Molecular biology of cell activation

Kaczmarek, Leszek; Kamińska, Bożena

doi:10.1016/0014-4827(89)90415-1

Cited by 54 publications

(18 citation statements)

References 83 publications

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“…In conclusion, we would like to say that polyamines' influence on the structure ofcytoskeleton and, in consequence, on cell proliferation may be, at least in part, mediated through effects of polyamines on the expression of genes encoding major cytoskeletal proteins. The importance of this finding is further emphasized by the fact that it has been reported that several peaks of polyamine synthesis occur in the cell cycle; the first one after a few hours after mitogenic stimulation, and others preceding DNA synthesis and cell division [4,10]. Therefore, one may expect that depletion ofpolyamines ma~, impair several different components of the cell cycle, namely growth in size, DNA replication and cell division, as all these are dependent on the integrity of the ¢ytoskeletai structure which in turn depends on polyamines.…”

Section: The Concentrations Of Inhibitors Have Been Selected In Ourmentioning

confidence: 92%

“…The polyamines, spermidine and spermine, and their precursor putrescine, are ubiquitous components of mammalian cells [1]. Although the physiological functions of these compounds are still not well understood, several studies have shown that normal cellular growth and division require polyamines [2][3][4][5]. Direct support for this notion has been provided by experiments in which polyamine synthesis was prevented by the application of inhibitors [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

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Inhibitors of polyamine biosynthesis affect the expression of genes encoding cytoskeletal proteins

Kamińska¹,

Kaczmarek²,

Grzelakowska-Sztabert³

1992

FEBS Letters

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The pclyamines are ubiquitous components of mammalian cells. Those compounds have been postulated to play an important role in different cellular functions including the reorganization ofcytoskeleton associated with the cell cycle. In the studies reported here, it was found that inhibitors of polyamine biosynthesis, methylglyoxal.bis[quanylhydrazone] (MGBG) and difluoromethylornithine (DFMO), prevent mitogen.indueed accumulation of mRNAs encoding major cytoskeletal eomponems, fl-actin and a-tubulin, in mouse splenocytes. These findings suggest mechanisms through which polyamines may exert their effects on the cytoskeleton integrity.

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Section: The Concentrations Of Inhibitors Have Been Selected In Ourmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Inhibitors of polyamine biosynthesis affect the expression of genes encoding cytoskeletal proteins

Kamińska¹,

Kaczmarek²,

Grzelakowska-Sztabert³

1992

FEBS Letters

Self Cite

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“…The cell transition from the quiescent to the proliferative state (GO->GI) requires an increase in the rate of RNA and protein synthesis (GI phase) necessary for cells to replicate their DNA (S phase) (64)(65)(66)(67)(68)(69)(70). We analyzed the effect of bFGF (1 and 25 ng/ml) on RNA, protein and DNA synthesis by using synchronized HUVE cells prior to or after exposure of the cells to TCM (Fig.…”

Section: Methodsmentioning

confidence: 99%

Cytokines from activated T cells induce normal endothelial cells to acquire the phenotypic and functional features of AIDS-Kaposi's sarcoma spindle cells.

Fiorelli¹,

Gendelman²,

Samaniego³

et al. 1995

J. Clin. Invest.

148

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Kaposi's sarcoma (KS) is a proliferative disease of vascular origin particularly frequent in HIV-1-infected homosexual men (AIDS-KS) and characterized by proliferating spindleshaped cells, angiogenesis, and inflammatory cell infiltration. Previous work has suggested that KS spindle cells are of endothelial cell origin and that chronic immune activation via the release of inflammatory cytokines may cooperate with basic fibroblast growth factor (bFGF) and the HIV-1 Tat protein in the induction and progression of AIDS-KS. Invest. 1995Invest. .95:1723Invest. -1734

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“…Ineffective behavior during the first trials of learning might lead to neuronal changes, e.g. changes in "neuronal phenotype" [49] and create "the potential" for formation of the following memory [50]. Neuronal gene expression changes during the first stage of learning might only prime the subsequent long-term changes and form a background for the following selection of specific neuronal groups as proposed by the selection theories of learning [4] [51].…”

Section: Discussionmentioning

confidence: 99%

Clustered c-Fos Activation in Rat Hippocampus at the Acquisition Stage of Appetitive Instrumental Learning

Svarnik¹,

Alexandrov²,

Anokhin³

2015

JBBS

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To address the issue of how hippocampal neurons are involved into learning progress, we studied c-Fos expression in rat hippocampal subfields at different stages of appetitive instrumental learning. To model the first stage of learning, we pre-trained animals to approach the lever to obtain the food, and then made this behavior ineffective by not reinforcing it during the last session ("mismatch" group). Another group just acquired lever-pressing behavior at that day ("acquisition" group). Animals of the third group performed this well-trained behavior ("performance" group). The number of Fos-positive neurons in all hippocampal regions of the "mismatch" group animals was higher than in the ones of the home cage control group animals. The number of Fos-positive neurons was increased in CA1 and CA3 areas, but not in the dentate gyrus of both the "acquisition" and "performance" group animals as compared with the control group. We also found segmented c-Fos expression, which was more evident in "acquisition" group animals. Thus, our results suggest that during the first (mismatch) stage of learning hippocampal neurons are activated in an equally distributed manner. The following clustered pattern of activated CA1 neurons during the acquisition stage may reflect specialization of these neurons in respect to the specific lever-pressing behavior.

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Molecular biology of cell activation

Cited by 54 publications

References 83 publications

Inhibitors of polyamine biosynthesis affect the expression of genes encoding cytoskeletal proteins

Inhibitors of polyamine biosynthesis affect the expression of genes encoding cytoskeletal proteins

Cytokines from activated T cells induce normal endothelial cells to acquire the phenotypic and functional features of AIDS-Kaposi's sarcoma spindle cells.

Clustered c-Fos Activation in Rat Hippocampus at the Acquisition Stage of Appetitive Instrumental Learning

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