Antitreponemal IgE in early syphilis.

Abstract

Marked slides (see below) containing eight circles of 0 6 cm diameter were stored in 96Vo alcohol. Fresh Treponema pallidum suspension (see Materials) was thoroughly mixed and centrifuged at 200 x g for 10 minutes to free the suspension of cellular (rabbit) debris. The suspension was then diluted with distilled water until 30-40 micro-organisms per field were visible at x 400 magnification by darkground microscopy and allowed to mix for 10 minutes. Before use, these slides were wiped with absorbent paper. On each circle, 0-01 ml of the diluted antigen suspension was applied and allowed to dry at 37°C for 60-90 minutes. After drying the preparations were fixed in acetone for 10 minutes and stored in polyethylene sealed phials at -70°C until required.TEST SERA Ten millilitres of peripheral blood was collected aseptically and allowed to clot for one hour at room temperature; it was then centrifuged for 10 minutes at 400 x g. The serum was pipetted off and divided into 0 2-ml aliquots. The sera were stored at -70°C until required. No inactivation was carried out. Before use the test sera were diluted in sorbent (see Materials) and incubated for 30 minutes at room temperature. INCUBATION OF TEST SAMPLESFrom each test sample (patient serum diluted 1/5 in sorbent, control serum diluted 1/5 in sorbent, patient serum diluted in phosphate-buffered saline (PBS) for estimation of titre), 0-02 ml was applied to the antigen circle and incubated in a humid atmosphere at 37°C for 30 minutes. The slides were then gently

Cytokine gene expression in skin of susceptible guinea‐pig infected with Treponema pallidum

“…22 in the USA reported high levels of IgE concentration in serum samples of 50 patients with primary and secondary syphilis, whereas Bos et al . 23 in the Netherlands demonstrated significantly higher ( P <0·05) levels of IgE antitreponemal antibodies in patients with primary and secondary syphilis when compared to healthy controls.…”

IgE Anti‐Borrelia burgdorferi Components (p18, p31, p34, p41, p45, p60) and Increased Blood CD8+CD60+ T Cells in Children with Lyme Disease

“…The clinical implications of these IgE antiviral Abs are, as of yet, unknown. It has been shown that syphilis patients produce IgE Abs to T. pallidum [9, 10]; detection of anti‐ Chlamydia pneumoniae IgE has been reported in children with reactive airway disease [29]. IgE Abs to bacterial infections, such as staphylococcal disease have been reported in patients with allergies [30], and atopic dermatitis [31].…”

“…Although earlier studies of Benach et al [9] have shown the presence of specific IgE Abs to B. burgdorferi in patients with Lyme disease [9], neither epitopic specificity nor the long‐term presence of these Abs, were reported. In addition, IgE anti‐spirochete Abs ( Treponema pallidum ) and spirochete tick vectors ( Ixodes ricinus and Argus reflexus ) have also been reported [10–12]. However, the role of IgE anti‐spirochete Abs and the persistence of IgE anti‐spirochete Abs remain unclear.…”

Immunoglobulin-bearing lymphoid cells in primary syphilis. Quantitative and elution studies.

“…Studies of the humoral response have shown that after infection immunoglobulins of different classes (IgG, IgA, IgM, and IgE) are extensively produced and their raised concentrations in serum and (at least partial) antitreponemal specificity have been demonstrated. [2][3][4][5][6] Although T pallidum is present in the body after inoculation, there is often a delay before specific immunoglobulins can be detected. A temporary delay or block in the differentiation of antigenstimulated B lymphoid cells into immunoglobulinsecreting plasma cells may be the origin of this delay.…”