Increased Reissner's fiber material in the subcommissural organ and ventricular area in bile duct ligated rats

The neuroactive neurosteroid Dehydroepiandrosterone Sulfate (DHEAS) modulates the serotonergic system within the dorsal Raphe nucleus and the cerebrospinal fluid release of Reissner’s fiber in rat

“…The specificity of the immunoreactive materials was tested following subjection of the slides to the same immunohistochemical procedure as described above and using the pre-immune serum or omitting of the primary antibodies. These tests showed that the primary antibodies used against 5-HT and RF display specific labelling as has been previously published [28,34].…”

“…The specificity of the immunoreactive materials was tested following the subjection of the slides to same immunohistochemical protocol described above by either using the preimmune serum or omitting of the primary antibodies. These tests showed that both primary antibodies used against RF and 5-HT display specific labelling [34]. Quantification of the immunoreactive materials was performed according to the protocol published by Vilaplana and Lavialle 1999 [43].…”

“…This brain gland releases glycoproteins of high molecular weight into the cerebrospinal fluid (CSF) where they aggregate to form Reissner's fiber (RF) [26]. Several roles have been proposed for this gland such as water homeostasis [27,28], neuronal survival [29], detoxification [30], monoamines clearance in the CSF [31], lordosis [32], in the pathophysiology of hydrocephalus [33] and possibly of hepatic encephalopathy [34]. Previous works have shown that the SCO receives several neuronal innervations of GABAergic [35,36], noradrenergic and dopaminergic [27] and serotonergic nature [28,34,37].…”

“…Several roles have been proposed for this gland such as water homeostasis [27,28], neuronal survival [29], detoxification [30], monoamines clearance in the CSF [31], lordosis [32], in the pathophysiology of hydrocephalus [33] and possibly of hepatic encephalopathy [34]. Previous works have shown that the SCO receives several neuronal innervations of GABAergic [35,36], noradrenergic and dopaminergic [27] and serotonergic nature [28,34,37]. The latter is the most influential system upon the secretory activity of the SCO in different mammalian species [34,[38][39][40][41].…”

“…Previous works have shown that the SCO receives several neuronal innervations of GABAergic [35,36], noradrenergic and dopaminergic [27] and serotonergic nature [28,34,37]. The latter is the most influential system upon the secretory activity of the SCO in different mammalian species [34,[38][39][40][41]. Thereby, we aimed, throughout the present investigation, to test the possible modulator effects of the neurosteroid DHEAS on the secretory activity of the SCO in the normal rat, together with the assessment of serotonin expression in the dorsal raphe nucleus and the innervating fibers reaching the SCO in the same experimental conditions, by means of an immunohistochemical approach.…”