Increased Walleye Egg Hatch and Larval Survival after Protease Treatment of Eggs

Krise, William F.; Bulkowski-Cummings, Loretta; Shellman, A. David; Kraus, Keith A.; Gould, Rowan W.

doi:10.1577/1548-8640(1986)48<95:iwehal>2.0.co;2

Cited by 20 publications

(27 citation statements)

References 9 publications

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“…Krise et al (1986) pointed out that the adhesive layer surrounding the chorion is particularly susceptible to viral, fungal, and bacterial pathogens and that removal of this can significantly reduce the development of such pathogens. Furthermore, routine disinfection and removal of dead eggs during egg incubation are important for improved biosecurity, both of which can be made easier and more effective by incubating eggs in suspension.…”

Section: Discussionmentioning

confidence: 99%

Removal of the adhesive gum layer surrounding naturally fertilised ballan wrasse (Labrus bergylta) eggs

et al. 2016

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Commercial production of ballan wrasse (Labrus bergylta) as a cleaner fish for the removal of sea lice (Lepeophtheirus salmonis) from farmed salmonids (Salmo salar) has increased due to its proven efficiency. One bottleneck in commercial hatchery production is working with the benthic adhesive eggs, which makes disinfection and incubation of eggs challenging; therefore, this study aimed to find a chemical or enzymatic treatment and process to remove the adhesive gum layer. Naturally spawned eggs were collected from artificial spawning substrates up to 24 hours post spawning from wild caught broodstock kept in captivity at the Marine Harvest, Machrihanish facility. Four treatments were tested: tannic acid (0.2, 0.1, and 0.05 %), sodium sulfite (2, 1, and 0.5 %), L-cysteine (2, and 1 %), and enzyme alcalase® (4.0, 3.0, 2.0, 1.0, and 0.5 %) in vitro. Eggs were exposed for 25 minutes while being continually agitated, and the proportion of "degummed" eggs was counted at the end of each time period. Enzyme alcalase® was the only treatment that proved successful in degumming eggs, with the time to complete degumming (≥ 96 %) inversely related to enzyme concentration. Complete degumming occurred between 15 and 30 minutes for all enzyme alcalase® dose rates. Mean hatch rates for eggs treated with enzyme alcalase ® were not compromised by the treatment and in the highest dose tested were actually found to be higher in treated eggs (78.9 ± 2.4 %) than controls (71.3 ± 3.3 %).The use of enzyme alcalase® has proven effective in degumming ballan wrasse eggs without affecting hatch rates. However, translation of this method to in situ degumming and thus removal of eggs from spawning substrate on farm remains to be standardised.

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Section: Discussionmentioning

confidence: 99%

Removal of the adhesive gum layer surrounding naturally fertilised ballan wrasse (Labrus bergylta) eggs

et al. 2016

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“…It has been used as antifungal treatment in egg incubation of different freshwater fish species such as: Clarias garepinus, Cyprinus carpio, Oncorhynchus mykiss and Ictalurus punctatus (Marking et al, 1994;Froelich and Engelhardt, 1996;Schreier et al, 1996;Weirich and Tiersch, 1997;Khodabandeh and Abtahi, 2006;Rasowo et al, 2007). Increased salinity was also applied in intensive culture for increased survival and osmoregulatory of percid larvae (Krise et al, 1986;Guo et al, 1993;Bein and Ribi, 1994) and for control of fish diseases in intensive freshwater finfish culture (Mifsud and Rowland, 2008). Weirich and Tiersch (1997) found positive effect of increased salinity (application of 1 g NaCl·L −1 ) in hatchery water on the hatching rate in I. punctatus.…”

Section: Discussionmentioning

confidence: 99%

Sodium chloride as effective antifungal treatment for artificial egg incubation inAustropotamobius pallipes

Policar

Smyth²,

Flanigan³

et al. 2011

Knowl. Managt. Aquatic Ecosyst.

