Increased serum levels of solublel-selectin (CD62L) in patients with active systemic lupus erythematosus (SLE)

Abstract

SUMMARYThe adhesion molecule L-selectin (CD62L) mediates lymphocyte recirculation and leucocyte rolling on vascular endothelium at sites of inflammation. Serum levels of soluble L-selectin (sL-selectin) were measured in patients with SLE in order to relate these levels to clinical activity and immunological parameters. An ELISA was used to detect the soluble form of human L-selectin (CD62L) in 42 patients with SLE and in 33 healthy individuals. The mean Ϯ s.e.m. values of sL-selectin were 1285 Ϯ 121 ng/ml for patients with SLE and 986 Ϯ 180 ng/ml for healthy blood donors, but there was no significant difference. When patients with active SLE were analysed, higher levels of circulating sL-selectin were found when compared with patients without activity (1497 Ϯ 167 ng/ml versus 941 Ϯ 150 ng/ml; P ¼ 0·028). We found a significant correlation between the levels of sL-selectin and of dsDNA antibodies (r ¼ 0·36, P ¼ 0·044) and between levels of sL-selectin and SLE Disease Activity Index (SLEDAI) score (r ¼ 0·42, P ¼ 0·003). Patients with active SLE studied cross-sectionally showed significant elevations of sL-selectin (CD62L) compared with controls. Thus, the levels of this soluble adhesion molecule correlated with active disease and levels of anti-dsDNA antibodies.

A Functional Role for Circulating Mouse L-Selectin in Regulating Leukocyte/Endothelial Cell Interactions In Vivo

“…The LAM1-102 mAb identifies an epitope within the epidermal growth factor/short consensus repeat regions of L-selectin, whereas the LAM1-116 mAb binds an epitope located within the lectin domain (5). Comparable results were obtained when the LAM1-101 mAb replaced the LAM1-102 mAb, which binds to a similar region of L-selectin (data not shown) (45). The presence of sL-selectin was readily detected in serum from normal adult B6 mice, with a mean concentration of 1.7 Ϯ 0.1 g/ml (Fig.…”

“…In the current study, mouse serum sL-selectin levels were quantified using a previously described human serum standard that contained a known concentration of sL-selectin (26,28). Others have used recombinant human L-selectin as a standard for measuring human sLselectin levels (45). Therefore, it is likely that humans and mice have similar, high levels of serum sL-selectin that are likely to influence leukocyte migration.…”

“…Significantly increased levels of sL-selectin are found in the serum of patients suffering from chronic myeloid and lymphocytic leukemia (94 -96), sepsis (28,47), HIV infection (28), atopic dermatitis (50), severe psoriasis (97), and active systemic lupus erythematosus (45). High sL-selectin levels in cases of hematological malignancies or inflammatory disorders may explain why leukocytes in these patients circulate for longer periods of time as compared with normal individuals and are delayed in their entry into tissues.…”

The pathophysiology of photosensitivity in lupus erythematosus

“…These cells interact via cutaneous lymphocyte associated antigen (CLA) on their surface with E-selectin molecules on dermal microvascular endothelial cells. Levels of soluble E-selectin correlate with active disease and levels of dsDNA antibodies in patients with SLE (248). E-selectin can be upregulated by UVB (249) and its expression is increased in CCLE and SLE photoinduced lesions (250).…”

No association between E- and L-selectin genes and SLE: soluble L-selectin levels do correlate with genotype and a subset in SLE

“…Small-scale studies in lupus patients indicate that levels of soluble and membrane-bound E-selectin 9,10 and soluble L-selectin (sL-selectin) may be associated with SLE and disease activity. 11,12 The sL-selectin is derived from calcium-dependent endoproteolytic cleaving of membrane-bound protein from activated lymphocytes and from rolling neutrophils. 13,14 sL-selectin is present at high concentrations in the serum of healthy humans (B1.0 mg/ml) and retains functional activity perhaps inhibiting interactions involving its cell surface counterpart.…”