Increased transcription of a major stress gene in spontaneously hypertensive mice.

Hamet, Pavel; Malo, Danielle; Tremblay, Johanne

doi:10.1161/01.hyp.15.6.904

Cited by 42 publications

(18 citation statements)

References 31 publications

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“…It is important, however, to keep in mind that our data did not conclude that there is no relationship between hypertension per se and regulation of HSP70. Hamet et al 19 have reported heightened expression of renal HSP70 in the genetic hypertensive animals only after heat exposure of the whole body. Thus, it is possible that although NE treatment did not increase renal HSP70 expression, it may modulate HSP70 expression in response to some physiological stimuli such as heat shock.…”

Section: Discussionmentioning

confidence: 99%

Regulation and Localization of HSP70 and HSP25 in the Kidney of Rats Undergoing Long-Term Administration of Angiotensin II

et al. 2002

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Abstract-Various renal insults result in induction of heat shock protein (HSP) expression within the kidney. Some of the HSPs induced in that manner are postulated to have renoprotective effects via either chaperoning actions or antioxidative properties. We have previously reported that long-term angiotensin (Ang) II administration induces the expression of renal HSP32, also known as heme oxygenase-1 (HO-1). Here, we investigated the regulation of expression and localization of other HSPs, including HSP70, HSP25, and ␣B-crystallin, in the kidney of rats undergoing long-term administration of Ang II (0.7 mg · kg. Immunoblot analysis demonstrated that Ang II increased renal expression of HSP70 and HSP25, as well as HO-1, but that expression of ␣B-crystallin was unaffected by this treatment. The Ang II-induced increase in renal HSP70 and HSP25 was dependent on the angiotensin type 1 receptor activation but not on hypertension per se. Immunohistochemistry revealed that HSP70 and HSP25 were expressed in the medullar regions and in the renal arterial wall in the kidney of control rats. After Ang II infusion, signals for HSP70, HSP25, and HO-1 proteins increased in intensity in the endothelium and medial smooth muscle of the renal artery. In addition, all of these HSPs were induced in proximal renal tubular epithelial cells from the same segments, suggesting that similar mechanisms are responsible for upregulating these HSPs. Our data show that Ang II infusion induces renal HSP70 and HSP25, as well as HO-1, and that Ang II can induce expression of these HSPs in renal cells in a pressor-independent manner. 4 Previous studies have demonstrated that several HSPs, including HSP32 (also known as heme oxygenase-1, HO-1), 5 HSP25, HSP60, HSP70/HSP72, HSP73, 6 and ␣B-crystallin, 7 are present in the kidney.HO-1/HSP32, an inducible form of HO, catalyzes the rate-limiting step in the degradation of heme. Induction of renal HO-1 has been shown to ameliorate renal injury induced by rhabdomyolysis. 8 Gene ablation of HO-1 exacerbated cisplatin-induced renal tubular injury. 9 Thus, the intrarenal HO system is thought to play a role in protecting renal function against various renal insults. The inducible form of HSP70 is also shown to be regulated in the kidney in response to stimuli such as ischemia 7 and hyperthermia. 10 Overexpression of transfected HSP70 in the renal cells was found to have a protective action against cisplatin toxicity, oxidative injury, 11 and hyperthermia. 12 HSP25 and ␣B-crystallin are both members of the low-molecular-weight HSP family. They can be phosphorylatable at various amino acid positions, although their phosphorylation may not be essential for their chaperoning properties. 13 Renal expression of HSP25 and ␣B-crystallin is known to be regulated, 7,14 but their precise biological role in the kidney remains unclear.We have recently shown that HO-1 is induced in tubular epithelial cells in response to administration of angiotensin (Ang) II and that the increased levels of HO-1 may play a role in ameli...

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Section: Discussionmentioning

confidence: 99%

Regulation and Localization of HSP70 and HSP25 in the Kidney of Rats Undergoing Long-Term Administration of Angiotensin II

et al. 2002

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“…Enhanced expression of Hsp70 has been detected in hypertensive experimental animals (31,32 ). Pockley et al (15 ) reported that Hsp65 and Hsp70 antibody titers were increased in subjects with hypertension, whereas Hsp60 antibody titers were similar to those in (11 ).…”

Section: Discussionmentioning

confidence: 99%

Joint Effects of Antibody to Heat Shock Protein 60, Hypertension, and Diabetes on Risk of Coronary Heart Disease in Chinese

Zhang

Cheng

et al. 2008

Clinical Chemistry

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“…HSP27 may elicit phosphorylation related to the signalling function when normal growing cells are exposed to various environmental stimuli [46][47][48][49][50][51]. We have reported earlier that SHR VSMC in culture display a shorter transition from G1 to S [15]. The relationship between the abnormal growth of hypertensive VSMC and the abnormal expression of HSP27 gene may be implicated in a common step in the process of signal transduction.…”

Section: Discussionmentioning

confidence: 99%

“…In the past few years, we have focused on the general stress response of hypertensive patients, animals and cell lines [14][15][16][17][18]. We believe that the abnormal response to various environmental stresses in hypertension is genetically-determined and that genes induced by heat or other stressors, modify the development of hypertension.…”

Section: Introductionmentioning

confidence: 99%

“…HSP27, HSPT0 and HSP104 have been found to be involved in the regulation of thermotolerance [25][26][27][28]. We have reported previously that abnormal HSP70 gene expression is evident in hypertensive subjects and animals after exposure to heat and other stressful stimuli [15,16,29] and the restriction fragment length polymorphism (RFLP) of the HSP70 locus segregates with an increment of blood pressure in 30 recombinant inbred strains (RIS) of rats [18].…”

Section: Introductionmentioning

confidence: 99%

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Candidate genes of hypertension with defective environmental expression

et al. 1995

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Previous studies in our laboratory have demonstrated that the thermosensitivity locus cosegregates with blood pressure and that the elevated expression and restriction fragment length polymorphism of HSP70 gene are associated with hypertension. Cell protection against environmental stressors such as heat and chemicals is often accompanied by up-regulated expression of a wide spectrum of heat shock genes(HSP). To further investigate the interrelation between HSP expression and blood pressure regulation, we employed an effective method of cloning 2 potential hypertension-related HSPs. Synthetic oligonucleotides corresponding either to a highly-conserved region of the known HSP family or a repetitive sequence in the proteinencoding gene were used as target primers for polymerase chain reaction (PCR). cDNA prepared from heat-stressed and non-stressed vascular smooth muscle cells (VSMC) of Brown Norway rats (BN.lx) and spontaneously hypertensive rats (SHRp) respectively served as template in the reaction. The PCR products were subsequently analyzed in a single-stranded conformational polymorphism (SSCP) electrophoresing system. Differential gene expression in BN. lx and SHRp was seen on autoradiographs of SSCP gel by comparing the migration patterns of PCR-amplified

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Increased transcription of a major stress gene in spontaneously hypertensive mice.

Cited by 42 publications

References 31 publications

Regulation and Localization of HSP70 and HSP25 in the Kidney of Rats Undergoing Long-Term Administration of Angiotensin II

Regulation and Localization of HSP70 and HSP25 in the Kidney of Rats Undergoing Long-Term Administration of Angiotensin II

Joint Effects of Antibody to Heat Shock Protein 60, Hypertension, and Diabetes on Risk of Coronary Heart Disease in Chinese

Candidate genes of hypertension with defective environmental expression

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