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Key-words:formaldehyde, fungicide treatment, hatching, sodium chloride, white-clawed crayfishIn this study, sodium chloride at three different concentrations, 30 000 ppm (S30), 60 000 ppm (S60) and 90 000 ppm (S90), and formaldehyde at one concentration, 3000 ppm (F), were tested as antifungal chemicals during artificial incubation (AI) of Austropotamobius pallipes eggs. Two treatments were tested without chemicals as control groups with (R) and without (C) the removal of dead eggs. After AI, formaldehyde treatment ensured high survival of stage 1 (89.7 ± 2.3%) and stage 2 (85.5 ± 2.4%) of juveniles. However, comparable survival rate to stage 1 and stage 2 (85.5 ± 5.5% and 80.6 ± 3.2%) were also found in the treatment with the highest sodium chloride concentration (S90). Significantly lower survival rate of juveniles (stage 1: 60.6−70.3% and stage 2: 56.1−67.3%) were evident in groups S60, S30 and R. However, group R demanded high labor and related costs. The lowest juvenile survival levels to stage 1 (46.4 ± 8.2%) and stage 2 (45.2 ± 6.8%) were observed in treatments without fungicide chemicals and removal of dead eggs (C). RÉSUMÉLe chlorure de sodium comme traitement antifongique efficace dans l'incubation artificielle des oeufs d'Austropotamobius pallipes Mots-clés :formaldéhyde, traitement antifongique, éclosion, chlorure de sodium, écrevisse à pieds blancs Dans cette étude, le chlorure de sodium à trois différentes concentrations, 30 000 ppm (S30), 60 000 ppm (S60) et 90 000 ppm (S90), et le formol à une concentration de 3000 ppm (F) ont été testés comme produits antifongiques pendant l'incubation artificielle (AI) d'oeufs d'Austropotamobius pallipes. Deux traitements ont été testés sans produits antifongiques comme témoins avec (R) et sans (C) retrait des oeufs morts. Après incubation artificielle, le traitement au formol assure une survie élevée au stade 1 (89,7 ± 2,3 %) et au stade 2 (85,5 ± 2,4 %) des juvéniles. Toutefois, un taux de survie comparable au stade 1 et 2 (85,5 ± 5,5 % et 80,6 ± 3,2 %) a été trouvé avec le traitement à la plus forte concentration en chlorure de sodium (S90). Un taux de survie significativement plus faible des juvéniles (stade 1 : 60,6−70,3 % et stade 2 : 56,1− 67,3 %) est observé pour les groupes S60, S30 et R. Mais le groupe R demande un gros travail coûteux. Les survies les plus faibles des juvéniles au stade 1 (46,4 ± 8,2 %) et au stade 2 (45,2 ± 6,8 %) sont observées en l'absence de traitement chimique et de retrait des oeufs (C).

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“…In the Gulf Acipenser eggs, the use of fullers and clay decreased the duration of egg de-adhesion procedure to half (Elizabeth, 2001). In white sturgeon Acipenser transmontanus, a combination of urea/salt or sodium sulfite accompanying tannic acid decreased considerably the duration of egg deadhesion procedure (Krise et al, 1986). Demska-Zake et al (2005) observed a significant decrease in the duration of egg de-adhesion of walleye, pike perch (Sander lucioperca) when he used tannic acid.…”

Section: Discussionmentioning

confidence: 99%

EFFECT OF TWO EGG DE-ADHESION METHODS ON REPRODUCTIVE SUCCESSES OF BELUGA Huso huso

Hosseini¹,

Khara²

2015

CJF

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Increased Walleye Egg Hatch and Larval Survival after Protease Treatment of Eggs

Cited by 20 publications

References 9 publications

Removal of the adhesive gum layer surrounding naturally fertilised ballan wrasse (Labrus bergylta) eggs

Removal of the adhesive gum layer surrounding naturally fertilised ballan wrasse (Labrus bergylta) eggs

Sodium chloride as effective antifungal treatment for artificial egg incubation inAustropotamobius pallipes

EFFECT OF TWO EGG DE-ADHESION METHODS ON REPRODUCTIVE SUCCESSES OF BELUGA Huso huso

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