Increased serum immunoglobulin E concentrations in venereal diseases.

Green, R L; Scales, R W; Kraus, Stephen J.

doi:10.1136/sti.52.4.257

Cited by 12 publications

(10 citation statements)

References 16 publications

(10 reference statements)

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“…The results of determinations of IgE concentrations support the findings of Green et al,7 although in our study IgE concentrations in patients with primary and secondary syphilis were evaluated as a group without further differentiation of the stage of disease. Taken together, these studies indicate an IgE response in Tpallidum infection.…”

Section: Discussionsupporting

confidence: 91%

Antitreponemal IgE in early syphilis.

Bos¹,

Hamerlinck²,

Cormane³

1980

Sexually Transmitted Infections

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Marked slides (see below) containing eight circles of 0 6 cm diameter were stored in 96Vo alcohol. Fresh Treponema pallidum suspension (see Materials) was thoroughly mixed and centrifuged at 200 x g for 10 minutes to free the suspension of cellular (rabbit) debris. The suspension was then diluted with distilled water until 30-40 micro-organisms per field were visible at x 400 magnification by darkground microscopy and allowed to mix for 10 minutes. Before use, these slides were wiped with absorbent paper. On each circle, 0-01 ml of the diluted antigen suspension was applied and allowed to dry at 37°C for 60-90 minutes. After drying the preparations were fixed in acetone for 10 minutes and stored in polyethylene sealed phials at -70°C until required.TEST SERA Ten millilitres of peripheral blood was collected aseptically and allowed to clot for one hour at room temperature; it was then centrifuged for 10 minutes at 400 x g. The serum was pipetted off and divided into 0 2-ml aliquots. The sera were stored at -70°C until required. No inactivation was carried out. Before use the test sera were diluted in sorbent (see Materials) and incubated for 30 minutes at room temperature. INCUBATION OF TEST SAMPLESFrom each test sample (patient serum diluted 1/5 in sorbent, control serum diluted 1/5 in sorbent, patient serum diluted in phosphate-buffered saline (PBS) for estimation of titre), 0-02 ml was applied to the antigen circle and incubated in a humid atmosphere at 37°C for 30 minutes. The slides were then gently

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Section: Discussionsupporting

confidence: 91%

Antitreponemal IgE in early syphilis.

Bos¹,

Hamerlinck²,

Cormane³

1980

Sexually Transmitted Infections

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“…Since the discovery of human IgE by Ishizaka et al (1966) and delineation of its biologic properties ), many investigators have sought to uncover a useful role for antibodies in this immunoglobulin class by measuring its concentration in serum and other body fluids in states of health and disease. Augmented IgE biosynthesis has now been associated with the atopic diseases (Berg & Johansson 1969); certain bacterial (Green et al 1976), fungal (Jones et al 1974), and parasitic ) diseases; and a number of the primary immunodeficiency diseases (Buckley et al 1972, Waldmann et al 1972, Buckley & Fiscus 1975. Although all of these represent conditions of ill health, it does not seem likely from a teleological standpoint that IgE would bave survived in evolution on the basis of its harmful properties.…”

Section: Introductionmentioning

confidence: 99%

Abnormalities in the Regulation of Human IgE Synthesis

Buckley

Becker

1978

Immunological Reviews

217

109

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“…Of particular interest is a report that the mean serum IgE level was higher in a group of patients with gonorrhoea compared with a group of normal individuals (Green et al, 1976 response to infections in rats by parasites (Perrudet-Badoux et al, 1976). Also, IgE antibodies are formed in mice immunised with water soluble antigens isolated from certain Gram-negative bacteria (Danneman and Michael, 1976).…”

Section: Introductionmentioning

confidence: 99%