Increased Smooth-Surface Caries Incidence in Gnotobiotic Rats Immunized with Actinomyces viscosus

Burckhardt, Johann Jakob; Guggenheim, B

doi:10.1159/000260435

Cited by 8 publications

(4 citation statements)

References 3 publications

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“…1, 2, 4, and 5). No dis- (Table 1) matched the weights of rats in the isolators at a similar age (7). The rate of bone loss in first and second molars decreased after 72 days of age, particularly in immunized and infected rats (Fig.…”

Section: Discussionmentioning

confidence: 90%

Alveolar bone loss in rats after immunization with Actinomyces viscosus

Burckhardt¹,

Gaegauf-Zollinger²,

Schmid³

et al. 1981

Infect Immun

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We investigated a possible cause-and-effect relationship between sensitization against Actinomyces viscosus Nyl and destructive periodontal disease in RIC-Sprague-Dawley rats. Germfree rats (66) were either immunized with A. viscosus Nyl (day 20) or orally infected with A. viscosus Nyl (days 38 and 39) or both. We measured alveolar bone loss in maxillary and mandibular molars, in vitro Tlymphocyte responsiveness, and serum antibody titers. In immunized and monoassociated rats bone loss in both jaws progressed rapidly between days 37 and 72, whereas the rate of further resorption decreased until day 100. In monoassociated rats, development of bone loss was much slower, and the maxiimum resorption measured was, at best, half of the bone loss compared with the former group. However, no amplification of bone loss by immunization was observed in a second experiment using 63 conventional rats kept in relative gnotobiosis. Antibody titers to A. viscosus Nyl in gnotobiotic monoassociated rats were higher in immunized animals, whereas no difference was found in the respective groups of the relative gnotobiotic experiment. The fact that immunization more than doubled alveolar bone loss in gnotobiotic monoassociated rats confirms the allergic nature of the disease. The lack of such an effect under conventional conditions points to suppressor mechanisms whose decrease might convert stable periodontal lesions into progressive ones.

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Section: Discussionmentioning

confidence: 90%

Alveolar bone loss in rats after immunization with Actinomyces viscosus

Burckhardt¹,

Gaegauf-Zollinger²,

Schmid³

et al. 1981

Infect Immun

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show abstract

“…Salivary antibody was restricted to the IgA isotype. Similarly, Burckhardt and Guggenheim (9) reported that germfree RIC-Sprague-Dawley rats immunized with lyophilized A. viscosus NY-1 and monoassociated with the same bacterium accumulated as much plaque as sham-immunized controls. Again, 60 days after infection they found no differences in the serum antibody titers of the immunized and sham-immunized groups.…”

mentioning

confidence: 86%

Effect of local and parenteral immunization on implantation of Actinomyces viscosus T6 in rats

Olsson¹,

Hsu²,

Kingman³

et al. 1985

Infect Immun

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Groups of rats immunized in the vicinity of the major salivary glands or immunized intraperitoneally with Acdinomyces viscosus T6 and their sham-immunized controls were infected with the homologous bacterium. Significantly higher levels of salivary and serum antibody were induced by intraperitoneal than by salivary gland immunization. There were also significant inverse correlations between the levels of salivary and serum antibody and the levels of implantation of A. viscosus T6. The level of implantation of A. viscosus T6 was significantly lower in the immunized animals than in the controls. However, antibody had limited capacity to inhibit the establishment of this bacterium.

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“…(Fine, et al, 2001) (Schreiner, et al, 2003) Microbial attachment factors essential for colonization of animal tooth and tissue Can be analyzed (Schreiner, et al, 2003) (Sharma, et al, 2001) Microbial attachment and colonization can be related to antibody response and bone loss. (Burckhardt & Guggenheim, 1980(Schreiner, et al, 2003Sharma, et al, 2001) Analysis of effect of antagonistic or symbiotic organisms on growth and survival of test microbe (Hillman, et al, 2000) …”

Section: Bacterial Virulence Factorsmentioning

confidence: 99%

The use of rodent models to investigate host–bacteria interactions related to periodontal diseases

Graves

Fine

Teng

et al. 2008

J Clinic Periodontology

335

370

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Even though animal models have limitations they are often superior to in vitro or clinical studies in addressing mechanistic questions and serve as an essential link between hypotheses and human patients. Periodontal disease can be viewed as a process that involves four major stages: bacterial colonization, invasion, induction of a destructive host response in connective tissue and a repair process that reduces the extent of tissue breakdown. Animal studies should be evaluated in terms of their capacity to test specific hypotheses rather than their fidelity to all aspects of periodontal disease initiation and progression. Thus, each of the models described below can be adapted to test discrete components of these four major steps, but not all of them. This review describes five different animal models that are appropriate for examining components of host-bacteria interactions that can lead to breakdown of hard and soft connective tissue or conditions that limit its repair as follows: the mouse calvarial model, murine oral gavage models with or without adoptive transfer of human lymphocytes, rat ligature model and rat Aggregatibacter actinomycetemcomitans feeding model.

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Increased Smooth-Surface Caries Incidence in Gnotobiotic Rats Immunized with Actinomyces viscosus

Cited by 8 publications

References 3 publications

Alveolar bone loss in rats after immunization with Actinomyces viscosus

Alveolar bone loss in rats after immunization with Actinomyces viscosus

Effect of local and parenteral immunization on implantation of Actinomyces viscosus T6 in rats

The use of rodent models to investigate host–bacteria interactions related to periodontal diseases

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