Increased tubulin messenger RNA in the frog retina after optic nerve transection

Mizobuchi, Tokihide; Yagi, Yasuyuki; Mizuno, A; Matsuzaki, Hiroshi; Matsuda, Makoto

doi:10.1016/0304-3940(88)90561-7

Cited by 5 publications

(6 citation statements)

References 6 publications

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“…However, changes in the mRNA levels of both tubulin and actin as a function of time in the bullfrog retina during the regenerative process have not been observed so far. We reported previously that a rapid and transient increase of a-tubulin mRNA was observed in the bullfrog retina about 1-2 h after optic nerve transection, but no specific change in actin mRNA was seen (Mizobuchi et al, 1988). In this study, we confirmed the regeneration of retinotectal fibers following optic nerve transection in bullfrog and investigated further the long-term effect of optic nerve transection by Northern and dot blot hybridization.…”

supporting

confidence: 76%

“…There was a dramatic and transient increase in the a-tubulin mRNA level during the few hours immediately following optic nerve transection (Mizobuchi et al, 1988) and another slow steady increase in the mRNA level over the 28-day experimental period following the treatment (Fig. 3).…”

Section: Discussionmentioning

confidence: 94%

“…On the other hand, the changes in actin mRNA levels were fairly different from those in tubulin mRNA levels. Though the significant changes in actin mRNA levels were not detected within 4 h of optic nerve transection (Mizobuchi et al, 1988), the mRNA increased significantly at about 7 days after optic nerve transection (Fig. 4A, lanes 5 and 6).…”

Section: Changes In Tubulin and Actin Mrna Levels After Optic Nerve T...mentioning

confidence: 91%

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Changes in α‐Tubulin and Actin Gene Expression During Optic Nerve Regeneration in Frog Retina

Mizobuchi

Yagi

Mizuno

1990

Journal of Neurochemistry

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The optic nerve of the bullfrog was transected and the regeneration process was investigated. We previously reported that alpha-tubulin mRNA in the retina increased to a maximum 1-2 h after optic nerve transection with no specific change in actin mRNA. In the present investigation, we examined the long-term effect of optic nerve transection. Northern blot analysis revealed that alpha-tubulin mRNA increased again gradually after the rapid and transient increase and actin mRNA increased to a maximum at 7 days (more than twofold compared to the control retinas). The period during which actin mRNA reaches a maximal increase almost corresponds to the time lag between the axotomy and the initiation of axonal outgrowth. The main cytoskeletons of neuronal growth cones have been shown to consist of actin-containing microfilaments. Therefore, the transient increase of actin mRNA may have a relationship to the initial outgrowth of axons. On the other hand, the rapid and transient increase of alpha-tubulin mRNA observed in our previous studies is probably one of the initial responses of retinal ganglion cells to the axotomy, and the gradual increase in alpha-tubulin mRNA observed in this study can probably be interpreted as provision of the structural materials necessary for axonal elongation.

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supporting

confidence: 76%

Section: Discussionmentioning

confidence: 94%

Section: Changes In Tubulin and Actin Mrna Levels After Optic Nerve T...mentioning

confidence: 91%

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Changes in α‐Tubulin and Actin Gene Expression During Optic Nerve Regeneration in Frog Retina

Mizobuchi

Yagi

Mizuno

1990

Journal of Neurochemistry

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“…Their roles in the ocular system have been proposed in previous studies. During the optic nerve regeneration process in bullfrog, there was a rapid and transient increase in the messenger RNA of α-tubulin gene . Also, using Northern blot analysis and in situ hybridization, it was found that the rat homologue of α-tubulin was highly expressed during the extension of neuronal processes .…”

Section: Resultsmentioning

confidence: 99%

“…regeneration process in bullfrog, there was a rapid and transient increase in the messenger RNA of R-tubulin gene. 54 Also, using Northern blot analysis and in situ hybridization, it was found that the rat homologue of R-tubulin was highly expressed during the extension of neuronal processes. 55 In addition, -tubulin was also found to express in the differentiating retinal neuroblasts, and it may be important in the retinal ganglion cell differentiation.…”

Section: Decyder Analysis After the Second Phase Electrophoresismentioning

confidence: 99%

Application of Fluorescence Difference Gel Electrophoresis Technology in Searching for Protein Biomarkers in Chick Myopia

Lam

et al. 2007

J. Proteome Res.

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The lens-induced myopia (LIM) in response to concave lens (negative lens) is a well established animal model for studying myopia development. However, the exact visual and neurochemical signaling mechanisms involving myopic eye growth are yet to be elucidated. The feasibility of applying a novel two-dimensional fluorescence difference gel electrophoresis technique for global protein profilings and a search for differential protein expressions in LIM were explored in the present study. Two-dimensional polyacrylamide gel electrophoresis was performed employing a "minimal Lysine labeling" approach and a reverse CyeDye experimental protocol using retinal tissue from chicks. The retinal protein profiles between myopic and control eyes were found to be very similar. More than a thousand protein spots could be detected on a 2D gel. Sixteen and ten protein spots were found to be up-regulated and down-regulated respectively in the myopic eyes according to our preset criteria with the inclusion of an internal pool standard. About 65% of those filtered spots could be successfully identified by peptide mass fingerprinting by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry . Most of the differentially expressed proteins were found to be related to cytoskeletal or oxidative functions. According to the prediction of subcellular locations, most of them (about 84%) were classified as cytoplasmic proteins. The cellular functions for those differentially expressed proteins were reported and their possible involvements in the compensated eye growth were discussed. We have optimized a workable protocol for the study of the differential retinal protein expressions in the LIM using 2D-DIGE approach which was shown to have a number of advantages over the traditional 2D electrophoresis technique.

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Molecular biology of cell activation

Kaczmarek

Kamińska

1989

Experimental Cell Research

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Increased tubulin messenger RNA in the frog retina after optic nerve transection

Cited by 5 publications

References 6 publications

Changes in α‐Tubulin and Actin Gene Expression During Optic Nerve Regeneration in Frog Retina

Changes in α‐Tubulin and Actin Gene Expression During Optic Nerve Regeneration in Frog Retina

Application of Fluorescence Difference Gel Electrophoresis Technology in Searching for Protein Biomarkers in Chick Myopia

Molecular biology of cell activation

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