Patzner 1987, 1989;Lahnsteiner et al , 1992aFishelson et al 1994;Joy and Singh 1998;Singh and Joy 1998a;Meisner et al 2000;Richtarski and Patzner 2000;Hamlett et al 2002;Reardon et al 2002;Cruz and Santos 2004). Various functions are attributed to these, such as differentiation of spermatids, storage and nutrition of spermatozoa, secretion of sialomucins, proteins and enzymes, storage of lipids and phospholipids, production of steroids and steroid conjugates, phagocytosis of residual germ cells, pheromonal function, and the enhancement of sperm motility and fertilization efficiency (Sundararaj and Nayyar 1969a;Lahnsteiner et al , 1992avan den Hurk and Resink 1992;Ohta et al 1997;Singh and Joy 1999;Chowdhury and Joy 2001a;Mansour et al 2004) (Fig. 1).…”

Section: Introductionmentioning

confidence: 99%

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Seminal vesicle and its role in the reproduction of teleosts

Chowdhury

Joy

2007

Fish Physiol Biochem

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One mouse click on fish seminal vesicle (SV) in Pub-Med reveals a limited number of articles that give various pieces of information on its restricted distribution, origin, structure, relation with testis, and physiology. Although in the last decade significant progress has been made with respect to the nature and multiple functions of SV secretions, and new aspects are steadily being uncovered, much still remains to be known before we can realize its potential application in fisheries. This review is an attempt to provide an update on recent research on various aspects of the SV and its secretory products in teleosts. The available data suggest a significant role of SV and its products in the maturation and nutrition of sperm cells, in the maintenance of their integrity and viability, and the enhancement of spawning performance and fertilization.

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Increases in concentrations of potassium and bicarbonate ions promote acquisition of motility in vitro by Japanese eel spermatozoa

Cited by 52 publications

References 18 publications

Physio-Chemical Characteristics of Seminal Plasma and Development of Media and Methods for the Cryopreservation of European eel Sperm

Physio-Chemical Characteristics of Seminal Plasma and Development of Media and Methods for the Cryopreservation of European eel Sperm

Morphological alterations in cryopreserved spermatozoa of scallop Argopecten purpuratus

Seminal vesicle and its role in the reproduction of teleosts

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